IRG Documents Database and Compendium


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Analyzing hyphal growth of the bio incising fungus Physisporinus vitreus with light-, confocal laser scanning- and, Synchrotron X-ray tomographic microscopy
2010 - IRG/WP 10-20438
Norway spruce wood is an important building material for the forest and wood industry. To improve wood permeability and uptake of wood modification substances Physisporinus vitreus is employed as a bioincising agent. In this study wood specimens were incubated with P. vitreus to assess hyphal colonisation and structural alterations of Norway spruce wood. After different incubation periods, semi-th...
C Stührk, M Fuhr, M Schubert, F W M R Schwarze


Fungal degradation of wood treated with metal-based preservatives. Part 2: Redox states of chromium
1996 - IRG/WP 96-10164
Concerns have arisen about the leaching of heavy metals from wood treated with metal-based preservatives, such as chromated copper arsenate (CCA). Of particular concern is the toxic redox state of chromium and arsenic in aging and decayed CCA-treated wood. Generally, hexavalent chromium is more toxic than trivalent chromium and trivalent arsenic is more toxic than pentavalent arsenic. The desired ...
B Illman, S Bajt, T L Highley


Water sprinkled pine wood: A microscope study on boards showing streaking
1993 - IRG/WP 93-10033
Boards sawn from the outer sapwood of pine lumber previously water sprinkled for periods of 10-18 weeks and kiln dried, showed streaking after staining. Two types of enhanced stain uptake were noted: 1) a more concentrated and localized form after 10 weeks and 2) a more diffuse type which developed in later stages of water sprinkling. Areas showing enhanced stain uptake were examined using light a...
G F Daniel, T Elowson, T Nilsson, A P Singh, K Liukko


Studies of the ray parenchyma cell ends on the radial flow of Sitka spruce (Picea sitchensis)
2000 - IRG/WP 00-40164
It is widely accepted that either ray tracheids or ray parenchyma cells offer the major flow pathways radially for the impregnation of softwood with preservative chemicals. It is now generally recognised that, if radial flow does occur through ray parenchyma cells, the cross-field pits play a dominating role for the liquid movement in the radial direction. In this case, the radial flow of fluid is...
I Usta


Imaging fungal deterioration of wood using x-ray microtomography
1997 - IRG/WP 97-10240
Nondestructive tomographic images of solid Southern yellow pine (SYP) wood and SYP during deterioration by wood decay fungi were obtained by using a new class of synchrotron X-ray computed microtomography (CMT). The CMT images will provide information about spatial relationship between degrading fungi and wood structures. Wood samples were scanned with synchrotron-generated X-rays at high resoluti...
B Illman, B A Dowd


Micro-Distribution of Metals in Wood Treated with a Nano-Copper Wood Preservative
2007 - IRG/WP 07-40360
The microdistribution of copper in southern pine treated with a newly-developed nano-copper wood preservative was examined to determine if it differed from that reported for wood treated with conventional copper-based wood preservatives. Field Emission Scanning Electron Microscopy (FE-SEM) in combination with x-ray microanalysis (EDX) revealed the presence of nano-sized copper and iron particles i...
H Matsunaga, M Kiguchi, P Evans


Copper nanoparticles in southern pine wood treated with a micronised preservative: Can nanoparticles penetrate the cell walls of tracheids and ray parenchyma?
2010 - IRG/WP 10-30547
This study tests the hypothesis that copper nanoparticles can penetrate the cell walls of southern pine wood treated with a micronised preservative. We examined the nanodistribution of particles in tracheid and ray parenchyma cell walls using state-of-the-art HR (High Resolution)-TEM and HR-STEM (Scanning Transmission Electron Microscope)-EDX. These devices are capable of atomic-scale resolution. ...
H Matsunaga, Y Kataoka, M Kiguchi, P Evans


Copper Nanoparticles in Southern Pine Wood Treated with a Micronised Preservative: Nanodistribution of Copper in the Pit Membrane and Border of an Earlywood Bordered Pit
2011 - IRG/WP 11-30566
Copper nanoparticles can penetrate the cell walls of unlignified parenchyma cells in southern pine wood treated with a micronised wood preservative, but they are excluded from lignified tracheid walls. This paper extends these observations to include the cell wall layers of the bordered pit. Focused ion beam and ion milling were used to make an ultra-thin section of the cell wall layers of an earl...
H Matsunaga, Y Kataoka, M Kiguchi, P Evans


Further Studies on the Distribution of Copper in Treated Wood Using an XRF Microscope Technique
2011 - IRG/WP 11-40549
There are two distinct forms of copper in aqueous copper based wood preservative systems that are currently used in the United States for treatment of southern pine lumber. These are systems using either soluble copper (typically amine based) or the more recent particulate or “micronized” copper as the primary biocide. Studies reported last year showed that there were distinct differences in...
A Zahora


Accessibility of Wood Cell Walls to Well-defined Platinum Nanoparticles
2012 - IRG/WP 12-20494
Copper nanoparticles are found in the walls of parenchyma cells in southern pine sapwood treated with a micronised wood preservative, but they are absent from tracheid walls. Hence, we hypothesized that small nanoparticles can penetrate the walls of unlignified parenchyma cells, but are excluded from lignified tracheid walls. This paper tests this hypothesis by treating pine sapwood with an aqueou...
H Matsunaga, Y Kataoka, M Kiguchi, P D Evans


Scanning Transmission X-ray Microscopy as a tool for examining chemical changes in wood cell walls due to enzymatic activity
2014 - IRG/WP 14-20556
As development of techniques for examining enzymatic activity on solid, complex biological materials, is considered to be an important factor in further development of enzyme applications and for understanding the decay of plant materials, this study explores applicability of Scanning Transmission X-ray Microscope (STXM) for determining chemical changes in enzymatically treated samples at the cell...
D Jeremic, R Goacher, R Yan, C Karunakaran, E R Master


Confocal laser scanning microscopy of a novel decay in preservative treated radiata pine in wet acidic soils
1997 - IRG/WP 97-10215
Light microscopy of radiata pine (Pinus radiata D. Don) field test stakes (20x20x500mm3) exposed in wet acidic (pH 3-4) soil for 12 - 24 months showed predominance of an unusual type of decay characte-rised by tunnelling attack of wood cell walls. After two years decay was moderate to severe in wood treated to ground contact CCA specifications and also equivalent retentions of creosote, and a numb...
R N Wakeling, Ying Xiao, A P Singh


Monographic information on Schizophyllum commune
1975 - IRG/WP 118 E
D Dirol, M Fougerousse


Resistance of Alstonia scholaris vestures to degradation by tunnelling bacteria
1992 - IRG/WP 92-1547
Electron microscopic examination of vessels and fibre-tracheids in the wood of Alstonia scholaris exposed to tunnelling bacteria (TB) in a liquid culture showed degradation of all areas of the secondary wall. The highly lignified middle lamella was also degraded in advanced stages of TB attack. However, vestured pit membranes and vestures appeared to be resistant to degradation by TB even when oth...
A P Singh, T Nilsson, G F Daniel


The dry rot fungus and other fungi in houses. Part 2
1993 - IRG/WP 93-10001
J Bech-Andersen


Comparative studies on the distribution of lignin and CCA elements in birch using electron microscopic X-ray microanalysis
1987 - IRG/WP 1328
The microdistribution of metal preservatives in treated wood has received considerable research over the last two decades. Despite this, little effort seems to have been made to try and correlate the distribution of CCA elements with respect to naturally occurring wood cell wall components in wood. In the present preliminary study an attempt is made to relate the distribution of lignin in-situ wit...
G F Daniel, T Nilsson


Detection of defects in standing poles by X-ray techniques
1980 - IRG/WP 2132
The application of X-ray techniques to wood pole inspection procedures has been evaluated. Internal defects were accurately identified using X-ray inspection methods. These methods and their possible influence on the safety and economic aspects of wood pale utilization are discussed....
W D Gardner, R S Johnstone, W Pitt


A short note on fungal decay in K33-treated poles
1982 - IRG/WP 1169
Soft rot cavities and erosion of the lumen have been found in K33-treated Pinus sylvestris poles from the years 1956-66 by microscopic studies. Poor treatment quality has been proved for some of these poles. The microscopy showed an unusual pattern of attack, and pre-treatment decay is suspected but not yet proved. Sounding the poles and using the Pilodyn indicated decay, but poking did not. Quant...
H Friis-Hansen


The isolation of actinomycetes from wood in ground contact and the sea
1980 - IRG/WP 1110
M S Cavalcante, R A Eaton


Ultrastructural and TEM-EDAX studies on the degradation of CCA treated radiata pine by tunnelling bacteria
1985 - IRG/WP 1260
An ultrastructural study was carried out on bacterial attacked Pinus radiata stakes treated with a high level (24.7 kg/m³) of Tanalith NCA preservative. The purpose of the investigation was to determine whether the organism possessed intracellular and/or extracellular detoxification mechanisms in order to overcome the high levels of copper, chrome and arsenic present within the wood fibre walls. ...
G F Daniel, T Nilsson


Microscopic characteristics of Pinus radiata wood veneers weathered outdoors for one yea
1993 - IRG/WP 93-10018
Radiata pine (Pinus radiata) wood veneers glued to solid wood hacking strips were exposed outdoors at 45° north-facing for one year and then examined by light microscopy (LM) and transmission electron microscopy (TEM). As expected, the exposed wood surfaces were colonised by a variety of microorganisms; however, wood decay was not observed. Ultlaviolet (UV light) radiation and water were the main...
A P Singh, R A B Sweeney, E A Dunningham, D V Plackett


Effect of methylene bisthiocyanate on morphology and ultra-structure of a sapstain fungus, Ophiostoma floccosum
2003 - IRG/WP 03-10471
In vitro effects of methylene bisthiocyanate (MBT) on hyphal morphology and ultrastructure of Ophiostoma floccosum were examined using differential interference contrast, epifluorescence and transmission electron microscopy (TEM). Differential interference contrast microscopy suggested that MBT caused rapid changes to the morphology of O. floccosum resulting in excessive formation of vacuoles and...
T Singh, B Kreber, R N Wakeling, A Stewart


X-ray analysis of selected anatomical structures in copper/chrome/arsenic treated wood
1973 - IRG/WP 320
Application of analytical electron microscopy to problems in wood preservation has been very limited. Indeed, less than ten workers appear to have published their results using the technique, and of these' only two papers deal with energy dispersion procedures in the scanning electron microscope; the others employ the more familiar wavelength dispersive methods of the electron probe....
H Greaves


A wood preservative for the future: Copper dimethyldithiocarbamate
1994 - IRG/WP 94-30045
The development of a new wood preservative, copper dimethyl-dithiocarbamate (CDDC) is reviewed in this paper. CDDC is formed in situ by dual pressure treatments. Laboratory and field efficacy trials, physical and chemical properties of the preservative solutions and treated wood, and plant handling characteristics of the system are examined....
D K Stokes, M H Freeman, T L Woods, R D Arsenault


Monographie de Schizophyllum commune
1975 - IRG/WP 118
D Dirol, M Fougerousse


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