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A technique for fructification and basidiospore production by Serpula lacrymans (Schum. ex fr.) SF Gray in artificial culture
1986 - IRG/WP 2255
A method for fructification and basidiospore production by Serpula lacrymans is outlined. A wheat flour/malt extract medium or an oat grain medium, enriched with alanine, tri-potassium phosphate and thiamine supported good fructification of the fungus at a temperature of 12°C. The influence on fructification of temperature, light and inoculum is discussed. To date, 22 stains from a total of 38 te...
S Cymorek, B M Hegarty


Lethal temperature for some wood-destroying fungi with respect to eradication by heat treatment
1984 - IRG/WP 1229
The lethal heat dosis as a function of temperature and time of exposure has been tested for mycelium and for wood infested by three strains of Serpula lacrymans and by Coniophora puteana, Poria monticola, Paxillus panuoides, Gloeophyllum trabeum and Gloeophyllum sepiarium. Even for Serpula lacrymans, which is well known for its sensivity to heat treatment, 30 min at 55°C were necessary to kill th...
M Miric, H Willeitner


Bestimmung der pilzwidrigen Wirksamkeit von Holzschutzmitteln gegen Moderfäule-Erreger
1977 - IRG/WP 2125
Ziel der vorliegenden Arbeit war der Vergleich der in verschiedenen Ländern zur Bestimmung der pilzwidrigen Wirksamkeit von Holzschutzmitteln gegen Moderfäule-Erreger angewandten Methoden. Als Vergleichsmaterial dienten drei normierte Methoden: die britische (42), skandinavische (45) und die in der DDR angewendete (47) sowie wichtige, von verschiedenen Fachleuten ausgeführte Arbeiten experiment...
J Wazny


Comparative study of the degradative ability of autochthonous and standardized strains of wood decay fungi
1996 - IRG/WP 96-10178
This study is intended to assess the biomass, the extracellular protein production, and the carbohydrate degradating ability of three standarized (Chaetomium globosum, Trametes versicolor and Gloeophyllum trabeum) and six autochthonous wood decay fungi strains, with unknown degradating effects. The carbohydrate sources used were those that are usually found in wood cells (glucose, galactose, manos...
F Llinares, D Muñoz-Mingarro, J E Garcia de los Rios, M T De Troya, M Yuste, F Rubio, F Alvarez, P Jiménez, C Rodríguez-Borrajo, A M Navarrete


Old and new facts on the dry rot fungus Serpula lacrymans
1991 - IRG/WP 1470
The article collates some of the recent literature on the biology of the dry rot fungus Serpula lacrymans. The fungus can grow at 28°C, and maximum wood moisture is above 55%. Serpula Iacrymans degrades crystalline cellulose. The intensive production of extracellular oxalic acid is neutralized by calcium and iron. There is considerable variation among the strains with regard to factors such as gr...
O Schmidt, U Moreth-Kebernik


Variations in the virulence of test strains of Coniophora puteana (Schum ex Fr) Karst
1982 - IRG/WP 2185
In laboratory experiments using petri-dishes and small wood blocks (30 x 10 x 5 mm³) 6 different isolates of Coniophora puteana strain BAM 15 were compared with strain FPRL 11E for growth rate and decay capability. Only the recently received strain from EMPA was suitably active giving 24% weight loss after 6 weeks compared with 38.1% for 11E. Collation of data from standard test records revealed ...
A F Bravery, J K Carey, W Worley


Application of DNA fingerprinting methods to identify biocontrol strains of fungi imperfecti
1994 - IRG/WP 94-10068
We have analyzed a number of biocontrol strains of Trichoderma harzianum and other Trichoderma strains with the methods DNA fingerprinting and PCR fingerprinting to differentiate and identify these strain which is not possible with morphological or biochemical methods. We could differentiate even gamma-ray induced mutants from each other as well as different strains form the same and different spe...
A Schlick, K Kuhls, W Meyer, E Lieckfeld, T Börner, K Messner


Characterization of Poria indoor brown-rot fungi
1995 - IRG/WP 95-10094
The heterogeneous group of "Poria" fungi causing brown rot in buildings and also of wood in ground contact comprises Antrodia vaillantii, Antrodia serialis, Antrodia sinuosa, Antrodia xantha and Tyromyces placenta. These fungi have similar morphological appearance and biology. Their nomenclature has a confusing history and is still not uniform. As a consequence, misinterpretations may occur. SDS p...
O Schmidt


On the species problem in Nicobium castaneum (Col., Anobiidae)
1975 - IRG/WP 134
Nicobium castaneum (Oliv.) is one of the most important wood and book worms of the Mediterranean area (eg Portugal) and South Russian. It also occurs in the USA and Japan. A previous paper (Cymorek 1972) left open the question: is Nicobium castaneum one or two species? Nicobium castaneum is currently regarded as forming two varieties or sub-species - sub-specific taxa which are rarely well defined...
S Cymorek


Aureobasidium or Hormonema? A Genetic Approach.
2004 - IRG/WP 04-10529
Aureobasidium pullulans is the main organism causing disfigurement of coatings on wood and the surface of exposed timber. This disfigurement of timber in-service is referred to as “bluestain in-service”. A. pullulans is also associated with the sapstaining of dead wood in the forest and in-service. A. pullulans is noted for its highly variable growth forms (polymorphisms). This variability pre...
M J Ray, D J Dickinson, M Buck


The effect of added nutrients on growth rate and decay capacity of Serpula lacrymans
1990 - IRG/WP 1427
At the previous meeting a new technique was presented that enables both fungal growth rate and wood decay rate to be measured using the same timber specimen. The technique (IRG/WP/1384) has previously been carried out with 1% malt as the sole nutrient within the small jar that provides the inoculum for this method. Results presented here relate to an additional level of 5% malt, with or without a ...
J D Thornton, A McConalogue


A comparative analysis of Coniophora olivacea (Fr. ex Pers.) Karst. and Coniophora puteana (Schum. ex Fr.) Karst. test strains
1993 - IRG/WP 93-20004
Investigations were carried out to compare pure cultures of Coniophora olivacea (Fr. ex Pers.) Karst. used as a test fungus in Australia and other Pacific countries, and Coniophora puteana (Shum. ex Fr.) Karst. which is used in Europe. Comparisons included morphology, growth rate and dry mass of mycelium, decay capacity, influence of temperature, toxic value of CCA and quaternary ammonium compound...
J Wazny, L J Cookson


Physiological properties of fungal test strains according to the European Standard EN 113
1986 - IRG/WP 2258
For the discussion of the European standard EN 113 the EMPA's procedure of culturing the test fungi and the corresponding virulence of the test fungi as well as the wood moisture content at the end of the test are shown. It is mainly shown that within the standard the choice of the solvent may not be left at the test lab if reproducible results shall be obtained. The different solvents in...
E Graf, B Zgraggen, P Manser


Decay potential of various New Zealand Phlebiopsis gigantea strains using a soil block method
1999 - IRG/WP 99-10309
The objective of the current study was to determine the decay potential of different strains of Phlebiopsis gigantea using Pinus radiata and European Fagus sylvatica, respectively. A soil block method was used to determine mass weight loss of P. radiata and F. sylvatica following 10 weeks incubation with different strains of P. gigantea. For comparison, Trametes versicolor, Postia placenta, Gloeop...
B Kreber, J G Van der Waals


A comparison of fungal strains used in the bioassay of wood preservatives
1984 - IRG/WP 2220
Previously published data are presented relating to a number of strains of wood-destroying basidiomycetes (Coniophora puteana, Coriolus versicolor, Gloeophyllum abietinum, Gloeophyllum sepiarium, Gloeophyllum trabeum, Lentinus lepideus, Poria placenta, Fibroporia vaillantii and Serpula lacrymans) commonly used as test fungi in the bioassay of wood preservatives. The data, which has not been statis...
J Wazny, H Greaves


Screening of fungal strains for wood extractive degradation
1998 - IRG/WP 98-10254
Fungal strains were screened for their ability to degrade apolar extractives in wood from scots pine. The degradation of total wood extractives by 91 different strains was monitored in stationary batch assays incubated for 6 weeks. The results obtained show that the ability of wood-inhabiting fungi to utilize wood extractives varied greatly, even for different isolates of the same species. Fungal ...
J Dorado, M J Martinez-Inigo, T A van Beek, F W Claassen, J B P A Wijnberg, R Sierra-Alvarez


List of fungi in soft-rot tests
1975 - IRG/WP 105
This list gives information on the terrestrial and marine species and strains of fungi tested for soft-rot ability in pure culture with data on their decay activity in untreated wood. An index of the timber species tested is added. The compilation is based on 1) the list of soft-rot fungi of the former OECD Group (Doc.No. 27/DAS/CSI/M/559), Oct. 1968, prepared by R. Rösch and W. Liese, 2) informa...
G Seehann, W Liese, B Kess


Lindane and permethrin tolerances of different strains of Lyctus brunneus Steph
1986 - IRG/WP 1278
The question about the reproducibility of test data from contact insecticide tests with Lyctus brunneus has been asked within the IRG. Three laboratories decided to interchange their insects and to carry out a comparative test according to the EMPA screening method. In order to get enough animals for the test the three strains had to be grown during three generations in each of the laboratories. D...
E Graf, B Lanz


Characterization and differentiation of wood rotting fungi by protein and enzyme patterns
1999 - IRG/WP 99-20177
Standardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several auth...
U Schoknecht


A comparison analysis of eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray
1991 - IRG/WP 2362
Investigations were previously carried out to compare eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray (some used in various countries as standard test strains): FPRL 12C (England), FPRL 12E (Germany), Warsaw III (Poland), HFP 7802 (Japan), DFP 16508, 16509, 16521 and 16522 (Australia). Studies included growth rate and dry mass of mycelium, decay capacity, reduction of compression stre...
J Wazny, J D Thornton


Variation in two Postia placenta strains, MAD-698-R and FPRL 280 – mass loss, DNA content and gene
2012 - IRG/WP 12-10781
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and brown rot fungi are also largely responsible for the destructive decay of wooden structures. The aim of this study was to compare two commonly used strains of Postia placenta – MAD-698-R and FPRL 280. Scots pine sapwood samples were exposed for two and eight weeks to both fungal strains. The following was inv...
N Thaler, G Alfredsen, C G Fossdal


Virulence of two Laboratory Test Strains and one Natural Isolate of Rhodonia (Oligoporus) placenta against Thermally Modified Pine and Beech Wood
2013 - IRG/WP 13-20524
Rhodonia (Oligoporus) placenta is known as a relative aggressive basidiomycete fungus against thermally modified timber. In durability lab tests, this often results in a lower durability class, compared to other test fungi. In the reported work, the virulence of three different strains of Rhodonia placenta for untreated and thermally modified timber was determined and compared. Therefore, two lab ...
K Plaschkies, W Scheiding, K Jacobs, N Rangno


Three-Dimensional Digital Image Correlation of Strains in Profiled Wood Decking Exposed to Wetting & Drying
2017 - IRG/WP 17-20611
We use three dimensional digital image correlation to measure the strains that develop at the surface of profiled radiata pine (Pinus radiata D. Don) deck boards subjected to a wetting and drying cycle. We hypothesise that surface profiling will change the pattern of strain distribution at the surface of deck boards by concentrating stresses at the base of profile grooves. Five groups of three deck board samples were produced from five different pieces of machine stress-graded radiata pine wood. Two wavy profiles, ribble or ripple, were tested. Flat unprofiled boards acted a control. Each board was fixed to a rigid frame and subjected to a wetting and drying cycle. Full field surface strain data was collected using 3D digital image correlation. Strains varied across the surface of both flat and profiled boards. Profiling changed surface strain patterns; strain maxima and minima developed in the profile ridges and grooves during wetting, respectively, but this pattern of strains reversed during drying. Such a pronounced reversal of strains was not observed when flat boards were exposed to wetting and drying, although there was a shift towards negative strains when flat boards were dried. We conclude that profiling changes surface strain distribution in deck boards exposed to wetting and drying, and causes high strains to develop in the grooves of profiled boards. These findings help explain why checks in profiled deck boards are mainly confined to profile grooves where they are difficult to see, and the success of profiling at reducing the negative effects of checking on the appearance of wood decking.
J Mallet, S Kalyanasundaram, P D Evansa


Biodegradation of creosote by white rot fungus Phanerochaete chrysosporium and a strains of Pseudomonas
2017 - IRG/WP 17-50329
The aim of this study was to determine degradation effectiveness of polycyclic aromatic hydrocarbons (PAH) contained in creosote by white rot fungus and bacteria. The following two species of bacteria Pseudomonas putida and Pseudomonas sp. as well as Phanerochaete chrysosporium fungus characterised by the ability to decompose aromatic compounds were selected for experiments. Bacterial strains were...
J Zabielska-Matejuk, A Stangierska, A Kropacz, E Kaczorek


The Impact of Cryopreservation Techniques and of Water Quality on the Wood Degrading Ability of Fungal Strains: Recommendations for Maintenance and Culturing Procedures
2018 - IRG/WP 18-20636
In order to establish an optimal cryopreservation method, strains of Basidiomycete and Ascomycete fungi, which are among the most frequently used for wood durability testing, were evaluated using three cryopreservation procedures at -80°C. The main objective was to prevent decrease of the fungal virulence that may occur due to repeated routine sub-culturing procedures and to optimize the reactiva...
A Stum, M Montibus, I le Bayon, M Kutnik


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