IRG Documents Database and Compendium


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The use of C CP/MAS NMR in the chemical identification of decayed and undecayed, tropical timber species
1984 - IRG/WP 1224
13C CP/MAS NMR was found to be an extremely powerful tool for elucidating the chemical composition of Eucalyptus maculata, Pinus elliottii and Alstonia scholaris. The differences in lignin composition were different for each timber and discussed in relation to decay caused by soft-rot and white rot fungi. In particular the presence of syringyl and guaiacyl lignin types are discussed.
L E Leightley


The dry rot fungus Serpula lacrymans, its growth and damaging of wood
2004 - IRG/WP 04-10511
The dry rot fungus (Serpula lacrymans) is one of the most dangerous wood rotting fungi, especially in the built environment. In our mycological laboratory more experiments with this fungus have been carried out aimed at:- its growth under stable and variable climatic conditions, - its possibility to attack either natural wood of different species and also modified wood primary pre-treated with ...
L Reinprecht


Identification of terminal structures in cellulose degraded by the brown-rot fungus Postia placenta
1989 - IRG/WP 1389
To gain insight into the biochemical mechanism employed by brown-rot fungi to depolymerize cellulose, we identified the end-groups of chemically pure cellulose that had been depolymerized by the brown-rot fungus, Postia placenta. The depolymerized cellulose was acid hydrolyzed and the anion fractions isolated by ion chromatography. Sugar acids were identified by gas chromatographic and mass spectr...
T K Kirk, T L Highley, R E Ibach, M D Mozuch


Occurrence, prevention and repair of Dry Rot
1990 - IRG/WP 1439
Information about the frequency, detection, identification and repair of Dry Rot attacks (Serpula lacrymans) is summarized from 13 European countries. Based on 28 completed and returned questionnaires it appears that Dry Rot attacks are recognized in all countries participating, and that there is an appreciable similarity in the frequency of attacks and methods of detection and identification. How...
A P Koch


Molecular studies on house rot fungi by RAPD-PCR
1997 - IRG/WP 97-10195
For genetic information and in view as a possible diagnosis method various isolates of the dry rot fungus, Serpula lacrymans, the wild merulius, S. himantioides, and the cellar fungus, Coniophora puteana, from Asia, Europe and USA were investigated by the polymerase chain reaction (PCR) based technique of randomly amplified polymorphic DNA (RAPD). The banding patterns obtained revealed for S. lacr...
O Schmidt, U Moreth


Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing
2004 - IRG/WP 04-10512
Fungi selected in test fields in Germany and Estonia and fungi (Coniophora puteana) cultivated on medium have been identified by the molecular methods PCR (Polymerase Chain Reaction), T-RFLP (Terminal Restricted Fragment Length Polymorphism) and sequenced to species level. The samples from the German field test showed that the fruit body sample was a different fungus than from the mycelium sample....
U Råberg, N Högberg, C J Land


Old and new facts on the dry rot fungus Serpula lacrymans
1991 - IRG/WP 1470
The article collates some of the recent literature on the biology of the dry rot fungus Serpula lacrymans. The fungus can grow at 28°C, and maximum wood moisture is above 55%. Serpula Iacrymans degrades crystalline cellulose. The intensive production of extracellular oxalic acid is neutralized by calcium and iron. There is considerable variation among the strains with regard to factors such as gr...
O Schmidt, U Moreth-Kebernik


Soft-rot in Tabebuia sp. wood used in water cooling tower: identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius


rDNA-ITS sequence of Serpula lacrymans and other important indoor rot fungi and taxon-specific priming PCR for their detection
1999 - IRG/WP 99-10298
Taxon-specific priming polymerase chain reaction (TSPP) is a powerful molecular tool for fungal diagnosis. For its application to indoor rot fungi, the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) of the main fungal species causing wood rot in European buildings was amplified with the polymerase chain reaction (PCR). The ITS region was sequenced. The complete sequences are present...
O Schmidt, U Moreth


Method of embedding and staining of wood after biological testing to support the identification of decay type
1998 - IRG/WP 98-20131
A method for preparing wood for microscopic analysis after decay testing is described, with special regard to a novel fast simultaneous staining, which helps to identify different types of decay. The novel staining process is explained as well as the embedding of heavily decayed samples in polyethylene glycol (PEG) and picking up of sections by transparent adhesive tape. Special feature of the int...
A O Rapp


The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


Assessment of decay risk of airborne wood-decay fungi
2012 - IRG/WP 12-10787
The decay risk of airborne wood-decay fungi was investigated by using an air sampler. Japanese cedar disks measuring about 8 cm in diameter and 3 mm in thickness with moisture content at about 100 % were placed in a “BIOSAMP” air sampler and exposed to 1000 liters of air. Air sampling was carried out from June to September at the same sampling site in Tsukuba, Japan. The exposed disks were the...
I Momohara, Y Ota, K Sotome, T Nishimura


Identification of soft-rot fungi existed in the samples from the galley excavated at Yenikapi
2014 - IRG/WP 14-10833
The shipwrecks of the Middle Byzantine period were excavated during the construction of the Marmaray railway and metro station in Yenikapı between 2004 and 2012. This has become the largest investigations because of the size and the number of the shipwrecks and its associated artifacts. In the previous report, the electron micrographs of the samples revealed that a wide range in the degree of...
T Wada, C Köse, K Igarashi


Data sheet on wood-boring insects. Apate monachus Fabricius. 2. Position systématique, nomenclature, identification et distribution - Espèces végétales attaquée
1981 - IRG/WP 1105
R L A Damoiseau


Microbiological degradation of wooden piles in building foundations
1988 - IRG/WP 1370
White rot, soft rot and bacterial attack have been detected in softwood piles under buildings. In some cases bacteria were found to be the main degradation organisms in the studied piles. The water content of degraded piles was very high. The compression strength was quite low also in the piles deteriorated by bacteria. The density of wood was very variable, and the degree of degradation could not...
L Paajanen, H Viitanen


Effect of acetylation on decay resistance of wood against brown-rot, white-rot and soft-rot fungi
1989 - IRG/WP 3540
Effect of acetylation on decay resistance of wood was investigated using wood blocks of Cryptomeria japonica, Pinus densiflora, Albizia falcata and Fagus crenata. Blocks were treated with uncatalyzed acetic anhydride for different lengths of time and exposed to Tyromyces palustris, Serpula lacrymans, Coriolus versicolor and unsterilized soil. The action of OH-radical on acetylated wood was also ex...
M Takahashi, Y Imamura, M Tanahashi


A comparison of soft rot, white rot and brown rot in CCA, CCP, CCF, CCB, TCMTB and benzalkonium chloride treated Pinus radiata IUFRO stakes, after 9-15 years exposure at five test sites in New Zealand
1991 - IRG/WP 1485
The aim of this study was to determine if decay type varies significantly between five field trial test sites of different soil type, aspect and climate in 9-15 year old, replicate CCA, CCF, CCP. CCB, TCMTB and AAC treated IUFRO stakes. A visual on-site assessment of decay type on every test stake was made and observations confirmed by microscopical examination. Regression analyses were used to de...
R N Wakeling


Questionnaire - Fungal decay types
1985 - IRG/WP 1265
T Nilsson


Collaborative soft rot tests. Names and addresses of collaborators
1973 - IRG/WP 231
IRG Secretariat


Penichroa fasciata (Stephens) (Col. Cerambycidae) a pest in wood materials
1988 - IRG/WP 1365
Penichroa fasciata (Stephens) (Col. Cerambycidae) is found to be a frequent pest occurring in hardwood in storage in Italy. This paper reports the characteristic for identification, biological features, distribution and timber liable to attack....
A Gambetta, E Orlandi.


Soft rot penetration - Effect of groundline maintenance treatment on poles in sevice
1983 - IRG/WP 3263
R S Johnstone


Improved techniques designed for evaluation of fungicides in soil for control of dry rot fungus Serpula lacrymans
1985 - IRG/WP 2238
Improved techniques provide a laboratory method for the evaluation of chemicals in soil for control of dry rot fungus Serpula lacrymans. Results with their application to three chemicals were reported. These techniques are useful to eliminate chemicals lacking the necessary toxicity and weatherbility for dry rot control when the chemicals have been applied to the soil....
M Takahashi, K Nishimoto


Collaborative soft rot tests: PRL tests of Cu/Cr/As preservative using method of Document No: IRG/WP/208
1973 - IRG/WP 223
These tests were undertaken as a preliminary to the next series of collaborative soft rot tests. An interim report has already been presented at Berlin in 1972 as Document No: IRG/WP/211...
J K Carey, J G Savory


The restricted distribution of Serpula lacrymans in Australian buildings
1989 - IRG/WP 1382
Temperature data has been gathered over a number of years, not only for flooring regions of various buildings in Melbourne, but also within roof spaces and external to the buildings. Findings are discussed in relation to the distribution of Serpula lacrymans within Australia, its restriction to certain types of building construction and its restriction to flooring regions. The subfloor spaces of b...
J D Thornton


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