Your search resulted in 325 documents. Displaying 25 entries per page.
Synthetic Oxalate/ß-glucan Fungal Extracellular Matrix Demonstrates Potential Inhibition of Extracellular Enzyme Diffusion into Wood Cell Walls, and Calls into Question the Role of Enzymes in Wood Decay
2025 - IRG/WP 25-11053
ß-glucan is the major component of the extracellular matrix (ECM) of many fungi, including wood degrading fungi. Many of these species also secrete oxalate into the ECM. Our research demonstrates that ß-glucan forms a novel, previously unreported, hydrogel at room temperature with oxalate. This…
B Goodell, G A Tompsett, G Perez-Gonzalez, K Mastalerz, M Timko
Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
…centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia placenta extracellular metabolites. This matrix was also visible in the secondary wall of degraded birch wood. Antisera…
Y S Kim, B Goodell, J Jellison
Effect of wood polymers degradation during heat treatment on extracellular enzymatic activities involved in beech degradation by Trametes versicolor
2008 - IRG/WP 08-40392
Effect of heat treatment on extracellular enzymes involved in wood degradation by Trametes versicolor was investigated. Heat-treated and untreated beech blocks were exposed to T. versicolor on malt agar medium and extracellular enzymatic activities investigated. A strong ABTS oxidizing…
S Lekounougou, G Nguila Inari, M Pétrissans, S Dumarçay, J P Jacquot, E Gelhaye, P Gérardin
Sequestration of copper ions by the extracellular mucilaginous material (ECMM) of two wood rotting basidiomycetes
2004 - IRG/WP 04-10533
The radial growth rate of colonies originating from either whole or ECMM-free inocula of Coriolus versicolor was investigated. The presence of ECMM allowed colonies to maintain higher growth rates than those form ECMM-free inocula up to 2 mM CuSO4 in the mediu…
D Vesentini, D J Dickinson, R J Murphy
Effects of biocides on the extracellular mucilaginous material (ECMM) produced by two wood rotting basidiomycetes
2003 - IRG/WP 03-10469
Growth and production of extracellular mucilaginous material (ECMM) by the brown-rot fungus Gloeophyllum trabeum (FPRL 108 N) and the white-rot fungus Coriolus versicolor (CTB 863 A) was assessed in liquid culture, supplemented with the biocides CuSO4 and cyproconazole.
The…
D Vesentini, D J Dickinson, R J Murphy
Extracellular mucilage (ECM) in wood decay basidiomycetes
2002 - IRG/WP 02-10439
The ability of wood decay basidiomycetes to produce extracellular mucilage (ECM) and its relationship with total biomass production is being investigated. Growth and ECM production by the brown-rot fungus Gloeophyllum trabeum (FPRL 108 N) and the white-rot fungus Coriolus versicolor (CTB 863 A) was assessed in…
D Vesentini, D J Dickinson, R J Murphy
Extracellular laccase production in brown-rot fungus Coniophora puteana
2001 - IRG/WP 01-10391
…brown-rot fungus, was investigated in terms of extracellular ligninolytic enzyme production. Three fungal strains of C. puteana were inoculated in liquid culture medium with sawdust of pine and oak wood. Extracellular laccase production was observed in some strains of C. puteana in the culture medium with…
Kwang-Ho Lee, Seung-Gon Wi, Yoon Soo Kim
SEM investigation of the production of extracellular mucilaginous material (ECM) by some wood inhabiting and wood decay fungi when grown in wood
1997 - IRG/WP 97-10193
Previous reports have illustrated the involvement of extracellular mucilage (ECM) in wood decay by Basidiomycetes. Its production is investigated in a range of stain, mould and soft rot fungi in Corsican pine, Scots pine and European beech. Fungi examined were: Chaetomium globosum, Ceratocystis coerulescens,…
A R Abu, D J Dickinson, R J Murphy
SEM investigation of the production of extracellular mucilaginous material (ECM) by some wood inhabiting and wood decay fungi when grown on inert surfaces
1997 - IRG/WP 97-10194
…wood decay fungi have been studied for their production of extracellular mucilage when grown on inert surfaces, for comparison with their growth on wood. The species tested were: Coniophora puteana, Coriolus versicolor, Chaetomium globosum, Trichoderma harzianum, Ceratocystis coerulescens, Ophiostoma piceae, Mucor…
A R Abu, R J Murphy, D J Dickinson
The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
…The generation of hydroxyl radical was mostly due to the extracellular low molecular weight substances that catalyzed redox reaction between electron donors and O2 to produce H2O2 via O2 and to reduce H2O2 to HO·.The substances reduced Fe(III) to Fe(II) and strongly absorbed Fe(II).
H Tanaka, S Itakura, A Enoki
Investigation of extracellular mucilaginous material in some wood decay fungi
1996 - IRG/WP 96-10188
The external morphology of the extracellular mucilagenous material (ECM) produced by Coriolus versicolor and Coniophora puteana during colonization of Scots pine and beech was studies using SEM. Specimens were examined in the frozen hydrated, freeze-dried and critical point dried state.…
A R Abu, D J Dickinson, R J Murphy
Extracellular substance from the white rot basidiomycete Irpex lacteus involved in wood degradation
1992 - IRG/WP 92-1571
…of the substance that produced and reduced H2O2 was found extracellularly in cultures containing wood than in cultures containing glucose. The extent of one-electron oxidation activity of the extracellular substance was correlated with that of the wood-degrading activity, but the extent of phenol oxidase activity…
H Tanaka, T Hirano, G Fuse, A Enoki
The influence of crystalline and amorphous cellulose on extracellular hydrogen peroxide production by brown-rot fungi
1991 - IRG/WP 1482
…of wood by wood-rotting fungi. The production of extracellular hydrogen peroxide was studied by a quantitative method which detects the oxidation of the 2,2-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) by H2O2 and horseradish peroxidase (HRP) in liquid culture medium. The carbon sources used were…
A-C Ritschkoff, L Viikari
Immunolocalization of extracellular metabolites from Tyromyces palustris
1991 - IRG/WP 1491
Polyclonal antisera produced to extracellular metabolites from the brown-rot fungus Tyromyces palustris was used in immunogold TEM studies. Gold labelling was detected in the fungal cell wall and extracellular slime layer but little or sporadic labelling was noted within the cytoplasm of…
Yoon Soo Kim
Extracellular hydrogen peroxide producing and hydrogen peroxide reducing compounds of wood decay fungi
1991 - IRG/WP 1516
Extracellular H2O2-producing and H2O2-reducing compounds were isolated from wood-containing cultures of all the white-rot and brown-rot fungi and Ascomycetes which well degraded wood, but were not detected in the culture of the fungi which degraded little…
A Enoki, G Fuse, H Tanaka
Control of sapwood-inhabiting fungi by fractionated extracellular metabolites from Coniophora puteana
1991 - IRG/WP 1494
The objective of this study was to test the fractionated metabolites released by Coniophora puteana for their antagonistic activity against the sapstain fungi Ceratocystis coerulescens and Aureobasidum pullulans, and the molds Asperigillus niger and Penicilliu…
S C Croan, T L Highley
Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips,…
F Green III, C A Clausen, M J Larsen, T L Highley
Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on S…
A Enoki, S Yoshioka, H Tanaka, G Fuse
The production of extracellular hydrogen peroxide by some brown-rot fungi
1990 - IRG/WP 1446
…of wood by wood-rotting fungi. The production of extracellular hydrogen peroxide was studied by detecting the oxidation of the chromogen 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) by H2O2 and horse radish peroxidase (HPR). ABTS and HPR were added to a solid wood based culture…
A-C Ritschkoff, L Paajanen, L Viikari
Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
…to lignin peroxidase in peripheral vesicular bodies. Extracellularly, and in solid wood samples manganese II peroxidase was found localized in all wood cell wall regions of either Betula verrucosa, Populus sp. or Fagus sylvatica decayed by either Phanerochaete chrysosporium or Lentinus edodes at both early and…
G F Daniel, B Pettersson, T Nilsson, J Volc
Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
…which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The…
J A Micales, A L Richter, T L Highley
The effect of tunicamycin on production and secretion of extracellular carbohydrate-degrading enzymes by Postia placenta
1988 - IRG/WP 1342
The extracellular carbohydrate-degrading enzymes of wood-decay fungi are usually heavily glycosylated and therefore stable under most denaturing conditions. It is unlikely that wood decay can be prevented by simply inactivating these enzymes. Tunicamycin, an…
J A Micales, T L Highley
Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to…
B Goodell, G F Daniel, J Jellison, T Nilsson
Extracellular layers of wood decay fungi and copper tolerance
1983 - IRG/WP 1180
Extracellular layers around the hyphae of brown, white and soft rot fungi have been examined using electron microscopy. These layers were isolated for identification. Particular interest was directed towards the extracellular layers of copper-tolerant soft…
D M Francis, L E Leightley
Extracellular osmiophilic particles in connection with brown rot and white rot
1982 - IRG/WP 1157
The way of attack on Pine woodblocks of two brown-rot fungi (Fomitopsis pinicola, Coniophora puteana) differing in their cellulase activities, and one white-rot fungus (Trametes hirsuta) has been investigated by transmission electron microscopy. Even at early…
K Messner, H Stachelberger