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Investigation of extracellular mucilaginous material in some wood decay fungi
1996 - IRG/WP 96-10188
The external morphology of the extracellular mucilagenous material (ECM) produced by Coriolus versicolor and Coniophora puteana during colonization of Scots pine and beech was studies using SEM. Specimens were examined in the frozen hydrated, freeze-dried and critical point dried state. All technics produced artefacts but the ECM was best preserved when examined the frozen hydated state. Critical ...
A R Abu, D J Dickinson, R J Murphy


Dura-Treet II, a water dispersible pentachlorophenol
1980 - IRG/WP 3165
During the past several decades, penta petroleum wood treating has become increasingly popular. Penta is an effective chemical compound against wood organisms causing decay and rot, is safe to handle, also clean and easy to use. In terms of preservative cost, it has been and remains by far the least expensive of the three major preservatives. However, when the cost of petroleum carrier is added, t...
D B Hatcher


Basidiospore structure and germination of Serpula lacrymans and Coniophora puteana
1988 - IRG/WP 1340
Using a nuclear staining technique and fluorescence microscopy, the basidiospores of Serpula lacrymans were shown to be uninucleate whereas those of Coniophora puteana were binucleate. The germination rate of the Serpula lacrymans spores, which was considerably lower than that of Coniophora puteana, decreased further after storage or heat treatment. Transmission electron micrographs indicate that ...
B M Hegarty, U Schmitt


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


A comparison of analytical and visual techniques used for assessment of weathering properties of chromium and copper azole treated timber
1994 - IRG/WP 94-20023
The weathering properties of preservative treated timber were studied by measuring surface lignin loss and by microscopic examination after weathering, and results were related to qualitative assessments of visual appearance after weathering. Measurement of relative rates of delignification of untreated and preservative treated timber obtained by FTIR analysis, and weight loss determined on thin v...
J A Cornfield, M D C Hale, G Fettis


Preliminary Studies on ZiBOC- A Potential Eco-friendly Wood Preservative
2005 - IRG/WP 05-30372
Over the past decade, the wood processing industry has been increasingly involved in strategies to minimize the environmental impacts of treated wood. One sign of this is the dramatic increase in the use of preservatives based on inorganic metal oxides, which fix in wood. Among such preservatives the most common is chromated-copper-arsenate. In Ascu chromium and arsenic both are carcinogenic thus...
S Tripathi, J K Bagga, V K Jain


Weathering of Wood Modified with the N-Methylol Compound 1,3‑dimethylol-4,5-dihydroxyethyleneurea (DMDHEU)
2009 - IRG/WP 09-40467
Scots pine (Pinus sylvestris L.) sapwood boards were treated with a methylated 1,3‑dimethylol-4,5-dihydroxyethyleneurea (mDMDHEU) causing weight gains of approx. 25% and exposed to weathering for 18 months. Compared to untreated boards, treatment with mDMDHEU reduced surface discoloration mainly caused by staining fungi. Boards treated with mDMDHEU exhibited clearly lower moisture content th...
C Mai, Yanjun Xie, A Krause, K Urban, P D Evans, K Richter, H Militz


The invasion channels of damage fungi in bamboo lumber
2010 - IRG/WP 10-10712
The micro-agents on bamboo lumber damage are mould, stain, and decay fungi. This research is about invasion channels of bamboo lumber damage fungi by scanning electron microscope (SEM). The results showed that the conidia of mould were only accumulated on the surface of bamboo and no hyphae detected in bamboo vascular bundle, and hyphae of stain penetrated into bamboo and grew in metaxylem vessel...
Xingxia Ma, Mingliang Jiang, Daochun Qin


The Use of Micro-Tensile Testing to Assess Weathering Decay and Oxidative Degradation of Wooden Items
2010 - IRG/WP 10-20433
This paper presents a method to study the weathering performance of wooden items and to assess the oxidative degradation of wood via the Fenton reaction. Weathering resistance and photo-stability of wood was tested using pine wood (Pinus sylvestris) veneer strips measuring approximately 60 µm in thickness. The veneer strips were treated using a reactive chemical to impart surface protection. The ...
C Mai, Yanjun Xie, Zefang Xiao, P D Evans, H Militz


Analyzing hyphal growth of the bio incising fungus Physisporinus vitreus with light-, confocal laser scanning- and, Synchrotron X-ray tomographic microscopy
2010 - IRG/WP 10-20438
Norway spruce wood is an important building material for the forest and wood industry. To improve wood permeability and uptake of wood modification substances Physisporinus vitreus is employed as a bioincising agent. In this study wood specimens were incubated with P. vitreus to assess hyphal colonisation and structural alterations of Norway spruce wood. After different incubation periods, semi-th...
C Stührk, M Fuhr, M Schubert, F W M R Schwarze


Copper Nanoparticles in Southern Pine Wood Treated with a Micronised Preservative: Nanodistribution of Copper in the Pit Membrane and Border of an Earlywood Bordered Pit
2011 - IRG/WP 11-30566
Copper nanoparticles can penetrate the cell walls of unlignified parenchyma cells in southern pine wood treated with a micronised wood preservative, but they are excluded from lignified tracheid walls. This paper extends these observations to include the cell wall layers of the bordered pit. Focused ion beam and ion milling were used to make an ultra-thin section of the cell wall layers of an earl...
H Matsunaga, Y Kataoka, M Kiguchi, P Evans


Novel microscopic approaches to visualise chitosan within impregnated wood
2012 - IRG/WP 12-20485
Chitosan, a deacetylated product of an abundant naturally occurring biopolymer chitin, has been used in a range of applications, particularly in food and health areas, as an antimicrobial agent. In the work reported here Pinus radiata wood was impregnated with chitosan as an environmentally compatible organic biocide. It is important to understand micro-distribution of bioprotectants in impregnate...
A Singh, T Singh


Accessibility of Wood Cell Walls to Well-defined Platinum Nanoparticles
2012 - IRG/WP 12-20494
Copper nanoparticles are found in the walls of parenchyma cells in southern pine sapwood treated with a micronised wood preservative, but they are absent from tracheid walls. Hence, we hypothesized that small nanoparticles can penetrate the walls of unlignified parenchyma cells, but are excluded from lignified tracheid walls. This paper tests this hypothesis by treating pine sapwood with an aqueou...
H Matsunaga, Y Kataoka, M Kiguchi, P D Evans


Preliminary evaluation of degradation patterns in wood samples from the Yenikapı shipwrecks
2013 - IRG/WP 13-10798
The uncovering of several shipwrecks during the excavation of the Marmaray Rail Tube Tunnel Project in Istanbul provides an important opportunity to evaluate waterlogged woods buried for centuries. Identification of these woods showed that a variety of species was used in the ships. One shipwreck (a galley) from the Yenikapı Marmaray was examined using electron microscopy. The micrographs rev...
C Köse, A M Taylor


Scanning Transmission X-ray Microscopy as a tool for examining chemical changes in wood cell walls due to enzymatic activity
2014 - IRG/WP 14-20556
As development of techniques for examining enzymatic activity on solid, complex biological materials, is considered to be an important factor in further development of enzyme applications and for understanding the decay of plant materials, this study explores applicability of Scanning Transmission X-ray Microscope (STXM) for determining chemical changes in enzymatically treated samples at the cell...
D Jeremic, R Goacher, R Yan, C Karunakaran, E R Master


Development of a new characterization method to analyse Cypermethrin penetration in wood material by immunolabelling
2015 - IRG/WP 15-30674
The preservative efficacy of organic biocides is strongly related to their capacity of penetration and retention within wood tissues. The specific detection of the pyrethroid insecticide cypermethrin is currently obtained after extraction followed by chemical analysis by chromatography techniques. However visualizing the insecticide molecule within the wood structure requires specific probes toget...
S Tapin-Lingua, K Ruel, J-P Joseleau, D Messaoudi, O Fahy, M Jequel, M Petit-Conil


The Protective Effect of Different Tree Bark Extractives against Decay Fungi
2017 - IRG/WP 17-30707
There are various modification methods which increase the resistance of wooden materials. Due to recent environmental regulations, there is a need for new and environmentally-friendly wood preservatives. In this study, six different tree bark extractives were evaluated for their wood resistance against fungal degradation. For this purpose, the white rot fungi (Tramates versicolour) and brown rot f...
Ö Özgenç, S Durmaz


Electron microscopic detection and chemical analysis of three-lamellar structures in wood-destroying fungi
1984 - IRG/WP 1240
In the course of transmission electron microscopical investigations of pine wood decay by various brown- and white-rot fungi extracellular three-lamellar structures (TLS) formed by the fungi were found in specimens stained with ruthenium red. These structures occured in the lumen of the wood cell surrounding the hypha at the outermost layer of the fungal cell wall. In the course of the investigati...
R Foisner, K Messner, H Stachelberger, M Röhr


A short note on fungal decay in K33-treated poles
1982 - IRG/WP 1169
Soft rot cavities and erosion of the lumen have been found in K33-treated Pinus sylvestris poles from the years 1956-66 by microscopic studies. Poor treatment quality has been proved for some of these poles. The microscopy showed an unusual pattern of attack, and pre-treatment decay is suspected but not yet proved. Sounding the poles and using the Pilodyn indicated decay, but poking did not. Quant...
H Friis-Hansen


The isolation of actinomycetes from wood in ground contact and the sea
1980 - IRG/WP 1110
M S Cavalcante, R A Eaton


Bordered Pit Imaging
2012 - IRG/WP 12-10773
New findings about bordered pits will be presented using the latest microscopy techniques. Three-dimensional imagery at the nanolevel is used, and short 3-D movies will shown as part of the discussion on this topic to reveal new features that have not previously been reported in pits. The implications for both microorganism penetration through lignified cells as well as preservative penetration i...
D Mascheck, B Goodell, H Militz, M Lessard, J Jellison


Comparative studies on the distribution of lignin and CCA elements in birch using electron microscopic X-ray microanalysis
1987 - IRG/WP 1328
The microdistribution of metal preservatives in treated wood has received considerable research over the last two decades. Despite this, little effort seems to have been made to try and correlate the distribution of CCA elements with respect to naturally occurring wood cell wall components in wood. In the present preliminary study an attempt is made to relate the distribution of lignin in-situ wit...
G F Daniel, T Nilsson


Microscopic characteristics of Pinus radiata wood veneers weathered outdoors for one yea
1993 - IRG/WP 93-10018
Radiata pine (Pinus radiata) wood veneers glued to solid wood hacking strips were exposed outdoors at 45° north-facing for one year and then examined by light microscopy (LM) and transmission electron microscopy (TEM). As expected, the exposed wood surfaces were colonised by a variety of microorganisms; however, wood decay was not observed. Ultlaviolet (UV light) radiation and water were the main...
A P Singh, R A B Sweeney, E A Dunningham, D V Plackett


Effect of methylene bisthiocyanate on morphology and ultra-structure of a sapstain fungus, Ophiostoma floccosum
2003 - IRG/WP 03-10471
In vitro effects of methylene bisthiocyanate (MBT) on hyphal morphology and ultrastructure of Ophiostoma floccosum were examined using differential interference contrast, epifluorescence and transmission electron microscopy (TEM). Differential interference contrast microscopy suggested that MBT caused rapid changes to the morphology of O. floccosum resulting in excessive formation of vacuoles and...
T Singh, B Kreber, R N Wakeling, A Stewart


The Pilodyn instrument as a non-destructive tester of the shock resistance of wood
1978 - IRG/WP 2107
A new non-destructive shock resistance tester, the PILODYN, has been developed. The instrument measures the fracture surface area created by a constant amount of energy. It operates by shooting a blunt pin into wood by an exact amount of energy. The penetration depth is read on a scale. A wide field of application is open to a non-destructive shock resistance tester such as: 1) assessment of the r...
P Hoffmeyer


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