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Characterisation of growth and stain of different groups of sapstain fungi on lodgepole pine
1999 - IRG/WP 99-10326
Canada is the world's largest exporter of softwood lumber. These softwood shipments are susceptible to a variety of wood-inhabiting fungi that can lead to sapstain discolouration, which in turn decrease the product value. Furthermore, the presence of these microorganisms may be unacceptable to the importing countries. The objective of this work is to assess the sapstaining capability and ...
C Fleet, C Breuil, A Uzunovic, A Byrne


Characteristics of the pigments produced by sap-staining fungi
1997 - IRG/WP 97-10223
The present study was conducted to characterize the pigments produced by sap-staining fungi, Aureobasidium pullulans (de Bary) Arnaud and Ceratocystis piceae (Münch) H. and P. Sydow. The pigments isolated from tested fungi were identified as melanins by spectroscopic methods (UV and infrared spectra). The UV and the infrared spectra of the fungal pigments were similar to those of the synthetic me...
M Mori, M Takahashi


Growth inhibitory effects on blue-stain fungi of applied electricity fields
1996 - IRG/WP 96-10167
Exploratory laboratory experiments on the effects of electricity on two blue stain fungi Aureobasidium pullulans and Ceratocystis piceae on wood revealed that a potential gradient of 1 V/cm corresponding to a current of 15 mA (DC), applied without interruption during a 2 week experimental period, leads to an inhibition of the growth of these fungi. Germination is somewhat more sensitive than mycel...
J Bjurman


Comparison of bluestain fungi growing in vitro and in vivo
1996 - IRG/WP 96-10149
Both moulds and bluestain fungi cause serious economic losses for forestry and timber processing industries and much research is aimed at finding environmentally and economically acceptable methods of control. It is especially important to study the growth of these fungi in freshly cut wood, which has been unaltered by drying or sterilisation, and which therefore resembles the substratum they woul...
A Uzunovic, J F Webber, D J Dickinson


Blue-stain fungi (Ceratocystis spp.) found in Spain on pine woods
1989 - IRG/WP 1410
So far, there is only a very limited reported description of the different Ceratocystis spp. present on fresh wood in Spain. So, the main goal of this work has been the identification of species of this genus causing blue-stain on Pinus pinaster A. Ait. and Pinus sylvestris L. woods. We have also investigated the relationship between the species found and their propagation vectors (insects and win...
M T De Troya, A M Navarrete


Detection of semi-quantitative and qualitative enzymatic activities of blue-stain fungi
2000 - IRG/WP 00-10347
Blue-stain, produced in forests, continues to be a problem in countries of moderate climate. In forestry, in certain geographical areas of Spain, it has been observed that anti-sapstain products have not always been efficient, as their fungicidal effectiveness varies on occasion, depending on the species of wood and microbiota that exist in the region. It is not always easy to identify the species...
M T De Troya, F Llinares, D Muñoz-Mingarro, M J Pozuelo, N Acero, C Rodríguez-Borrajo, A M Navarrete


Laboratory fumigations to determine the minimum temperature for methyl bromide eradication of the oak wilt fungus in red oak
1983 - IRG/WP 3243
Laboratory chamber fumigations of naturally-infected ret oak log sections exterminatet the oak wilt fungus in sapwood at temperatures down to 0°C. Only low, sporadic fungus survival was observed when log sections were gassed with methyl bromide at -5°C and aired for 4 da. at 0°C. Lower temperature treatments were not effective even if gas levels or exposure times were increased by 50%....
E L Schmidt


The use of chlorothalonil for protection against mold and sapstain fungi. Part 1: Laboratory evaluation
1989 - IRG/WP 3515
Laboratory screening of chlorothalonil alone and in combination with other fungicides was conducted against six mold and sapstain fungi. The most promising treatments appear to be chlorothalonil supplemented with CCA or copper-8-quinolinolate. Field tests have been implemented....
J A Micales, T L Highley, A L Richter


Copper-resistant fungi on pressure impregnated wood in Denmark
1994 - IRG/WP 94-10078
The occurence of Amorphotheca resinae Parbery and its asexual stage Cladosporium resinae (Lindau) de Vries on CCA and CCB treated wood has previously been shown. In the autumn 1993 some other blue stain fungi were found on CCP and CCB treated pine timber, such as Ophiostoma minus (Hedgc.) H. and P. Sydow, Ophiostoma pilifera (Fr.) H. and P. Sydow and Ophiostona piceae (Munch) H. and P. Sydow. The ...
J Bech-Andersen, S A Elborne


The use of immunofluorescence labelling for detecting Ophiostoma piceae in radiata pine
1998 - IRG/WP 98-10268
The primary objective of the current investigation is to understand fungal interactions of dominant sapstaining fungi in radiata pine using confocal laser scanning microscopy (CLSM). In the current paper, an immunofluorescence labelling technique was developed for detection of Ophiostoma piceae using a monoclonal antibody. The primary antibody was labelled with Oregon green 514 Goat anti-mouse Ig...
Ying Xiao, B Kreber, C Breuil


Methyl bromide eradication of the oak wilt fungus in logs. Laboratory and field fumigation
1981 - IRG/WP 3168
Concern over accidental introduction of the oak wilt fungus (Ceratocystis fagacearum) into oak-importing nations has prompted a study supported by the U.S. National Lumber Exporter's Assn. to assess the efficacy of methyl bromide fumigation to eradicate the fungus from logs and lumber. Laboratory and field fumigation trials to develop a reliable and realistic treatment were performed on r...
E L Schmidt, M M Ruetze, D W French


Biological control of sapstain fungi in wood
1996 - IRG/WP 96-10158
Sapstain fungi can cause serious damage to wood and wood products, resulting in a significant economic loss for the wood products industry. The purpose of the present investigation was to determine whether the sapstain in wood caused by sapstain fungi could be biologically controlled. Biological control of sapstain fungi in wood was demonstrated in field trials with nonpigmented isolates of Cerato...
S C Croan


Laboratory evaluation of chlorothalonil formulation for stain and mold control on rubberwood and maple
1998 - IRG/WP 98-30175
We evaluated the efficacy of several chlorothalonil and carbendazim fungicides (F1 and F2), etc. in the control of mold and stain fungi on rubberwood and maple. The results showed that these formulations effectively inhibited the selected fungal species such as Aspergillus niger, Penicillium sp., Trichoderma sp. (P71H), Aureobasidium pullulans, Ceratocystis minor (C-188), Ceratocystis pilifera (RW...
Mingliang Jiang, T L Highley, L Ferge, T L Woods


Using DNA probes to characterize the metabolic pathway of pigment production in several wood-staining fungi
1996 - IRG/WP 96-10146
During shipment and storage, lumber is susceptible to sapstain, a wood discoloration caused by fungi. Currently kiln drying and chemical applications are used to control sapstain. However, the chemicals used to protect wood have a broad range of action, and so can affect other organisms. In addition, in Canada most of these chemicals are under temporary registration. Thus there is a need to develo...
R Eagen, S Riecken, J Kronstad, C Breuil


Controlling the sapstain fungus Ceratocystis coerulescens by metabolites obtained from Bjerkandera adusta and Talaromyces flavus
1993 - IRG/WP 93-10024
Sapstain causes severe damage to wood and wood products, posing a major economic problem for the wood industry. The purpose of this study was to determine if metabolites from Bjerkandera adusta and Talaromyces flavus would (1) decolorize stain in wood caused by Ceratocystis coerulescens and (2) prevent sapstain by Ceratocystis coerulescens. We studied the interaction of the sapstain fungus Ceratoc...
S C Croan, T L Highley


Laboratory screening to determine the preventive effectiveness against blue stain fungi and moulds
1991 - IRG/WP 3677
This paper reports results of preservative treatment and leaching experiments, using borax, polybor and boracol 20, on small wood blocks of English oak and American pitch pine heartwood. Earlier experiments on the performance of various biocides as possible additives to bilgewater to prevent fungal decay of shipping timbers had suggested that some formulations of boron might be associated with phy...
M T De Troya, A M Navarrete


Localized induction of hemlock brownstain by Ophiostoma piceae
1995 - IRG/WP 95-10106
Hemlock brownstain, a coloration disfiguring western hemlock and amabilis fir, causes economic loss in the high-value Canadian export lumber market. Recent work by the author has suggested that wood-sapstaining fungi can induce the formation of brownstain. Ophiostoma piceae, the most frequent staining fungus on western hemlock lumber in B.C., was chosen as a model to investigate fungal participati...
B Kreber


Destaining wood sapstains caused by Ceratocystis coerulescens
1996 - IRG/WP 96-10159
Fungal sapstain does not reduce the strength of wood, but it does discolor the wood, detracting from its appearance and decreasing the value of wood and wood products. The purpose of this investigation was to assess whether wood sapstain caused by Ceratocystis coerulescens could be destained and existing growth eradicated. The hydroxyl radicals generation under optimized conditions destained the s...
S C Croan


Application of a novel strength evaluation technique during screening of wood preservatives
1986 - IRG/WP 2262
The effectiveness of CCA and ACA in treated aspen mini stakes tested using a novel bag procedure, with unsterile soil fortified with Chaetomium globosum and Ceratocystis albida, is reported. Good agreement between toxic limits determined using the standard weight loss procedure, and those determined by the strength technique were found, with some indication that the strength loss method is more se...
J N R Ruddick


Differential susceptibility of living and dead timber to colonisation by sapstain and mould fungi
1998 - IRG/WP 98-10288
Field studies have revealed that when timber is irradiated (i.e. killed) it is more susceptible to colonisation by mould fungi than sapstain fungi. By comparison, freshly sawn timber shows very little mould colonisation, tending to be colonised by sapstain fungi. It appears, therefore that the physiological state of the wood may influence the pattern of colonisation. A laboratory trial was underta...
J R Williams, D J Dickinson, J F Webber


Laboratory and field trials of novel antisapstain formulations
1997 - IRG/WP 97-30146
This document covers the results of laboratory and field trials of combinations of fungicides formulated using a patented technology (PCT NZ 96/00143). A 3 week laboratory trial that uses radiata pine (Pinus radiata D. Don) branch discs as a substrate was used to determine which combinations possessed a suitable spectrum of fungitoxicity against key sapstain, mould and decay fungi. In particular f...
R N Wakeling, P N Maynard, D R Eden, C M Chittenden, B Carpenter


Comparison between two laboratory test methods for determining the effectiveness on wood preservatives against blue stain in fresh wood
1987 - IRG/WP 2289
Most of the work done on determination of the effectiveness of new formulae for treating fresh wood against blue stain have been focussed on their use in the manufacture of saw timber. This work explains two laboratory methods, one which simulates the working and climatological conditions of factories making packages for fruit and vegetables in the Spanish Levante, showing that contamination of wo...
A M Navarrete, M T De Troya


Growth of sapstain fungi in scots pine and the effect of timber ageing
1998 - IRG/WP 98-10269
A trial was initiated to study the growth of known isolates of sapstain fungi in scots pine (Pinus sylvestris) which had been naturally aged or sterilised by autoclaving or irradiation. Sawlogs cut from commercial thinnings were reduced to billet size and the exposed ends were painted with a bituminous sealant to reduce moisture loss. Some billets were inoculated after autoclaving or gamma-irradia...
N J Strong, J F Webber, R A Eaton


Development of a disinfection treatment for oak logs to be imported from the USA
1984 - IRG/WP 3283
The European veneer industry depends greatly on oak supplies from the USA. To prevent the accidental introduction of the American Oak Wilt Disease (Ceratocystis fagacearum) into the member states of the EC, a disinfection treatment was developed for oak logs under consideration of the technical requirements of veneer production. Laboratory experiments and field trials in Germany and in the USA sho...
W Liese, M M Ruetze


Effect of thickened boron in preventing conidial germination of sapwood-inhabiting fungi
1994 - IRG/WP 94-30059
We evaluated the efficacy of thickened boron preservative "Diffusolä" in preventing conidia germination of sapwood-inhabiting fungi using plate bioassay, Southern Yellow Pine and sweetgum block tests, and green pine log sections. The test fungi were sapstain fungi Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans and mold fungi, Aspergillus niger, Penicillium spp, and Tric...
S C Croan


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