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Immunolocalization of extracellular metabolites from Tyromyces palustris
1991 - IRG/WP 1491
Polyclonal antisera produced to extracellular metabolites from the brown-rot fungus Tyromyces palustris was used in immunogold TEM studies. Gold labelling was detected in the fungal cell wall and extracellular slime layer but little or sporadic labelling was noted within the cytoplasm of the fungal hyphae. Gold particles were also found within the wood cell wall of Pinus densiflora decayed by Tyro...
Yoon Soo Kim


Immunolocalization of xylanase produced by Clostridium xylanolyticum Rogers and Baecker during anaerobic decay of Pinus patula
1991 - IRG/WP 1505
The xylanolytic fraction from a 72h culture of Clostridium xylanolyticum ROGERS and BAECKER was separated using non-denaturing PAGE and then used to raise polyvalent antisera in rabbits. Antibodies were purified using an affinity gel column and coniugated with colloidal gold. Pinus patula was exposed to axenic cultures of Clostridium xylanolyticum for 3 weeks, fixed, embedded and converted to ultr...
A A W Baecker, G M Rogers


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson