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A new concept of oxalic acid biosynthesis in physiology of copper-tolerant brown-rot fungi
2001 - IRG/WP 01-10394
Recently, a wide variety of roles of oxalic acid (oxalate) in wood decay systems have been receiving much attention. Copper tolerance of wood-rotting basidiomycetes has been believed to be due to the detoxification of copper wood preservatives by oxalate produced by these fungi. However, biochemical mechanism of oxalate biosynthesis in relation to physiology of wood-rotting fungi has not been eluc...
E Munir, T Hattori, M Shimada


Removal of heavy metals from treated wood using biological methods
2005 - IRG/WP 05-50226
Heavy metals were removed from wood treated with copper based preservatives using brown-rot fungus Fomitopsis palustris. The amount of effective elements removed by treatment methods was examined. The relationship between oxalic acid concentration and the amount of heavy metals removed from each treated wood was also investigated. The relationship between fungus weight and removal rate was also in...
Dong-won Son, Dong-heub Lee


Decay and termite durabilities of heat-treated wood
2004 - IRG/WP 04-40272
Decay and termite resistances of Plato-treated timbers were evaluated using a modified JIS decay test method, feeding test in a laboratory scale and a field exposure against Reticulitermes speratus (Kolbe) or Coptotermes formosanus Shiraki. The results showed that no significant decay durability of the treated timbers although mass loss from decay slightly decreased in case of treated timbers. ...
S Doi, K Hanataa, E Kamonji, Yuuji Miyazaki


Preservative ability of wood to be fixed hydroxyl apatite substituted for antimicrobial metals
2001 - IRG/WP 01-30272
We succeeded in forming hydroxy apatite (HAp) in wood. HAp is non-toxicity and safe. Preservative ability of the wood, which substituted one part of Ca of constituent element of this HA p for antibacterial metals was measured. When Ca was substituted for Ag or Zn, mass loss in decay by brown-rot fungus F. palustris was restrained in about 50% (Ag) ~30% (Zn) of value of control specimen. However, w...
Y Haruhiko, I Sumaru


Variation of natural durability of sugi (Cryptomeria japonica) wood in 15 clones examined by decay test (Preliminary report)
2004 - IRG/WP 04-10526
Natural durability of wood in 27 trees from 15 clones of sugi (Cryptomeria japonica)  was investigated by an accelerated decay test. Thirty years old trees were collected from a clonal trial in Ibaraki prefecture, Japan. Natural durability is usually evaluated in heartwood. Mass losses of heartwood caused by a brown rot decay fungus, Fomitopsis palustris and a white rot decay fungus, Py...
K Yamamoto, A Tamura, R Nakada


Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley


A possible role of unique TCA cycles in wood-rotting basidiomycetes
2003 - IRG/WP 03-10461
The copper tolerant brown-rot fungus, Fomitopsis palustris, acquires metabolic energy by use of the constitutively-occurring Kornberg’s glyoxylate cycle coordinating with oxalate biosynthesis and glucose oxidation (Erman Munir et al. Proc. Natl. Acad. Sci. USA, (2001) 98, 11126-11130). Furthermore, this fungus does not have the normal TCA cycle, lacking 2-oxoglutarate dehydrogenase which is a ke...
E Munir, T Hattori, M Shimada


Silicic acid-Boric acid complexes as wood preservatives
2001 - IRG/WP 01-30273
A silicic acid monomer aqueous solution (SAMS) or colloidal silicic acid solution (CSAS) was combined with various metal compounds or boric acid. Agents where SAMS or CSAS was combined with boric acid gave good protection against decay caused by the brown-rot fungus Fomitopsis palustris, the treated wood (Cryptomera japonica D. Don) specimens after the leaching test maintained a high resistance to...
H Yamaguchi


Chemically modified tannin and tannin-copper complexes as preservatives for wood
2001 - IRG/WP 01-30271
The efficacy of Mimosa tannin, chemically modified tannin, and tannin-copper complexes as wood preservatives was studied. When the tannin-ammonia-CuCl2 solutions were impregnated into wood specimens in a one-step procedure, a large quantity of the tannin-copper complex was fixed in the specimens. Little of the complex was leached from specimens by a weathering treatment, and these specimens showed...
H Yamaguchi


A physiological role of the glyoxylate and TCA cycles in fruitbody formation of the coppertolerant brown-rot fungus Fomitopsis palustris
2002 - IRG/WP 02-10430
Changes in activity of the representative enzymes involved in biosynthesis of oxalic acid (oxalate) and carbon metabolism of glucose were investigated in relation to the fruit body formation of the copper-tolerant brown-rot fungus Fomitopsis palustris. Changes in specific activities of the two glyoxylate (GLOX) cycle key enzymes (isocitrate lyase (ICL) and malate synthase (MS)), the two oxalate-fo...
Jeong-Jun Yoon, T Hattori, M Shimada


Sensitivity to Copper of Basidiospores from Copper Tolerant Fungi: Fomitopsis palustris and Oligoporus placentus
2010 - IRG/WP 10-10707
Copper continues to be an important fungicide in wood preservation. It is the primary component of the preservatives that have replaced chromated copper arsenate for treated wood in residential construction in North America. However, a co-biocide is normally needed to protect against copper tolerant organisms. Previous work has shown that the spores of at least one copper tolerant fungus, Oligopo...
C S Woo, P I Morris


Biological performance of wood- and bamboo-polypropylene composites: Effects of particle content, particle size and Zn borate
2012 - IRG/WP 12-40577
Particle content and size of wood material in wood plastic composites (WPCs) can affect efficacy of WPCs against fungi and termites. This study evaluated fungal and termite resistance of WPCs manufactured by using two different levels of particle content (50 and 70%) and three different particle sizes (30, 60, and 100 mesh). In fungal resistance tests, Tyromyces palustris, a standardized test fung...
S N Kartal, S Aysal, E Terzi, T Yoshimura, K Tsunoda


Characterization of Pectinases from Brown-rot Fungus Fomitopsis palustris
2019 - IRG/WP 19-10951
Brown-rot fungi occur on softwood used as building materials and cause destructive breakdown of wood structure. Therefore, a more accurate understanding is important from the perspective of wood protection. Previous studies have reported that hyphae of brown-rot fungi go through bordered pits on tracheids when the fungi grow into softwood [Francis W.M.R.Schwarze (2007)], and torus existing in the ...
Y Tanaka, N Konno, T Suzuki, N Habu


The evaluation of synergistic effects of chemicals on fungicidal efficacy in crossed-paper tests
1991 - IRG/WP 2383
The mixing effects of wood preservatives were evaluated using the crossed-paper technique. Two filter paper strips (0.7 x 8 cm²) were treated by soaking with different chemicals [fungicides, a termiticide (chlorpyrifos or phoxim), a surface-active agent, a synergistic agent, and a stabilizer], and placed at right angles to each other on a fully grown mycelial mat of a test fungus in a Petri dish....
Dong-heub Lee, K Tsunoda, M Takahashi


A laboratory method for assessing the effectiveness of fungicides in preventing the spread of decay fungi within packages of unseasoned lumber
1983 - IRG/WP 2202
To study the deterioration caused by decay fungi in the laboratory, a method for testing fungicides for their effectiveness in preventing spread of decay was devised. Some experiments using this method are reported here....
A J Cserjesi, E L Johnson, A Byrne


Fungicidal and termiticidal effectiveness of alkylammonium compounds
1983 - IRG/WP 3232
This paper is related to effectiveness of several AAC's against wood decay fungi and termites by Japanese standardized test methods....
K Tsunoda, K Nishimoto


First draft of a monographic card for Fomitopsis pinicola (Fr.) Karst
1980 - IRG/WP 196
P W Perrin


Cytochemical localization of hydrogen peroxide in brown rot fungus Tyromyces palustris by cerium chloride technique
1999 - IRG/WP 99-10299
Cerium chloride (CeCl3) was used to localize H2O2 cytochemically for studying relationship between ultrastructural and functional characteristics of cellulose degradation by brown rot fungi. This technique proved very useful in localizing discrete electron-densereactionproducts at high resolution with minimal nonspecific deposition. The cytochemical localization of extracellular H2O2 by CeCl3 usin...
Yoon Soo Kim, Seung-Gon Wi


Decay patterns observed in butylene oxide modified ponderosa pine attacked by Fomitopsis pinicola
1983 - IRG/WP 1183
Small blocks of ponderosa pine chemically modified by butylene oxide to three different weight percent gains (WPG) were decayed for 2 months with the brown rot fungus Fomitopsis pinicola. Wood substance loss and the type of decay pattern recognised were fairly similar both for control and blocks treated to 8 and 15 WPG. No difference in attack was observed between radial or tangential walls in lat...
T Nilsson, R M Rowell


Studies on the preservation of structrual plywood - Part 1: Decay resistance of structural plywood
1974 - IRG/WP 238
The weight loss and the decreases in the compression strength and in the modulus of elasticity were measured to determine the decay resistance of structural plywood (lauan). Test pieces (50x25xA mm³) were exposed to the wood destroying fungi (Coriolus versicolor and Coriolellus palustris) for 2-3 or 2-4 months. After exposure, the measurement of the compression strength was carried out on the pie...
K Minami, Y Kenjo, S Sugiyama


Lignin-copper, a new wood preservative without arsenic and chromium
1992 - IRG/WP 92-3702
A more environmentally sound treatment for wood with preservatives containing no arsenic or chromium, has been developed and studied on a laboratory scale. The method involves a first step impregnation with an aqueous solution containing modified, water-soluble kraft lignin followed by a second step involving impregnation with a copper salt solution to give fixation of the lignin into a water-inso...
B Ohlsson, R Simonson


Protection of Ochroma pyramidale from fungal decay with N,N-napthaloylhyroxylamine
1998 - IRG/WP 98-30182
Fungal decay of wood in service results in billions of dollars (U.S.) in losses annually. Recent environmental restrictions, both U.S. and international, are limiting and eliminating the use of broad-spectrum, heavy metal biocides for wood preservation. Restrictions result primarily from problems with disposal. New wood preservatives need to be developed and tested which specifically target key el...
F Green III, T L Highley


Immunogold labelling of size marker proteins in brown rot-degraded pine wood
1990 - IRG/WP 1428
Pine wood degraded by Fomitopsis pinicola was infiltrated with a mixture of ovalbumin (45 kDa) and myoglobin (16.7 kDa). After crosslinking of the proteins with glutaraldehyde and preparation for electron microscopy ultrathin sections were labeled with gold-conjugated antibodies. Simultaneous labeling of both proteins on the same section showed that at 50-70% weight loss ovalbumin did not penetrat...
E Srebotnik, K Messner


Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola
1988 - IRG/WP 1343
By applying immunoelectronmicroscopic methods, lignin peroxidase of the white rot fungus Phanerochaete chrysosporium has been localized in the cytoplasm of hyphae, close to the plasmalemma and on the plasmalemma. Infiltration of wood specimen with culture filtrates, concentrated 300-fold, gave clear information on the penetration of the enzyme into the wood cell wall. Penetration was restricted to...
E Srebotnik, K Messner


Fungal resistance of smoke-dried Cryptomeria japonica wood
1998 - IRG/WP 98-40118
Performance of smoke dried wood on fungal resistance was studied. The maximum temperature of the smoke seasoning was 80-90°C in the drying room and 70-80°C within the wood for 6 days during the treatment for 15 days. Decay resistance of smoke-dried Cryptomeria japonica wood was evaluated using a brown rot fungus, Tyromyces palustris. Weight losses of untreated wood, smoke-dried wood, and smoke-d...
K Yamamoto, I Momohara, T Nishimura


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