Your search resulted in 21 documents.
An anti-termite formulation for soil treatment with natural products and its efficacy against Coptotermes formosanus Shiraki
2003 - IRG/WP 03-30319
An anti-termite formulation of an environmental conservation type for soil treatment was developed. The formulation was composed of decanoic acid (n-capric acid, an fatty acid derived from coconut oil with ten carbons) as an active ingredient and other natural products. Experiments to examine the efficacy of the formulation against Coptotermes formosanus Shiraki were conducted at the termite field...
S Yoshida, T Nakagaki, A Igarashi, A Enoki
Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki
Difference of wood decay manner between brown-rot species
2019 - IRG/WP 19-10938
Wood blocks (Cryptomeria japonica) which were decayed by a Polyporales white-rot fungus (Trametes versicolor) and brown-rot fungi (Fomitopsis palustris, F. pinicola, and Wolfiporia cocos), respectively, were analyzed by X-ray diffraction and infrared spectroscopic methods followed by multivariate analysis. In the analyses, the differences in the cellulose crystallinities and infrared spectral patt...
R Kondo, Yo Horikawa, S Nakaba, K Ando, M Yoshida
FTIR analysis of wood blocks decayed by brown-rot fungi
2020 - IRG/WP 20-10959
Calibration curves of the relative lignin contents in Cryptomeria japonica decayed by brown-rot fungi were developed with Infrared Spectroscopy and Klason technique. First, wood decay test was conducted using brown-rot fungi (Fomitopsis palusris, F. pinicola, Wolfiporia cocos, Gloeophyllum trabeum, and Neolentinus suffrutescens) and white-rot fungus (Trametes versicolor) was used as a comparison. ...
R Kondo, Y Horikawa, R Kose, M Yoshida
Effect of MVOC exposure on mycelial growth of wood rotting fungi
2021 - IRG/WP 21-10977
It is well known that wood rotting fungi produce microbial volatile organic compounds (MVOCs) as metabolites. In our previous studies, we have found that some MVOCs produced by wood rotting fungi are common to fungal species tested in the studies, while others are specific to each species. Furthermore, it has been also shown that each wood rotting fungi do not always produce the same type of MVOCs...
S Horikawa, R Konuma, M Yoshida
Decomposition and metabolism of gaseous COS by wood rotting fungi
2021 - IRG/WP 21-10978
Previous studies on physiology of wood rotting fungi have mostly focused on the metabolisms of carbon and nitrogen sources. On the other hand, despite of the biological importance of minerals such as sulfur, our knowledge of their metabolic systems is limited. The sulfur source for wood-rotting fungi has been thought to be water-soluble sulfur compounds such as sulfate esters and cysteine, which a...
R Iizuka, O Iwao, Y Katayama, M Yoshida
The iron reduction by chemical components of wood blocks decayed by wood rotting fungi
2021 - IRG/WP 21-10979
Brown-rot fungi, a group of wood rotting fungi, is well known to be one of major microorganisms that cause the deterioration of wooden buildings in Japan and have been considered to use chelator-mediated Fenton (CMF) reaction in concert with hydrolytic and redox enzymes for degradation of wood cell wall. CMF can be described as a non-enzymatic degradation system that utilizes hydroxyl radicals pro...
R R Kondo, Y Horikawa, K Ando, B Goodell, M Yoshida
Metabolite analysis of fungal COS degradation and phylogenetic investigation of COSase genes
2023 - IRG/WP 23-11018
Fungi are the major species causing microbial deterioration that reduces the commercial value of wood, such as wood decay and surface contamination, so it is important to accumulate knowledge on the physiology of fungi in order to develop countermeasures against these problems. Most physiological studies of fungi that have been reported have concerned the metabolism of carbohydrates as a source of...
R Iizuka, S Hattori, I Ohtsu, D Hibbett, Y Katayama, M Yoshida
A novel cellulose-binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11019
Wood-rotting basidiomycetes are the major organisms decomposing wood in nature. They are classified into two groups based on their decay modes; white-rot fungi and brown-rot fungi. White-rot fungi secrete various cellulolytic enzymes during the wood degradation process. The enzymes are known to be often appended with a cellulose binding domain (CBD) which assists the activity of catalytic domain. ...
Y Kojima, N Sunagawa, M Aoki, M Wada, K Igarashi, M Yoshida
Morphological observation of wood at the early stages of decay in brown rot and white rot
2023 - IRG/WP 23-11020
Wood rotting fungi, the fungal species causing biodeterioration for wood building, are generally classified into white-rot, brown-rot and soft-rot fungi based on their decay modes. Since white-rot and brown-rot fungi are known to reduce wood strength significantly, it is important to clarify the mechanisms of their wood degradation. White-rot fungi reduce wood strength as the decay progress and de...
R Tsukida, T Hatano, Y Kojima, Y Horikawa, S Nakaba, R Funada, M Yoshida
The cellulose binding mechanism of a novel cellulose binding domain from the brown-rot fungus Gloeophyllum trabeum
2023 - IRG/WP 23-11021
In nature, wood decay is caused by various wood-rotting basidiomycetes. Wood-rotting basidiomycete are mainly divided into white-rot fungi and brown-rot fungi. Their main carbon source is cellulose of the wood cell wall during wood decay, and they produce a variety of enzymes to decompose cellulose. The cellulolytic enzymes often possess a cellulose binding domain (CBD) as an additional domain con...
M Aoki, Y Kojima, M Wada, M Yoshida
Effect of volatile organic compounds produced by wood rotting fungi on mycelial growth
2023 - IRG/WP 23-11023
Microorganisms such as fungi or bacteria produce volatile organic compounds (so-called MVOCs: Microbial Volatile Organic Compounds) as metabolites. Some MVOCs have been found to be biologically active, for example inhibition of spore germination, and when fungi of different species encounter each other, the MVOCs pattern change, suggesting that MVOCs play a role as a mediator for biological intera...
S Horikawa, R Konuma, M Yoshida
Research on gaseous COS degradation by brown-rot fungus Gloeophyllum trabeum
2024 - IRG/WP 24-11033
Physiological studies of wood rotting fungi have mostly focused on the metabolism of carbon and nitrogen sources, which constitute the dominants components of wood. On the other hand, despite the physiological importance of trace elements such as sulfur, studies on their acquisition sources and metabolic pathways are limited. Until now, wood rotting fungi have been thought to utilize slight amount...
R Iizuka, R Tsukida, Y Katayama, M Yoshida
A novel cellulose-binding domain from the brown-rot fungus that can be used to evaluate cellulose in wood
2024 - IRG/WP 24-11046
Wood-rotting basidiomycetes are the primary microorganisms that decay wood in nature. They are classified as white-rot fungi and brown-rot fungi by the difference in decaying types. White-rot fungi secrete a variety of cellulolytic enzymes during wood degradation. These enzymes often have an additional cellulose-binding domain (CBD) that adsorbs to the cellulose surface and localizes the catalytic...
Y Kojima, N Sunagawa, S Tagawa, T Hatano, S Nakaba, M Aoki, M Wada, K Igarashi, M Yoshida
Interaction of cellulolytic enzymes with fungal cell wall polysaccharides
2025 - IRG/WP 25-11071
Wood rotting fungi are the primary agents responsible for the decomposition of wood in natural environments. These fungi secrete a variety of enzymes to degrade the chemically and structurally resistant components of the wood cell wall. Some of these enzymes possess a carbohydrate-binding module (CBM), which enhances enzymatic efficiency by increasing their affinity to the substrate. Previously, w...
K Fukabori, N Hattori, Y Kojima, R Iizuka, M Yoshida
Physiological characterization of a key enzyme involved in gaseous COS assimilation in the filamentous fungus Trichoderma harzianum
2025 - IRG/WP 25-11072
Filamentous fungi are among the primary organisms responsible for the biological deterioration of wood, including wood decay and surface contamination. Therefore, understanding their physiological mechanisms is important for developing effective wood preservation strategies. Most physiological studies on filamentous fungi have focused on the metabolism of major elements, such as carbon in the form...
R Iizuka, A Ono, T Suzuki, Y Katayama, M Yoshida
Distribution of Crystalline Cellulose-Binding Domain CBM104 in Wood Rotting Fungi
2025 - IRG/WP 25-11073
Wood rotting fungi play a crucial role in biodeterioration of wood. Many brown-rot fungi are known to rapidly degrade cellulose in wood despite lacking enzymes with cellulose-binding domains. This has led to the hypothesis that they rely on a non-enzymatic degradation system. We recently discovered a novel cellulose-binding domain, CBM104, in the brown-rot fungus Gloeophyllum trabeum. In this stud...
Y Kojima, N Sunagawa, M Aoki, S Tagawa, M Wada, K Igarashi, M Yoshida
Investigation of oxalic acid production in brown-rot fungi
2025 - IRG/WP 25-11074
Brown-rot fungi play a crucial role as wood decomposers in forests and are also known as the main cause of biodeterioration of wooden buildings in Japan. Therefore, understanding their decomposition mechanisms is important for the maintenance and preservation of wooden structures. While white-rot fungi generally use enzyme-system for degradation, many brown-rot fungi lack cellobiohydrolases which ...
S Matsumoto, R Tsukida, T Ito, M Iritani, M Yoshida
Interaction between AA9 lytic polysaccharide monooxygenase and PQQ-dependent pyranose dehydrogenase in cellulose degradation
2025 - IRG/WP 25-11075
Wood-decaying fungi degrade cellulose, the primary polysaccharide in wood, as a nutrient source by utilising various hydrolytic and oxidative enzymes. Among these, lytic polysaccharide monooxygenase (LPMO9) has gained attention as a key enzyme that enhances the degradation efficiency of other cellulases. The activity of LPMO9 requires both electrons and hydrogen peroxide. It has been reported that...
H Murai, Y Kojima, E G Kommedal, V G H Eijsink, M Yoshida
Functional Analysis of Cellobiose Dehydrogenase from the Saprotrophic Basidiomycete Coprinopsis cinerea
2025 - IRG/WP 25-11076
The plant saprophytic fungi including wood rotting fungi secrete various extracellular hydrolytic and oxidative enzymes to degrade wood cell wall. Among these, cellobiose dehydrogenase (CDH) is known as a flavoheme enzyme composed of a flavin domain with FAD and a b-type heme domain. CDH oxidises the reducing end of cellobiose, and transfers electrons to redox partners such as lytic polysaccharide...
Y Sakka, A Hosaka, M Yoshida
Effect of the Cellulose-Binding Domain Associated with Xylanase on the Degradation of Softwood and Hardwood Xylan
2025 - IRG/WP 25-11078
Brown rot fungi rapidly degrade hemicellulose, which is recognized as a key decomposition process during the early stages of wood decay. Brown rot fungi possess multiple genes encoding hemicellulose-degrading enzymes, suggesting that enzymatic hydrolysis plays an important role in this process. Hemicellulases produced by these fungi often contain an additional domain classified as carbohydrate-bin...
R Tsukida, Y Kojima, S Kaneko, M Yoshida