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rDNA-ITS sequence of Serpula lacrymans and other important indoor rot fungi and taxon-specific priming PCR for their detection
1999 - IRG/WP 99-10298
Taxon-specific priming polymerase chain reaction (TSPP) is a powerful molecular tool for fungal diagnosis. For its application to indoor rot fungi, the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) of the main fungal species causing wood rot in European buildings was amplified with the polymerase chain reaction (PCR). The ITS region was sequenced. The complete sequences are present...
O Schmidt, U Moreth


Data sheet on wood-boring insects. Apate monachus Fabricius. 2. Position systématique, nomenclature, identification et distribution - Espèces végétales attaquée
1981 - IRG/WP 1105
R L A Damoiseau


Penichroa fasciata (Stephens) (Col. Cerambycidae) a pest in wood materials
1988 - IRG/WP 1365
Penichroa fasciata (Stephens) (Col. Cerambycidae) is found to be a frequent pest occurring in hardwood in storage in Italy. This paper reports the characteristic for identification, biological features, distribution and timber liable to attack....
A Gambetta, E Orlandi.


A rapid field bioassay technique with subterranean termites
1983 - IRG/WP 1188
Details are summarised of a field procedure which is designed to ensure continuous exposure to a replenishing termite biomass. After pre-baiting to determine the presence and identification of a termite hazard, test specimens (35 x 35 x 250 mm³) are installed vertically in the ground adjacent to and in contact with bait specimens of the same dimensions and interconnected by susceptible feeder str...
C D Howick, J W Creffield


The identification and preservative tolerance of species aggregates of Trichoderma isolated from freshly felled timber
1992 - IRG/WP 92-1553
The surface disfigurement of antisapstain treated timber by preservative-tolerant fungi remains a major problem in stored timber. Identification of a range of isolates of Trichoderma based on microscopic morphological characteristics was found to be imprecise due to the variable nature of this organism. In addition, studies to compare visual (morphological) characteristics of these isolates with t...
R J Wallace, R A Eaton, M A Carter, G R Williams


What can DNA fingerprinting, aggression tests and morphometry contribute to the identification of colonies of the Formosan subterranean termite
2000 - IRG/WP 00-10371
Multilocus DNA fingerprinting, aggression tests and morphometry were compared to evaluate their potential for the identification of colonies of the Formosan subterranean termite, Coptotermes formosanus (Isoptera: Rhinotermitidae) in Hawaii. DNA fingerprinting separates the termites from all studied collection sites. Since the genetic similarity between termites from different collection sites lies...
C Husseneder, J K Grace


On the problem "House Longhorn Beetle" in hardwoods and an aid to distinguish between Hylotrypes bajulus and Hesperophanes cinereus larvae
1981 - IRG/WP 1141
S Cymorek


Rapid leaching test
1991 - IRG/WP 2367
An accelerated test which is suitable for measuring the extent of metal fixation in both chromium and non chromium containing preservatives is described....
J A Cornfield, M Bacon, A Lyman, C Waldie, M R Gayles


Data sheet on wood-boring insects: Ptilinus pectinicornis (Linnaeus)
1987 - IRG/WP 1334
S Cymorek, M-M Serment


Isolation and identification of the fungal flora in treated wood
1976 - IRG/WP 144
J F Levy


Identification card for Coriolopsis polyzona (Pers.) Ryv
1977 - IRG/WP 161
A Ofosu-Asiedu


The use of C CP/MAS NMR in the chemical identification of decayed and undecayed, tropical timber species
1984 - IRG/WP 1224
13C CP/MAS NMR was found to be an extremely powerful tool for elucidating the chemical composition of Eucalyptus maculata, Pinus elliottii and Alstonia scholaris. The differences in lignin composition were different for each timber and discussed in relation to decay caused by soft-rot and white rot fungi. In particular the presence of syringyl and guaiacyl lignin types are discussed.
L E Leightley


Isolation and identification of bacteria from degraded wood - A progress report
1986 - IRG/WP 192
During attempts to isolate tunnelling bacteria in pure culture, physical separation from other bacteria was successfully achieved by the use of cellophane. Cellophane was used in liquid cultures as a substrate for tunnelling bacteria that were present in a mixture with other bacteria. By tunnelling into cellophane, TB were physically removed from contaminating bacteria which could then be removed ...
J A Drysdale, P J Ruland, J A Butcher


Isolation and identification of bacteria from CCA-treated eucalypt power transmission poles
1987 - IRG/WP 1317
Bacteria have been isolated from CCA treated Eucalyptus maculata poles which possessed characteristic bacterial decay patterns. Cultures were isolated on a number of different media which had been inoculated with a splinter of degraded wood. Primary tests indicated that most isolates belonged to the genera Pseudomonas and Bacillus. The species Pseudomonas cepacia was most frequently isolated....
G A Willoughby, A C Hayward, L E Leightley


Bioassays for rapid assessment of heavy metal toxicity in seawater
1998 - IRG/WP 98-50112
In evaluating the effect of CCA leachate on the establishment of fouling communities on wood exposed in the sea, three organisms were assessed as biological indicators of heavy metal toxicity. These were two macroalgae, Fucus serratus and Undaria pinnatifida. and the motile protist thraustochytrid, Schizochytrium aggregatum. The choice of these organisms for the tests carried out was based on the ...
C J Brown, R L Fletcher, R A Eaton


The dry rot fungus Serpula lacrymans, its growth and damaging of wood
2004 - IRG/WP 04-10511
The dry rot fungus (Serpula lacrymans) is one of the most dangerous wood rotting fungi, especially in the built environment. In our mycological laboratory more experiments with this fungus have been carried out aimed at:- its growth under stable and variable climatic conditions, - its possibility to attack either natural wood of different species and also modified wood primary pre-treated with ...
L Reinprecht


A rapid colorimetric assay for mold spore germination using XTT tetrazolium salt
2011 - IRG/WP 11-20462
A rapid colorimetric assay was developed to quantitate metabolic activity in mold spores during germination using 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[9phenyl-amino)carbonyl]-2H-tetrazolium hydroxide (XTT). The assay was used to demonstrate inhibition of spore germination following exposure to different biocides and variability in the inhibition of spore germination of different mold specie...
C A Clausen, V W Yang


Rapid analytical methods for wood waste - An overview
1998 - IRG/WP 98-50104
The proper handling of wood after service is a today's problem. Untreated wood could be reused or may be used as fuel. For treated wood special care is demanded to avoid environmental impacts. Thus, analytical methods are requested to detect rapidly whether and to what extend wood is contaminated, covering a wide spectrum of organic and inorganic agents used during the last 50 years. Trad...
A Peylo, R-D Peek


Identification of terminal structures in cellulose degraded by the brown-rot fungus Postia placenta
1989 - IRG/WP 1389
To gain insight into the biochemical mechanism employed by brown-rot fungi to depolymerize cellulose, we identified the end-groups of chemically pure cellulose that had been depolymerized by the brown-rot fungus, Postia placenta. The depolymerized cellulose was acid hydrolyzed and the anion fractions isolated by ion chromatography. Sugar acids were identified by gas chromatographic and mass spectr...
T K Kirk, T L Highley, R E Ibach, M D Mozuch


Occurrence, prevention and repair of Dry Rot
1990 - IRG/WP 1439
Information about the frequency, detection, identification and repair of Dry Rot attacks (Serpula lacrymans) is summarized from 13 European countries. Based on 28 completed and returned questionnaires it appears that Dry Rot attacks are recognized in all countries participating, and that there is an appreciable similarity in the frequency of attacks and methods of detection and identification. How...
A P Koch


Identification of phanerosporic acid in birch degraded by Phanerochaete chrysosporium
2003 - IRG/WP 03-10496
Extracts of Phanerochaete chrysosporium cultures grown on birch or on a malt extract-peptone-glucose agar medium were analysed by HPLC. A major component from the two sources appears to be identical by HPLC and UV-visible spectrometry. The product isolated from agar-grown cultures was purified to apparent homogeneity and structure analysis by NMR indicates that the metabolite is the beta-resorc...
M D Mozuch, P J Kersten


Rapid analysis - chances and limitations
1999 - IRG/WP 99-50130
The reuse of wood out of service in the particle board industry demands a proper handling and separation of assortments with differing content and nature of preservative. A pre-selection based on visual and olfactorial characteristics can be carried out for certain assortments like sleepers, poles, etc. Problems arise from diffuse and less intensive treated wood which is regularly dip-treated or b...
A Peylo, R-D Peek


A rapid method for collecting large numbers of subterranean termites from wood
1982 - IRG/WP 1165
A corrugated fiberboard trap is described which attracts large numbers of subterranean termites from infested logs. Single-trap collections in excess of 63 grams (wet wt.) for Reticulitermes virginicus (Banks) and 43 grams for Coptotermes formosanus Shiraki were taken one to three days following discovery by termites. If traps are removed from the source logs shortly after beeing attacked, termite...
J P La Fage, N-Y Su, M Jones, T Lawrence


Molecular studies on house rot fungi by RAPD-PCR
1997 - IRG/WP 97-10195
For genetic information and in view as a possible diagnosis method various isolates of the dry rot fungus, Serpula lacrymans, the wild merulius, S. himantioides, and the cellar fungus, Coniophora puteana, from Asia, Europe and USA were investigated by the polymerase chain reaction (PCR) based technique of randomly amplified polymorphic DNA (RAPD). The banding patterns obtained revealed for S. lacr...
O Schmidt, U Moreth


A technique for the rapid assessment of wood surface degradation during weathering
1987 - IRG/WP 2281
Radiate pine (Pinus radiata D. Don) sapwood samples taken from four trees were converted into thin strips 100 x 10 x 0.085 (85 µm) mm³, (longitudinal x radial x tangential) in size and exposed in preweighed hatches to the weather inclined at 45° facing equatorially for 10, 20, 40, 60, 80 and 100 days. Significant (P < 0.001) weight losses occurred with time over the exposure period. Differen...
P D Evans, D Abbott, S Thein


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