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Gloeophyllum trabeum and Gloeophyllum abietinum, the most frequent brown rot fungi in fir wood joinery
1999 - IRG/WP 99-10319
In Croatia the primary raw material for joinery production is silver fir wood (Abies alba Mill). L-joints made of home-grown fir sapwood and prepared according to EN 330: 1993 were used to establish the infection and colonisation of micro-organisms, particularly wood decay fungi, to compare the performance of untreated and 1% TnBTO treated L-joints. The L-joints were coated with two types of coat,...
R Despot, M Glavas


The influence of crystalline and amorphous cellulose on extracellular hydrogen peroxide production by brown-rot fungi
1991 - IRG/WP 1482
The production of hydrogen peroxide (H2O2) has been suggested to play a key role in the degradation of wood by wood-rotting fungi. The production of extracellular hydrogen peroxide was studied by a quantitative method which detects the oxidation of the 2,2-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) by H2O2 and horseradish peroxidase (HRP) in liquid culture medium. The carbon sources u...
A-C Ritschkoff, L Viikari


Resistance to soft rot of hardwood plywood treated with CCA salt
1983 - IRG/WP 3258
Plywood made from indigenous hardwoods was treated at an average loading of 34 kg Celcure A per m³ and was installed in a field test. After 20 years the samples were only slightly attacked by soft rot and the glue bonds were still intact....
R S Johnstone


Efficacy of anhydrides as wood protection chemicals - II. Performance against soft rot fungi
1998 - IRG/WP 98-30174
Pine sapwood modified with various anhydrides and with butyl isocyanate was tested for its resistance to soft rot decay. Small stakes were exposed for 20 months in unsterile soil in a fungal cellar test. Wood modified with butyl isocyanate performed better than any of the anhydrides tested, with a threshold level of protection (less than 3% weight loss) at 12% weight percent gain (WPG). Stakes ac...
S C Forster, M D C Hale, G R Williams


Electron microscopic detection and chemical analysis of three-lamellar structures in wood-destroying fungi
1984 - IRG/WP 1240
In the course of transmission electron microscopical investigations of pine wood decay by various brown- and white-rot fungi extracellular three-lamellar structures (TLS) formed by the fungi were found in specimens stained with ruthenium red. These structures occured in the lumen of the wood cell surrounding the hypha at the outermost layer of the fungal cell wall. In the course of the investigati...
R Foisner, K Messner, H Stachelberger, M Röhr


Cytochemical localization of hydrogen peroxide in brown rot fungus Tyromyces palustris by cerium chloride technique
1999 - IRG/WP 99-10299
Cerium chloride (CeCl3) was used to localize H2O2 cytochemically for studying relationship between ultrastructural and functional characteristics of cellulose degradation by brown rot fungi. This technique proved very useful in localizing discrete electron-densereactionproducts at high resolution with minimal nonspecific deposition. The cytochemical localization of extracellular H2O2 by CeCl3 usin...
Yoon Soo Kim, Seung-Gon Wi


Some physiological characteristics of a non-degradative strain of Postia (=Poria) placenta
1988 - IRG/WP 1341
The decay capacity of 14 strains of the brown-rot fungus Postia (= Poria) placenta was determined using soil-wood block tests. One isolate was identified as being unable to degrade wood and was determined to be monokaryotic. It retained the ability to produce extracellular carbohydrate-degrading enzymes, although regulation of certain enzymes was atypical under certain cultural conditions. It prod...
J A Micales, T L Highley


Extracellular layers of wood decay fungi and copper tolerance
1983 - IRG/WP 1180
Extracellular layers around the hyphae of brown, white and soft rot fungi have been examined using electron microscopy. These layers were isolated for identification. Particular interest was directed towards the extracellular layers of copper-tolerant soft rot fungi....
D M Francis, L E Leightley


Decay patterns observed in butylene oxide modified ponderosa pine attacked by Fomitopsis pinicola
1983 - IRG/WP 1183
Small blocks of ponderosa pine chemically modified by butylene oxide to three different weight percent gains (WPG) were decayed for 2 months with the brown rot fungus Fomitopsis pinicola. Wood substance loss and the type of decay pattern recognised were fairly similar both for control and blocks treated to 8 and 15 WPG. No difference in attack was observed between radial or tangential walls in lat...
T Nilsson, R M Rowell


Short term preconditioning of preservative-treated wood in soil contact in relation to performance in field trials
2000 - IRG/WP 00-20185
The effect of pre-exposure to primary colonising micro-organisms on preservative-treated wood, prior to a basidiomycete decay test, was determined by preconditioning in two soil types. Scots pine EN 113 blocks treated with 3 model systems (a triazole, a copper quaternary compound and a copper boron triazole) were leached according to EN 84 and subjected to 6 weeks and 8 weeks burial in either John...
S Molnar, D J Dickinson


Detection of brown rot decay in wood by ion mobility spectrometry
1991 - IRG/WP 2377
A test method for the detection of brown rot decay in wood is described and involves a two-step procedure: thermal release of vapours from wood samples followed by analysis using Ion Mobility Spectrometry (IMS), with results being obtained in 5-10 seconds. The advantages, limitations as well as the detection limit of the technique are presented....
A H Lawrence, T Nilsson


Elimination of alternative explanations for the effect of iron on treated wood
1993 - IRG/WP 93-30006
Amounts of iron which had previously been found in stakes removed from ground contact reduced decay of untreated wood by four brown-rot fungi. This suggested that the effect of iron may be on the preservative. Analysis of the leachates from CCA- and ACA-treated wood blocks first exposed to rusting iron, then to a brown-rot fungus, showed that the increased decay found in the laboratory for wood ex...
P I Morris, J K Ingram, D L Gent


Prevention of brown-rot decay by chelators
1992 - IRG/WP 92-1540
In this work the brown-rot decay was shown to be prevented by chelating the endogenous metals existing in wood by using organic or inorganic chelators or iron-binding siderophores. The fungal growth and decaying ability were significantly decreased by the chelating treatments of the solid wood-based culture medium and pine wood pieces, respectively. The transition metals existing in native wood ar...
L Viikari, A-C Ritschkoff


An insight into brown rot decay of timber as revealed by 13C CP/MAS NMR
1985 - IRG/WP 1259
L E Leightley


Localization of oxalate decarboxylase in the brown-rot fungus Postia placenta
1996 - IRG/WP 96-10161
Oxalate decarboxylase, the enzyme that breaks oxalic acid down into formic acid and carbon dioxide, was recently detected in mycelial extracts of the brown-rot fungus Postia placenta. Differential centrifugation was used to demonstrate that the enzyme is loosely associated with the hyphal surface. Enzyme activity can be removed by washing the hyphae with a low pH buffer. Only low levels of activit...
J A Micales


Non-enzymatic Gloeophyllum trabeum decay mechanisms: Further study
2001 - IRG/WP 01-10395
Information will be presented on the mechanisms involved in, and potential application of, non-enzymatic wood decay by brown rot decay fungi. Specifically, the hypothesized role of low molecular weight phenolate derivatives will be discussed in relation to non-enzymatic degradation of wood. The mechanism of binding of iron by cellulose, and binding and reduction of iron by fungal derivatives and m...
B Goodell, J Jellison


The potential of 2-deoxy-D-glucose as an active ingredient in wood preservation
1999 - IRG/WP 99-30205
2-deoxy-D-glucose (2-DOG) is a potential active ingredient against wood decaying fungi. When dissolved in water, it can be used in pressure treatment of wood. Thereby the wood is protected from attack by wood decaying fungi. A concentration of 1.5% (mean retention 9.7 kg/cubic meter sap-wood) is adequate for brown rot fungi, and 3% (mean retention 19.5 kg/cubic meter sapwood) will also provide protection against white rot fungi. 2-DOG is easily soluble in water, and is therefore easily leached from the wood upon completion of the preservation process. Different types of fixa-tion methods have been tried and evaluated. It is possible to produce 2-DOG by the hydrolysis of chitin, a constituent of the exterior skeleton of shellfish and insects. There is a potential for exploita-tion of this waste product provided by the crab and shrimp industry. The yield of 1 kg of fresh shrimp is 75 g of 2-DOG.
O V Frederiksen, A P Koch


Conditions for basidiospore production in the brown rot fungus Gloeophyllum separium in axenic culture
1984 - IRG/WP 1232
Attempts to control and optimize the production of hymenial structures and basidiospore production in Gloeophyllum sepiarium in axenic culture resulted in the proposal of the following conditions as being suitable. The dikaryotic mycelia originally isolated from basidiocarps could consistently be induced to produce hymenial structures and pure basidiospore collects if illuminated by near ultraviol...
J Bjurman


Detection of incipient brown rot decay in wood by fourier transform infrared spectrometry
1987 - IRG/WP 2275
Fourier transform infrared spectrometry was evaluated as a possible method for detecting different levels of brown-rot decay in pine sapwood. By using first derivative spectra and regression analysis, an equation based on 4 absorption peaks, normalized with an internal standard peak, was developed. The correlation between weight loss and absorption was highly significant indicating that this is a ...
D D Nicholas, T Schultz


Evaluation of the leach resistance and preservative efficacy of novel biocides as surface treatments applied by brush to spruce (Picea abies)
1998 - IRG/WP 98-30170
The comparative leach resistance and preservative efficacy of a number of alternative organic preservatives (available from Zeneca Specialties) and selected commercially available biocides as surface coatings to wood in Hazard Class 3 have been assessed. Two concentrations of test preservatives were applied by brush to Spruce (Picea abies), including formulation and untreated controls. A leaching ...
I M Tierney, A Bruce, D C R Sinclair, T Yeates


Effects of some essential oils on wood destroying organisms
1993 - IRG/WP 93-10047
Three wood destroying fungi: Botryodiplodia theobromae Pat. (stain), Gloeophyllum sepiarium (brown rot), and Pycnoporus sanguineus (white rot) were exposed to six plant essential oils: the peppermint, kaffir lime or leech lime, lavender, tarragon, holy basil, and the eucalyptus. The peppermint oil showed most effective to inhibit fungal growth, while eucalypus oil was the least effective. The othe...
K Atisongkroh, C Anantachoke, P Lekuthai, S Pensook, T Kittirattrakarn


A summary of tests and practical experiences with the Pilodyn wood testing instrument
1980 - IRG/WP 282
This paper presents a summary of the reports, tests and practical experiences with the Pilodyn wood tester not only, however, concerning poles but also in other fields such as standing trees, sawn timber etc. The principle of the Pilodyn is a spring-loaded pin which is fired into the object and the depth to which the pin penetrates is correlated to physical and mechanical properties of the object....
H Friis-Hansen


Final draft of a monographic card for Pole Fungus A, L Harmsen, Rigidoporus vitreus (Fr.) Donk according to the 'Model Questionnaire for preparation of monographic cards for wood-destroying fungi'
1978 - IRG/WP 178
L Harmsen


Basidiosporogenesis by brown-rot basidiomycetes in vitro
1995 - IRG/WP 95-10126
Basidiospores of wood-rotting basidiomycetes are a primary sourse of infection causing wood biodeterioration, especially in wood above ground. Most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the brown-rot fungi Antrodia car...
S C Croan


Immunogold labelling of size marker proteins in brown rot-degraded pine wood
1990 - IRG/WP 1428
Pine wood degraded by Fomitopsis pinicola was infiltrated with a mixture of ovalbumin (45 kDa) and myoglobin (16.7 kDa). After crosslinking of the proteins with glutaraldehyde and preparation for electron microscopy ultrathin sections were labeled with gold-conjugated antibodies. Simultaneous labeling of both proteins on the same section showed that at 50-70% weight loss ovalbumin did not penetrat...
E Srebotnik, K Messner


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