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Ability of chitosans to limit wood decay under laboratory conditions.
2004 - IRG/WP 04-30339
Chitosans have recently been explored for controlling a number of plant pathogenic fungi and have shown some promise for controlling stain fungi on wood. The potential for using chitosans either alone or in combination with copper sulfate to protect wood from fungal decay was explored using maple veneers. Chitosan treatment reduce weight losses caused by two brown rot and one white rot fungi, howe...
M Maoz, J J Morrell


Generation of hydroxyl radical by the brown-rot fungus, Postia placenta
1988 - IRG/WP 1360
In an electron spin resonance (ESR) survey of various liquid cultures and wood slivers innoculated with the brown-rot fungus, Postia placenta, the spin trap dimethyl-l-pyrroline N-oxide (DMPO) was used to detect the presence of the hydroxyl radical. The ESR spectra for the paramagnetic DMPO- hydroxyl radical adduct was observed in (1) nitrogen-limited, liquid cultures having 1.0% glucose or 0.1% c...
B Illman, D C Meinholtz, T L Highley


Accelerated laboratory testing of preservatives on 13 North American wood species
1999 - IRG/WP 99-30201
The ability of selected water and solvent based biocides to protect wood against fungal attack was evaluated on 13 North American wood species using 7 decay fungi in a modified soil block test. Most preservatives were capable of providing protection at their recommended above ground and soil contact use levels. Many decay fungi caused substantial weight losses on blocks treated with chlorothalonil...
J J Morrell, C M Freitag


Fenton's reagent as a modification tool in brown-rot decay
1996 - IRG/WP 96-10155
A biomimetic approach was used to clarify the role and importance of the Fenton-type reaction in the carbohydrate degradation by brown-rot fungi. Spruce sawdust and microcrystalline cellulose were modified in the H2O2/Fe(II) treatment. The degree of hydrolysis of the pretreated spruce sawdust was clearly increased with the complete cellulase (Econase), purified endoglucanase from Trichoderma reese...
M Rättö, A-C Ritschkoff, J Buchert, L Viikari


Preservative effect of cellulose insulation material against some mould fungi and brown rot fungus Coniophora puteana in pine sapwood
1991 - IRG/WP 1484
The influence of mineral wool and loose-fill cellulose wool on biodeterioration of pine sapwood was studied. The test fungi were mould fungi Aspergillus versicolor, Cladosporium sphaerospermum, Penicillium sp. and Aureobasidium pullullans and brown rot fungus Coniophora puteana. Cellulose wool inhibited the growth of mould fungi on the face of wood contacted with insulation material at RH of 97 an...
H Viitanen


Statistical evaluation of 'micro-bending' samples for classification of wood attacking fungi
1992 - IRG/WP 92-1544
Micro-specimens from both hardwood and softwood were exposed to selected species of field isolated fungi. The strength reduction and weight loss caused by these fungi were determined. A statistical procedure was then developed to classify the fungi into brown-, white-rot, or non-decay fungal categories based on these data. The procedure reveals the extent of hazard a fungus poses to wood members. ...
B Goodell, Jing Liu, A Homola, J Jellison, J Shottafer


Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia placenta
1989 - IRG/WP 1391
Scanning electron microscopy (SEM) of Pinus sp. decayed by the brown-rot fungus Postia placenta confirmed the existence of extracellular membranous structures previously described by transmission electron microscopy (TEM). These structures appear to be an integral part of the hyphal sheath and assume a variety of forms including lamellar sheets, fibrils, and vesicles. These structures were observe...
F Green III, M J Larsen, L L Murmanis, T L Highley


Production of exraxellular hydrogen peroxide and oxalic acid by brown-rot fungus Poria placenta
1995 - IRG/WP 95-10112
Hydrogen peroxide and oxalic acid have been suggested to be essential in the degradation of wood carbohydrates by brown-rot fungi. Hydrogen peroxide has been suggested to be one of the diffusible low molecular weight agents produced by brown-rot fungi for the degradation of wood carbohydrates by generating highly active radicals. The production of hydrogen peroxide and oxalic acid by Poria placent...
A-C Ritschkoff, M Rättö, J Buchert, L Viikari


Early detection of brown-rot decay in southern yellow pine using immunodiagnostic procedures
1990 - IRG/WP 2356
Immunodiagnostic procedures have been used to detect incipient decay of southern yellow pine by six common brown-rot fungi. Rabbit polyclonal antibodies were raised to liquid culture fractions of the six fungi. The antibodies, after preadsorption to sawdust, were tested in particle agglutination assays, immuno-dot blot, and ELISA for their·ability to detect the decay organisms when they were grow...
C A Clausen, F Green III, T L Highley


Detection of a brown-rot fungus using serological assays
1986 - IRG/WP 1305
Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also u...
B Goodell, J Jellison


Extracellular hydrogen peroxide producing and hydrogen peroxide reducing compounds of wood decay fungi
1991 - IRG/WP 1516
Extracellular H2O2-producing and H2O2-reducing compounds were isolated from wood-containing cultures of all the white-rot and brown-rot fungi and Ascomycetes which well degraded wood, but were not detected in the culture of the fungi which degraded little wood. The compounds are glycopeptides with a low molecular weight, require H2O2 for one-electron oxidation, catalyze the redox reaction between ...
A Enoki, G Fuse, H Tanaka


Decay resistance of densified ammonia-plasticized stems of oil palm (Elaeis guineensis)
1991 - IRG/WP 3673
When wafers of oil palm stems (Elaeis guineensis) were plasticized with 28% aqueous ammonia and immediately compressed mechanically, the treated material, gained 73% in basic density (average basic density, 0.695 g/cm³), and was highly resistant to decay by wood rot basidiomycetes. Compared with the control specimens (density, 0.403 g/cm³), resistance to decay of the densified specimens by Corio...
A H H Wong, M P Koh


Dimensional lumber model demonstrates the sensitivity of the particle capture immunoassay in early detection of brown-rot fungi
1995 - IRG/WP 95-20058
A dimensional lumber model was designed to test the sensitivity of the particle capture immunoassay (PCI) compared to traditional methods of culturing and microscopic examination for detection of brown-rot fungi. Southern yellow pine 2 x 4's (5.1x10.2 cm²), and 86.4 cm in length, were inoculated on one end with the brown-rot fungus, Postia placenta, and tested over the full length of the...
C A Clausen, L Ferge


Hemicellulosic induction of oxalic acid in Postia placenta
1994 - IRG/WP 94-10060
Most studies suggest that enzymes produced by brown-rot fungi are too large to penetrate sound wood structures, even after decay begins. Thus, nonenzymatic agents have been proposed to initiate brown-rot decay. We have reported that the brown-rot fungus Postia placenta MAD-698 initiates a 2-fold decrease in wood pH within 7 days of colonization which is mediated by production of oxalic acid. Strai...
F Green III, M Larsen, T L Highley


Premature failure of CCA treated vineyard posts from brown rot
1993 - IRG/WP 93-10016
A survey of CCA (AWPA Type C) treated posts used as grape vine trellis supports, to determine the extent and type of decay was carried out at a terraced vineyard in the Hawkes Bay region of the North Island of New Zealand. Of 357 posts examined out of a total of 970 in the vineyard, 47% had moderate to severe decay or had failed, 20% had established decay, 10% had a trace of decay and 23% had no s...
R N Wakeling, A P Singh


Relationship between degradation of wood, cellulose or lignin-related compounds and production of hydroxyl radical or accumulation of oxalic acid in cultures of brown-rot fungi
1994 - IRG/WP 94-10062
The degradation activities of brown rot fungi against wood, cellulose, and lignin-related compounds were measured in cultures containing glucose or wood as a carbon source. Also the activities of one-electron oxidation and hydroxyl radical production and the amount of oxalic acid present in the cultures were measured. The degradation activities of the fungi against wood, crystalline cellulose and ...
S Itakura, T Hirano, H Tanaka, A Enoki


Lab and field test results for wood treated with polymeric alkylphenol polysulfide
2001 - IRG/WP 01-30268
A new wood preservative based on polymeric alkyphenol polysulfide (PXTS-A) is being evaluated in a series of laboratory and field tests and the results continue to show promise. A modified formulation (PXTS-B), which contains additives to reduce the viscosity, is being evaluated along with the original formulation. Soil block decay tests comparing PXTS-B with CCA-C indicate that PXTS-B is equally ...
J Goswami, A Abramson, R Buff, D D Nicholas, T Schultz


Relative merits of laboratory and field tests for assessing wood decay resistance
1993 - IRG/WP 93-20009
Laboratory and field trials have long been employed for assessing natural durability of a variety of wood species, but there is a continuing debate concerning the relative merits of approach. While laboratory tests provide carefully controlled conditions for decay, they are often criticized for being too artificial to produce meaningful results. Conversely, field trials often vary widely between t...
T C Scheffer, J J Morrell


Micromorphology of decay in Keruing heartwood by the basidiomycetes Phellinus contiguus and Dacrymyces stillatus
2002 - IRG/WP 02-10454
This study aimed to characterise the micromorphology of fungal decay in a wooden handrailing in an above ground exposure at the Thames embankment in London. Keruing heartwood (Dipterocarpus spp.) was determined as the construction timber and the two basidiomycetes Phellinus contiguus and Dacrymyces stillatus identified as main decay organisms. Their decay patterns within Keruing heartwood were...
G Kleist, M Ray, R J Murphy


The nature of osmiophilic particles and their distribution during different stages of brown and white rot decay
1983 - IRG/WP 1213
The distribution of osmiophilic particles during the course of brown and white rot decay was investigated by applying transmission electron microscopic (TEM) methods. It was found that it correlates with the brown and white rot pattern.The osmiophilic particles are produced by the fungus and are supposed to be wood rotting enzymes....
K Messner, H Stachelberger


Targeted inhibition of wood decay fungi: degradation of cotton cellulose
1999 - IRG/WP 99-10321
Brown-rot decay is responsible for 80% of the damage and replacement of wood in service. For nearly thirty years, researchers have postulated a one-electron oxidase system combining Fe+2 oxalic acid, and H2O2 in the production of hydroxy radicals which ultimately oxidize cellulose in situ. Recently, researchers have been investigating antioxicants, in combination with biocides (Schultz, et al.,199...
F Green III, T A Kuster


Decay resistance of Siberian larch wood against brown rot fungus. - Part 3. The variation between plus trees and their grafted clones
2000 - IRG/WP 00-10350
The aim of the study was to estimate the variation in the decay resistance of Siberian larch (Larix sibirica Ledeb.) wood using mature plus trees and their grafted clones. Samples were collected with an increment core borer from 11 original plus trees and their 25-year-old grafted seed orchard clones. A typical brown rot fungus, Coniophora puteana (Schum. ex Fr.) Karst. (BAM Ebw. 15), was used as ...
H Viitanen, L Paajanen, M Venäläinen, A Harju, P Velling


Natural durability of larch heartwood against decay
1997 - IRG/WP 97-10201
Larch heartwood has been promoted in Sweden and in other European countries as an alternative to preservative treated timber. It has even beeen stated that larch heartwood is more durable than preservative treated timber. This paper presents a compilation of facts from scientific reports on the durability of larch heartwood. Some recent data from this department are also reported. All data suggest...
T Nilsson


New ways of chemical wood preservation
1997 - IRG/WP 97-40087
Synergistic effects pertaining to wood preservation have been investigated by combining different polymerizing agents with modifying chemicals, modifying chemicals with known preservatives and new types of preservative chemicals with each other. One of the investigated methods is the use of impregnating solutions based on furfuryl alcohol (produced from biomass), in which the chemicals polymerizes...
M Westin, T Nilsson, B Ohlsson, R Simonson


A possible role of unique TCA cycles in wood-rotting basidiomycetes
2003 - IRG/WP 03-10461
The copper tolerant brown-rot fungus, Fomitopsis palustris, acquires metabolic energy by use of the constitutively-occurring Kornberg’s glyoxylate cycle coordinating with oxalate biosynthesis and glucose oxidation (Erman Munir et al. Proc. Natl. Acad. Sci. USA, (2001) 98, 11126-11130). Furthermore, this fungus does not have the normal TCA cycle, lacking 2-oxoglutarate dehydrogenase which is a ke...
E Munir, T Hattori, M Shimada


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