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Natural durability of larch heartwood against decay
1997 - IRG/WP 97-10201
Larch heartwood has been promoted in Sweden and in other European countries as an alternative to preservative treated timber. It has even beeen stated that larch heartwood is more durable than preservative treated timber. This paper presents a compilation of facts from scientific reports on the durability of larch heartwood. Some recent data from this department are also reported. All data suggest...
T Nilsson


Solid state NMR study on pine wood degraded by brown rot fungus Gloeophyllum trabeum
1994 - IRG/WP 94-10067
Blocks of Pinus koraiensis wood were degraded by Gloeophyllum trabeum and examined by solid-state 13C NMR spectroscopy. Chemical compositions were estimated from relative signal aras. There was no evidence for preferential degradation of the noncrystalline cellulose which accounted for about 4% by weight of the wood. Loss of partly ordered cellulose on crystal surfaces exceeded the loss of crystal...
Yoon Soo Kim, R Newman


New ways of chemical wood preservation
1997 - IRG/WP 97-40087
Synergistic effects pertaining to wood preservation have been investigated by combining different polymerizing agents with modifying chemicals, modifying chemicals with known preservatives and new types of preservative chemicals with each other. One of the investigated methods is the use of impregnating solutions based on furfuryl alcohol (produced from biomass), in which the chemicals polymerizes...
M Westin, T Nilsson, B Ohlsson, R Simonson


The use of selective media for studying the colonization of wood in ground contact by microorganisms
1978 - IRG/WP 186
In October1976 a field trial was set up in order to monitor the progressive colonisation of wood in ground contact using orientated small stakes of Scots pine (Pinus sylvestris) and birch (Betula sp.) (30 x 45 x 250 mm³). Half the number of each species were treated with a 1% solution of CCA by a vacuum impregnation process. All the stakes were coated on three sides with an epoxy-resin sealant to...
C P Clubbe


Redox regulation of enzyme activity during wood decay
1996 - IRG/WP 96-10172
A potential strategy in the search for alternative wood preservatives against fungal decay is to target the extracellular wood-decay process itself, rather than the decay organisms. This presents novel targets for selective disruption and possibly without the broad-spectrum toxicity associated with conventional wood preservatives. The enzymes of white rot decay are mechanistically diverse (e.g. hy...
P J Kersten, B Kurek, J W Whittaker


Application of non-destructive techniques (durometric and ultrasonic) to evaluate the degradation of woods in service by Gloeophyllum trabeum
2001 - IRG/WP 01-20220
In order to establish the degree of degradation of wood used in construction, it is necessary to employ non-destructive methods (ultrasonic, durometric, densitometric). In this study, ultrasonic and Pilodyn durometric techniques have been applied to try to establish parameters of relationship between the values obtained by both, for their immediate application to wood in service. As a method of re...
M T De Troya, L Palaia, A Navarrete, V Galvañ, R Molina, A Guijarro, J Camacho


Detection of dry rot by air analysis
1992 - IRG/WP 92-2399
Detection of dry rot (Serpula lacrymans) at an early stage appears to be very difficult in practice. Also control inspections in buildings, after remedial treatment of dry rot, have a limited accuracy. The use of trained dogs in Denmark initiated the idea for this research on the possible use of air analysis as a detection method. The Centre for Timber Research-TNO (TNO-CHT) and the Institute for ...
P Esser, A C Tas


The colonisation and succession of fungi in wood
1980 - IRG/WP 1107
A study of the microbial ecology of small stakes of Pinus sylvestris and Betula pendula, both untreated, and treated with a 1% solution of CCA is in progress. Results are presented for the early stages, up to 18 months.Two new techniques were developed linked by the philosophy of objective assessment. Isolations were made from the wood in a pre-determined pattern and transferred to four selective ...
C P Clubbe


rDNA-ITS sequence of Serpula lacrymans and other important indoor rot fungi and taxon-specific priming PCR for their detection
1999 - IRG/WP 99-10298
Taxon-specific priming polymerase chain reaction (TSPP) is a powerful molecular tool for fungal diagnosis. For its application to indoor rot fungi, the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) of the main fungal species causing wood rot in European buildings was amplified with the polymerase chain reaction (PCR). The ITS region was sequenced. The complete sequences are present...
O Schmidt, U Moreth


Silicic acid-Boric acid complexes as wood preservatives
2001 - IRG/WP 01-30273
A silicic acid monomer aqueous solution (SAMS) or colloidal silicic acid solution (CSAS) was combined with various metal compounds or boric acid. Agents where SAMS or CSAS was combined with boric acid gave good protection against decay caused by the brown-rot fungus Fomitopsis palustris, the treated wood (Cryptomera japonica D. Don) specimens after the leaching test maintained a high resistance to...
H Yamaguchi


Wood and filter paper degradation, phenol oxidase and one-electron oxidation activities by the white rot fungus Ceriporiopsis subvermispora
2003 - IRG/WP 03-10486
The activities of one-electron oxidation and phenol oxidase during incubation of cultures of the white-rot basidiomycete Ceriporiopsis subvermispora containing either glucose or wood were periodically measured. Further, the degradation activities against wood and filter paper were examined during the course of cultivation. Weight losses of Japanese beech wood and Japanese cedar wood after 12 wee...
H Tanaka, S Itakura, A Enoki


Evaluation of bending strength by non-destructive methods of Ezomatzu with white pocket rot
1991 - IRG/WP 2371
The possibility of application of three non-destructive methods was examined for evaluation of bending strength of ezomatsu (Picea jezoensis CARR.) with white pocket rot. As non-destructive methods, the measurements of ultrasonic wave propagation time, impact-induced stress wave propagation time and penetration depth of pin of "PILODYN Wood Tester" were applied to compare with visual evaluation of...
T Tanaka


Development of a mini-block test method for the rapid evaluation of preservative performance against Basidiomycte fungi
1991 - IRG/WP 2379
Screening procedures for new biocides used as wood preservatives can be a time consuming process using conventional Basidiomycete assay procedures. This is due mainly to the long exposure periods required to achieve adequate levels of decay (weight loss) in the test blocks. A number of alternative methods have been developed but most utilise artificial substrates such as filter paper or agar in wh...
J Brown, S Caswell, G R Williams


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson


Evaluating the performance of wood preservatives against fungi
1974 - IRG/WP 247
A compilation is made of results published by various workers for agar/block laboratory tests of the effectiveness of wood preservatives against 11 species of wood destroying Basidiomycetes and one species of soft rot fungus. Preservatives include creosote, boron, metal/arsenic mixtures, metallic naphthenates, chlorinated naphthalene, chlorinated phenols and tri-n-butyl tin oxide. The results are ...
R Cockcroft


The role of oxygen and oxygen radicals in one-electron oxidation reactions mediated by low-molecular weight chelators isolated from Gloeophyllum trabeum.markup
1994 - IRG/WP 94-10086
The KTBA assay for determination of one-electron oxidation activity was used to assay reactions of low-molecular weight chelators isolated from the brown rot fungus Gloeophyllum trabeum. The assay, performed either under air or nitrogen showed that molecular oxygen was an important factor in chelator-mediated oxidation reactions. A reduction in oxidative activity was observed when superoxide dismu...
Jun Lu, B Goodell, Jiang Liu, A Enoki, J Jellison, H Tanaka, F Fekete


Micromorphology of oak wood degraded by brown rot fungus Coniophora puteana
2000 - IRG/WP 00-10356
To characterize the degradation of hardwood by brown rot fungus, Coniophora puteana was incubated on the wood blocks of Quercus accutissima. For comparison, the same fungus was also incubated on the pine wood blocks of Pinus densiflora. In softwood, the shape of the wood cells and thickness of the cell appeared unchanged even the loss of birefringence in the affected areas. In contrast, oak wood d...
Yoon Soo Kim, Seung-Gon Wi, Kwang-Ho Lee


Investigations on testing with soft rot fungi by the vermiculite-burial method
1972 - IRG/WP 215
With the object of developing a test method to determine the effectiveness of wood preservatives against soft rot attack, tests have been carried out with pure fungal cultures using vermiculite as the substrate for untreated and preservative impregnated wood blocks. In these tests the suitability of common commercial, simple disposable beakers was proved successful as a substitute for the glass bo...
W Kerner-Gang, M Gersonde


Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


The effect of copper/chrome/arsenic (CCA) treated timber on soil fungi
1981 - IRG/WP 1131
The aim of this investigation was to study the effect of CCA treated wood on the microflora of soil with particular reference to copper tolerant soft rot fungi. The IRG collaborative field experiment was designed to monitor the performance of a range of preservative treated hardwoods with particular reference to soft rot in CCA treated timber (Dickinson 1976) and site 33 of this experiment was use...
R J Murphy, D J Dickinson


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


Preliminary investigation on the natural durability of Guayule (Parthenium argentatum)-based wood products
2000 - IRG/WP 00-40154
Conventional preservatives used to protect wood from insect and microbial damages are presently of major concern to human health and the environment. Finding alternative and economical preservatives has not been successful. Previous studies have shown that the resinous material extracted from the guayule plant (Parthenium argentatum, Gray) has both insect- and microbial-resistant properties. Unfor...
F S Nakayama, P Chow, D S Bajwa, J A Youngquist, J H Muehl, A M Krzysik


List of fungi in soft-rot tests
1975 - IRG/WP 105
This list gives information on the terrestrial and marine species and strains of fungi tested for soft-rot ability in pure culture with data on their decay activity in untreated wood. An index of the timber species tested is added. The compilation is based on 1) the list of soft-rot fungi of the former OECD Group (Doc.No. 27/DAS/CSI/M/559), Oct. 1968, prepared by R. Rösch and W. Liese, 2) informa...
G Seehann, W Liese, B Kess


Degradation of resin constituents in various wood species by the white rot fungus Bjerkandera sp. strain BOS55
1999 - IRG/WP 99-10301
In previous studies, the white-rot fungus Bjerkandera sp. strain BOS55 was shown to cause extensive degradation of lipophilic extractives (resin) in Scots pine wood. Further research was carried out in order to investigate the ability of Bjerkandera sp. for reducing resinous constituents in various softwood (Douglas fir, larch and spruce) and hardwood species (birch, beech and poplar). The greates...
J Dorado, T A Van Beek, F W Claassen, R Sierra-Alvarez


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