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Characterisation of growth and stain of different groups of sapstain fungi on lodgepole pine
1999 - IRG/WP 99-10326
Canada is the world's largest exporter of softwood lumber. These softwood shipments are susceptible to a variety of wood-inhabiting fungi that can lead to sapstain discolouration, which in turn decrease the product value. Furthermore, the presence of these microorganisms may be unacceptable to the importing countries. The objective of this work is to assess the sapstaining capability and ...
C Fleet, C Breuil, A Uzunovic, A Byrne


Integrated protection of freshly sawn lumber using Bacillus subtilis and selected fungicide
1997 - IRG/WP 97-10235
Bioprotection against stain fungi has tremendous potential for reducing discoloration of freshly sawn wood while decreasing chemical consumption. Unfortunately, most bioprotectants appear to be unable to consistently perform under the array of conditions to which freshly sawn wood is exposed. While research is underway to understand the nature of the inconsistent performance, a more pragmatic appr...
M E Mankowski, M Anderson, J J Morrell


Variable tolerance of Ophiostoma spp. and Diplodia pinea to commercial antisapstain products
1998 - IRG/WP 98-10266
A recent survey of the occurrence of sapstain fungi in New Zealand, conducted at The University of Waikato, provided the opportunity to examine fungicidal tolerance amongst new isolates. It also enabled a comparison of tolerance amongst new isolates with those used in routine antisapstain screening trials at Forest Research. A rapid antisapstain laboratory disc trial was used to determine the ext...
D R Eden, C M Chittenden, B Kreber, J G Van der Waals, R N Wakeling, R L Farrell, T Harrington


Molecular phylogeny of Ophiostoma spp. related to blue-stain in pine
2002 - IRG/WP 02-10432
Blue-stain in coniferous woods is an economically important disease in many temperate countries, caused specially by genus Ophiostoma. Classical taxonomy of genus Ophiostoma is limited for the correct identification of many species, because of the similar macroscopic and microscopic characters, as well as the existence of some species complex. Molecular identification is a good complement to eluci...
M Villarreal, F Arenal, J Sánchez-Ballesteros, M T De Troya, F Llinares, V Rubio, A Navarrete


Which fungi cause sapstain in Canadian softwoods?
1998 - IRG/WP 98-10285
The Canadian forest products industry suffers considerable losses in revenue due to fungal stains. There is an increased awareness that more complete knowledge about the causal organisms might help solve the problem. A first step was to initiate a thorough survey of bluestain fungi in Canada. Systematic sampling was done at seven selected sawmills in six Canadian provinces. In summer 1997 fresh lo...
A Uzunovic, Dian-Qing Yang, P Gagné, C Breuil, L Bernier, A Byrne


Durability of larch (Larix spp.) wood against brown-rot fungi
1997 - IRG/WP 97-10228
Durability of the heartwood of Larix decidua, L. sibirica, L. gmelinii, L. gmelinii var japonica, L. gmelinii var olgensis and L. sibirica x decidua against brown rot fungi Coniophora puteana, Poria placenta and Gloeophyllum trabeum was tested according to EN 113 test method. Parallel samples were used to study the amount and composition of wood extractives. The sample trees originated from the re...
H Viitanen, L Paajanen, P Saranpää, P Viitaniemi


Chemical compounds from Eperua falcata and Eperua grandiflora heartwood and their biological activities against wood destroying fungus (Coriolus versicolor)
2005 - IRG/WP 05-30373
The chemistry analysis of the compounds present in dichloromethane and ethanolic fraction as well as bioassays enables to understand the durability differences of Eperua falcata and Eperua grandiflora. The principal distinction between these two species is the acidic subfraction of diterpenoic extract, which is antifungic in Eperua falcata when tested in in-vitro conditions. This study also enable...
N Amusant, C Moretti, B Richard, E Prost, J M Nuzillard, M-F Thévenon


An investigation concerning Camponotus spp. distribution and damage in buildings in Sweden
1985 - IRG/WP 1248
This is a report of an investigation by a Swedish insurance company on the occurrences of damage by Carpenter ants during 1974 to 1981. The distribution of damage in walls, roofs and floors of both permanent homes and summer-houses has been assessed. It is concluded that the increased frequency of attack is becoming economically serious....
V Butovitsch, K-J Hedqvist, C Tornberg


Antagonistic effect of Trichoderma spp. against Serpula lacrymans in the soil treatment test
1991 - IRG/WP 1473
Soil treatment tests for preventing growth of Serpula lacrymans were conducted using Trichoderma spp. as antagonists. Soil specimens tested were Kanuma-soil without organic matter and the horticultural soil which was collected from the test site of the stake test. Perfect efficacy of treatment with Trichoderma spp. was shown when the horticultural soil without sterilization was used as a soil spec...
S Doi, A Yamada


The development of a screening method for the activity of pyrethroids against wood boring marine crustaceans, Limnoria spp
1978 - IRG/WP 443
The present work is concerned with the develepment of a suitable bio-assay technique to determine the biological activity (contact action) of pyrethroids against Limnoria spp. Estimates of the toxicity of three pyrethroids, permethrin, cypermethrin and decamethrin (the structures of which are shown in Fig. 1.) to the marine borer have been obtained....
D Rutherford, R C Reay, M G Ford


Controlling Coptotermes (Isoptera: Rhinotermitidae) infestations in buildings with bait boxes
2000 - IRG/WP 00-10372
This paper describes the results of a commercial pest control operators use of the CSIRO bait box procedure in controlling infestations within buildings of subterranean termites ('termites') of the genus Coptotermes. Polystyrene bait boxes (480 x 330 x 210 mm3) were filled with alternate layers of corrugated cardboard and kiln-dried hardwood strips of Eucalyptus regnans F. Meull....
J R J French, T Boschma


Contribution to study of the degradation caused in Pinus spp. poles used in field test
1989 - IRG/WP 1417
The study of the degradation produced by soil natural microflora on wood in contact with it in the field, has been going on for several years now. Our contribution to this aim in the present work has dealt with the possible relationship of the microorganisms in the soil. The microscopic visualization of wood colonization by the microorganisms, and the chemical analysis of the degraded wood compare...
M T De Troya, A Garcia, M J Pozuelo, A M Navarrete, A Cabanas


Targeting fungal proteinases to prevent sapstain on wood
1995 - IRG/WP 95-10097
Discoloration of wood, caused by a variety of sapstaining fungi, leads to periodic losses in the Canadian lumber export industry. Proteolytic enzymes are thought to be necessary for retrieval of nitrogen during fungal growth on wood. The major extracellular proteinase of Ophiostoma piceae, a representative sapstaining fungus, was purified to homogeneity and its inhibition pattern characterised. Cl...
L D Abraham, D E Bradshaw, A Byrne, P I Morris, C Breuil


Biological control of Serpula lacrymans using Trichoderma spp
1994 - IRG/WP 94-10069
The effectiveness, or otherwise, in killing Serpula lacrymans, of a range of Trichoderma spp. in a variety of media and using two different incubation systems has been tested. In agar based systems with normal nutrients or minimal nutrients with high or low nitrogen contents and high or low iron content Trichoderma harzianum 25 proved to be the most efficient and killing Serpula lacrymans. Other s...
A J Score, J W Palfreyman


Blue-stain fungi (Ceratocystis spp.) found in Spain on pine woods
1989 - IRG/WP 1410
So far, there is only a very limited reported description of the different Ceratocystis spp. present on fresh wood in Spain. So, the main goal of this work has been the identification of species of this genus causing blue-stain on Pinus pinaster A. Ait. and Pinus sylvestris L. woods. We have also investigated the relationship between the species found and their propagation vectors (insects and win...
M T De Troya, A M Navarrete


Effect of methylene bisthiocyanate on morphology and ultra-structure of a sapstain fungus, Ophiostoma floccosum
2003 - IRG/WP 03-10471
In vitro effects of methylene bisthiocyanate (MBT) on hyphal morphology and ultrastructure of Ophiostoma floccosum were examined using differential interference contrast, epifluorescence and transmission electron microscopy (TEM). Differential interference contrast microscopy suggested that MBT caused rapid changes to the morphology of O. floccosum resulting in excessive formation of vacuoles and...
T Singh, B Kreber, R N Wakeling, A Stewart


Detection of semi-quantitative and qualitative enzymatic activities of blue-stain fungi
2000 - IRG/WP 00-10347
Blue-stain, produced in forests, continues to be a problem in countries of moderate climate. In forestry, in certain geographical areas of Spain, it has been observed that anti-sapstain products have not always been efficient, as their fungicidal effectiveness varies on occasion, depending on the species of wood and microbiota that exist in the region. It is not always easy to identify the species...
M T De Troya, F Llinares, D Muñoz-Mingarro, M J Pozuelo, N Acero, C Rodríguez-Borrajo, A M Navarrete


Variation in Canadian bluestain fungi: Tolerance to DDAC and DOT
1999 - IRG/WP 99-10303
Bluestain in Canadian wood products results in significant and unpredictable losses each year. In order to develop rational methods to eliminate or reduce the sapstain problem, a more complete knowledge of the causal organisms must be gained. This includes a knowledge of the variability in tolerance of different fungal species and strains to commercially used chemicals. In British Columbia, the ma...
J Dubois, A Byrne, J E Clark, A Uzunovic


The leachability and specificity of the biological protection of timber using Scytalidium sp. and Trichoderma spp
1986 - IRG/WP 1302
The results of field experiments, using biological control against internal decay of creosoted poles, are briefly reviewed and the evidence concerning the leachability of the antibiotics produced by these species is presented. A pure culture miniblock decay test on biological control treated pine sapwood is described and the results compared to previously published data. The protection against Len...
P I Morris, N A Summers, D J Dickinson


The use of pyrethroids against Limnoria spp
1981 - IRG/WP 473
Synthetic pyrethroid insecticides discovered in the last fifteen years have been developed as crop sprays and used in aerosol formulations for the control of household pests. High toxicity to a wide range of arthropod pests, combined with reasonably low mammalian toxicity, confers an advantage over many other control agents. Some of the more recent pyrethroids are photostable and display residual ...
D Rutherford, R C Reay, M G Ford


Copper-resistant fungi on pressure impregnated wood in Denmark
1994 - IRG/WP 94-10078
The occurence of Amorphotheca resinae Parbery and its asexual stage Cladosporium resinae (Lindau) de Vries on CCA and CCB treated wood has previously been shown. In the autumn 1993 some other blue stain fungi were found on CCP and CCB treated pine timber, such as Ophiostoma minus (Hedgc.) H. and P. Sydow, Ophiostoma pilifera (Fr.) H. and P. Sydow and Ophiostona piceae (Munch) H. and P. Sydow. The ...
J Bech-Andersen, S A Elborne


The use of immunofluorescence labelling for detecting Ophiostoma piceae in radiata pine
1998 - IRG/WP 98-10268
The primary objective of the current investigation is to understand fungal interactions of dominant sapstaining fungi in radiata pine using confocal laser scanning microscopy (CLSM). In the current paper, an immunofluorescence labelling technique was developed for detection of Ophiostoma piceae using a monoclonal antibody. The primary antibody was labelled with Oregon green 514 Goat anti-mouse Ig...
Ying Xiao, B Kreber, C Breuil


Biological control of sapstain fungi in wood
1996 - IRG/WP 96-10158
Sapstain fungi can cause serious damage to wood and wood products, resulting in a significant economic loss for the wood products industry. The purpose of the present investigation was to determine whether the sapstain in wood caused by sapstain fungi could be biologically controlled. Biological control of sapstain fungi in wood was demonstrated in field trials with nonpigmented isolates of Cerato...
S C Croan


Effect of media composition on the antagonistic properties of Trichoderma spp. against wood decay fung
1992 - IRG/WP 92-1538
Most screening of potential biocontrol agents of wood decay fungi has previously been undertaken using artificial media. Similarly experiments designed to evaluate the mechanisms involved in antagonism between biological control agents and target fungi, have largely been carried out in conditions which do not accurately reflect the nutrient status of wood. This paper examines the influence of nutr...
U Srinivasan, A Bruce, H J Staines


Using DNA probes to characterize the metabolic pathway of pigment production in several wood-staining fungi
1996 - IRG/WP 96-10146
During shipment and storage, lumber is susceptible to sapstain, a wood discoloration caused by fungi. Currently kiln drying and chemical applications are used to control sapstain. However, the chemicals used to protect wood have a broad range of action, and so can affect other organisms. In addition, in Canada most of these chemicals are under temporary registration. Thus there is a need to develo...
R Eagen, S Riecken, J Kronstad, C Breuil


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