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Improvements of monitoring the effects of soil organisms on wood in fungal cellar tests
1996 - IRG/WP 96-20093
Accelerated testing the durability of preservative treated timber in a so called "fungal cellar" or "soil-bed" to evaluate its performance in ground contact is widespread practice. In order to obtain a more accurate and reproducible estimate of preservative performance, several institutes, among them the BAM in Berlin, have routinely carried out static bending tests in addition to visual examination. These tests were usually performed with a defined maximum load or deflection path regardless of the remaining degree of elasticity of the test specimens. Recent studies at the BAM revealed that by modifying the method, i.e. by restricting the applied load to the non-destructive interval for each individual test specimen, the calculated modulus of elasticity (MOE) reflect the changing strength properties caused by biological deterioration and allow within a relatively short time valuable predictions on the service life of the treated timber in soil contact.
I Stephan, S Göller, D Rudolph


Japan's comments on ISO/DIS 12583-1/2
1996 - IRG/WP 96-20100
The paper describes an accelerated field test for the evaluation of timber preservative formulations against subterranean termites. The method has been adopted by the South African wood preservation industry as a screening method for the approval of wood preservatives for use under SA conditions. The method which is based upon the fungal cellar test offers a rapid means of evaluating the comparative performance of new wood preservative formulations in an environment that accurately reflects field conditions.
P Turner, D Conradie


Protocol for evaluation and approving new wood preservative
1985 - IRG/WP 2159
M E Hedley, J A Butcher


Performance of Tebbacop in laboratory, fungus cellar and field tests
2000 - IRG/WP 00-30222
A novel organo-copper wood preservative ("Tebbacop") has been exposed to 12 years fungus cellar testing and 6 years above ground field testing. In the fungus cellar, Tebbacop at a retention of 0.053% Cu m/m oven dry wood out-performed CCA at a retention of 0.55% m/m oven dry wood. In above ground tests, L-joints treated to a Tebbacop retention of 0.012% Cu are performing as well as joints treated with TBTN to a retention of 0.08% Sn. In a slightly more severe decking test, 0.016% Cu as Tebbacop was more effective than 0.08% Sn as TBTN.
M E Hedley, D R Page, B E Patterson


Are fungal cellar tests really necessary?
1989 - IRG/WP 2333
During the past decade the range of methodology used to evaluate wood preservative potential has significantly expanded. At the forefront of these new tools available to the scientist·is the fungal cellar. This technique, as currently applied, involves the exposure of treated and untreated samples to conditions of moisture and temperature which ensure optimum fungal attack. By comparison data with that obtained on similar preservative systems in conventional stake tests, acceleration factors for predicting performance have been developed. This discussion paper examines the philosophy influencing the development of fungal cellar testing, and provides an alternative viewpoint.
J N R Ruddick


Fungus cellar and stake tests with tall oil derivatives. Progress Report after 5 years' testing
1987 - IRG/WP 3442
Two derivatives of tall oils have been tested for five years in fungus cellar and stake tests. The samples were relatively quickly attacked on the surface by decay fungi, mainly soft rot, but the decay has progressed very slowly. The performance of the stakes in the tests has so far been equivalent or even better than some CCA preservatives and creosote.
J Jermer, Ö Bergman, T Nilsson


Comparison of decay rates of preservative-treated stakes in field and fungus cellar tests
1980 - IRG/WP 2135
With the exception of acid-copper-chromate, zinc-chrome-arsenate, and sodium pentachlorophenoxide, the relative performance of preservatives in the fungus cellar was similar to that in the field.
M E Hedley


Questionnaire: Facility for accelerated stake tests in unsterile soil
1981 - IRG/WP 2166
An acceptable name for this type of test facility has not yet been devised, but it has previously been referred to as a FUNGUS CELLAR or as a TROPICAL DECAY HOUSE. The attached pamphlet (What's New in Forest Research No.65) describes in broad terms the facility used for this purpose at FRI in New Zealand. Over the last two or three years several laboratories and commercial firms have installed so-called fungus cellars. At the 1981 I.R.G. Meeting in Sarajevo it was proposed that a survey be undertaken to obtain basic information on the various developments in this new area of biological testing. To this end, I shall be pleased if you complete the enclosed questionnaire and return it to: Dr J.A. Butcher, Forest Research Institute, Private Bag, Rotorua, NEW ZEALAND. A full report on this survey will be presented at the next I.R.G. meeting scheduled for Turkey in May 1982.
J A Butcher


Report on questionnaire: Facility for accelerated stake tests in unsterile soil
1983 - IRG/WP 2169
In October 1981 a questionnaire (IRG Doc. No. IRG/WP/2166) on the so-called fungus cellar tests in unsterile soil was prepared and despatched to 56 individuals representing various institutes, organisations, or companies. In addition a further 68 letters describing the purpose of the questionnaire were sent to additional IRG members who could request the full questionnaire if appropriate. This represented a contact with 125 individuals in 48 countries. A verbal report on the results of this survey was presented at IRG 13 in Cesme, Turkey. The present document formalises that report.
J A Butcher


Comparison of decay rates of preservative-treated stakes in field and fungus cellar tests. Results after 40 months fungal cellar exposure
1983 - IRG/WP 2200
Decay rates of preservative-treated Pinus radiata stakes during 40 months exposure in the FRI fungus cellar were compared with those of similarly treated material in a field test. Decay rates in the fungus cellar were from 4 to 100 times higher than in the field, although for the majority of preservatives the rate was between 7 and 12 times higher. The lag phase before onset of decay, noticeable with most of the preservatives in the field test, was largely eliminated in the fungus cellar. Possible reasons are given for inconsistencies in relative rates of decay of preservatives in the two tests.
M E Hedley


Fungus cellar and field tests with tall oil derivatives. Final report after 11 years' testing
1993 - IRG/WP 93-30007
Two derivatives of tall oil were tested as wood preservatives in a fungus cellar and in ground contact (field test). Stakes of Pinus sylvestris sapwood were used in the tests. For the field test the size of the stakes was 20 x 50 x 500 mm³ and for the fungus cellar test 20 x 20 x 250 mm³. The stakes were vacuum-pressure treated with the two products and exposed in 1981. The field test site used was in Uppsala, where the soil type is clay. In 1991 the last stakes in the field test were rejected and in 1992, the last stakes in the fungus cellar failed. The effect against biological degradation of the two products is compared with that of wood preservatives in current use.
J Jermer, Ö Bergman, T Nilsson


Assessment of the toxicity of some copper-, zinc- and boron-based wood preservatives to the cellar fungus Coniophora cerebella Schröet
1974 - IRG/WP 242
This article reports the use of a method based on the determination of the probability of the protection of timber against destruction by fungi. By converting the probability values to probit values and plotting them as a function of the amount of preservative retained in the timber, curves of the toxic effect are obtained, enabling any timber protection probability to be assessed.
V N Sozonova, D A Belenkov


Observations on the performance of copper-based wood preservatives in fungal cellar (soil-bed) tests
1994 - IRG/WP 94-20047
Fungal cellar (soil-bed) tests are considered to be an important tool for the evaluation of the performance of ground contact wood preservatives. Facilities of this type have been established world wide although caution has been exercised in their introduction into standard testing methods for the approval of wood preservatives. This is the result of concerns over the variability in the biological activity between different facilities, and thereafter the determination of effective preservative retentions. This paper presents results of tests on copper based wood preservatives from four different fungal cellar facilities. The results show consistent trends in preservative performance and the high decay rates demonstrate the value of this type of test in determining the potential of new wood preservatives for long term protection in ground contact.
G R Williams, D Rudolph, M E Hedley, J A Drysdale, R F Fox


Some Experiences with Stake Tests at BAM Test Fields and in the BAM Fungus Cellar Part 2: Comparison of Static and Dynamic Moduli of Elasticity (MOE)
2005 - IRG/WP 05-20320
In routine in-ground stake tests of wood preservatives at the BAM test field Horstwalde and in the BAM fungus cellar periodical determinations of both static moduli of elasticity (MOE) on the basis of bending tests and dynamic MOE on the basis of the vibration method were performed as a possible method for the assessment of fungal attack. As expected from other publications, the absolute values of the dynamic MOE were higher than those of the static MOE. With the equation used for the calculation of the dynamic MOE, the differences were greater with larger specimen sizes. Sufficient correlation was observed, regarding the course of both MOE types within the test period. For both types of MOE, wood moisture contents of the stakes above the fibre saturation point were a prerequisite for the comparability of results in succeeding determinations.
M Grinda, S Göller


Tests with ammoniacal copper and alkyl ammonium compounds as wood preservatives
1984 - IRG/WP 3299
Formulations based on copper and alkyl ammonium compounds in ammonia solution have been tested in a fungus cellar on Pinus radiata and Fagus sylvatica. This type of products gives promising results as wood preservatives, especially on hardwood and are safe to destroy by e.g. combustion. The best results were achieved with a dialkyl ammonium compound, Cu/octyldecyl dimethyl ammonium chloride (NH3). The optimal weight ratio of Cu/AAC is for Pinus radiata = 0.2-0.4 and Fagus sylvatica = 1.0 based on cost-effectiveness. Fixation and leaching of coppertetrammine are discussed in detail. The leaching of active components from the Cu/AAC/NH3-systems is very low.
C-E Sundman


Effect of treatment process on performance of copper-chrome-arsenate. Part 1: Fungus cellar tests
1995 - IRG/WP 95-40045
Pinus radiata sapwood stakes 20 x 20 x 400 mm³ were treated with CCA Type C using a range of concentrations and three treatment processes; Bethell, Lowry and Rueping. Preservative retention was determined by chemical analysis of treated material. Following fixation, fungus cellar stakelets, 5 x 10 x 180 mm³ were cut from treated material and after leaching were exposed in unsterile soil beds in the FRI Fungus Cellar. Results after 65 months' exposure indicate that performance was related only to preservative retention. Differences in treatment process had little effect on decay resistance. This is in contrast to results from pure culture decay tests, reported earlier, which indicated that disproportionation of preservative elements using empty cell processes and low preservative concentrations had significant effect on durability.
M E Hedley, J Anderson, J B Foster, B E Patterson


Depletion of preservatives from treated wood: Results from laboratory, fungus cellar and field test
1993 - IRG/WP 93-50001-07
This paper compares results of preservative component losses using a range of test procedures with several different water-borne preservatives. The data shows that laboratory leaching tests are in some cases comparable to, but in other cases significantly underestimate preservative component losses when compared with field or fungus cellar depletion tests. The results also suggest that preservative component losses are treatment retention and formulation dependent. It is apparent that the changing nature of wood preservative formulations, coupled with the different types of treated commodities and applications now being used, mandates the use of a wider range of test methods with multiple retentions to comprehensively assess likely loss of wood preservative components in service. The paper addresses a number of these issues and also presents test methods which may be suitable for further development in the area of preservative depletion.
L Jin, A F Preston


Some Experiences with Stake Tests at BAM Test Fields and in the BAM Fungus Cellar Part 1: Comparison of Results of Visual Assessments and Determinations of Static Moduli of Elasticity (MOE)
2005 - IRG/WP 05-20319
With examples of routine in-ground stake tests differences are shown in the performance of wood preservatives at the BAM test fields Lehre and Horstwalde and in the BAM fungus cellar. Signs of attack of micro-organisms were assessed visually according to EN 252. Periodical determinations of static moduli of elasticity (MOE) revealed the influence of the attack on the elastic properties of the wood specimens. The course of visible signs of attack and the residual MOE corresponded sufficiently in the fungus cellar. At comparable decrease rates of the MOE the visible signs of fungal attack developed slower at the test fields than in the fungus cellar. The stake dimensions distinctly influenced the accelerating effect of the fungus cellar.
M Grinda, S Göller


Efficacy of a novel copper-based organic solvent preservative in laboratory and fungus cellar tests
1995 - IRG/WP 95-30071
The efficacy of Chemicca 4, a novel copper-based organic solvent preservative, was compared with that of other LOSP in pure culture laboratory decay tests and with that of CCA and TBTO in a fungus cellar exposure. It was markedly superior to copper and zinc naphthenates at equivalent metal retentions in laboratory decay tests, and similar in activity to TBTO. After 60 months' fungus cellar exposure, Chemicca 4 at a retention of 0.03% mass/mass copper showed similar effectiveness to CCA at a retention of 0.31% mass/mass total active elements and was superior to TBTO at a retention of 0.09% mass/mass Sn. At 80 months' exposure Chemicca 4 at 0.16% mass/mass Cu was superior to CCA at 0.55% mass/mass total active elements. Extensive world-wide field testing of Chemicca 4 in above ground and ground contact environments both as an LOSP (above ground and ground contact) and in P9 oil (ground contact) is currently being undertaken.
M E Hedley, P N Maynard


The durability of acetylated radiata pine sapwood (Accoya). Results from five years testing
2011 - IRG/WP 11-30560
New Zealand grown radiata pine sapwood was modified using the Accsys PLC acetylation process (Accoya). Following treatment, various test samples were exposed in the Scion Accelerated Field Test facility (Fungus Cellar) and in exterior ground contact field tests. In the Fungus Cellar, performance was compared with that of radiata pine treated with CCA to Hazard Classes H3.2 (0.37% m/m Cu+Cr+As) and H4 (0.63 % m/m Cu+Cr+As) and with kwila heartwood (Intsia bijuga). In the field trial performance was again compared with that of H3.2 and H4 treated radiata pine and with the heartwood of the naturally durable species teak (Tectona grandis), western red cedar (Thuja plicata) and cypress (Cupressus macrocarpa). In both tests and after five years, the Accoya wood was in better condition than the CCA treated samples and the four naturally durable species.
M Hedley, D Page, J van der Waals, K Nasheri, G Durbin


Evaluation of new creosote formulations after extended exposures in fungal cellar tests and field plot tests
2000 - IRG/WP 00-30228
Although creosote, or coal tar creosote, has been the choice of preservative treatment for the railroad industry since the 1920s, exuding or "bleeding" on the surface of creosote-treated products has been one incentive for further enhancements in creosote production and utility (Crawford et al., 2000). To minimize this exuding problem, laboratories such as Koppers Industries Inc., USA, and Commonwealth Scientific and Industrial Research Organization (CSIRO), Division of Chemical and Wood Technology, Melbourne, Australia, have developed changes in processing of coal tar that produce distillates with fewer contaminants. This "clean distillate" is then used to formulate "clean creosote" as a preservative. These new, unique creosote formulations are being investigated as part of a program to enhance the use of regionally important wood species in the United States. Four retention levels of each of two new creosote formulations creosote, one pigment-emulsified creosote (PEC) and one creosote formulation that meets the AWPA Standard C2-95 for P1/P13 creosote (AWPA, 1995a), were applied to two softwood species and two hardwood species. Two laboratory procedures, the soil-block and fungal cellar tests (accelerated field simulator), were used to evaluate the four creosote formulations. These procedures characterized the effectiveness of the wood preservatives. The soil-block tests were used to determine the minimum threshold level of the preservative necessary to inhibit decay by pure cultures of decay fungi. In general, the soil block tests showed there was little difference in the ability of the four creosote formulations to prevent decay at the three highest retention levels as summarized in a previous report by Crawford and DeGroot (1996). The soil-block tests will not be discussed in this report. Fungal cellar tests expose treated wood to mixtures of soil-borne fungi that promote accelerated attack. Crawford and DeGroot (1996) discussed the evaluation of the creosote formulations after 17 months of exposure in the USDA Forest Service, Forest Products Laboratory (FPL), fungal cellar. At that point in time data from the fungal cellar tests showed that softwoods are protected better than hardwoods for all four formulations of creosote tested. This report will discuss exposure of the fungal cellar stakes upto 36 months. In addition, field stake tests are being used to verify service life of the new creosote formulations in vivo. Results from accelerated tests are indicative of field performance, but the correlation between laboratory and field results is still being investigated. Field stake tests are regarded as critical, long-term evaluations that provide results most directly related to the performance of treated products in service. In this study, we report on the performance of the creosote formulations after five years of exposure in field tests.
D M Crawford, P K Lebow, R C De Groot


The accelerated field simulator (= fungal cellar)
1982 - IRG/WP 2170
G C Johnson, J D Thornton, H Greaves


Comparison of Different Methods for Assessing the Performance of Preservatives in the BAM Fungus Cellar Test
1998 - IRG/WP 98-20149
The fungus cellar test is a common means to get reliable data on the long term performance of treated wood in soil contact. A constantly high humidity and a suitable of water holding capacity for a range of micro-organisms provide high decay rates in untreated wood and produce intensive microbial pressures on wood treated with biocides. Presently a range of biocides are under test in the BAM fungus cellar and the results will be presented for the following types of biocides: Tebuconazole in combination with copper and boron (5 years fungus cellar), quats with copper and boron (5 years fungus cellar) and Cu-organic compound combined with copper and boron (3 years fungus cellar). Figures will be shown on the development of the Modulus of Elasticity (MOE) over the years and on an assessment of the stakes according to EN 252.
I Stephan, M Grinda, D Rudolph


A combination of chlorothalonil and chlorpyrifos for more effective wood preservation
1995 - IRG/WP 95-30067
Chlorothalonil (tetrachloroisophthalonitrile) is a highly effective fungicide and wood preservative. Chlorpyrifos (O,O-diethyl O-(3,5,6-trichloro-2-pyridinyl)phosphorothioate) is an insecticide and is not generally considered to possess significant antifungal activity. However, a review of the literature revealed that chlorpyrifos effects in various ways the activity of microorganisms and is even registered for control of a fungal plant disease. Laboratory and field research trials were designed to study the wood preservative effectiveness of combinations of chlorothalonil plus chlorpyrifos. In some tests the antifungal activity of the insecticide chlorpyrifos was confirmed; however in a fungus cellar test, chlorpyrifos by itself provided little protection. In laboratory termite testing, chlorothalonil was sufficiently effective that in most experiments the addition of chlorpyrifos had little effect, while in field tests the combination consistently outperforms chlorothalonil alone. In tests against various marine borer species, the combination of chlorothalonil plus chlorpyrifos proved superior to chlorothalonil alone. The synergy of chlorothalonil plus chlorpyrifos is demonstrated in field stake tests, where after eleven years in Florida the combination has given superior performance over stakes treated with pentachlorophenol or chlorothalonil alone. Chlorpyrifos alone provided little protection against decay in these field stake tests. Chlorpyrifos is a useful additive to wood preservative fungicides, conferring additional protection against both decay fungi and wood destroying insects.
T L Woods, P E Laks, T C Blewett, R D Fears


Recent soft-rot research in softwoods and hardwoods
1980 - IRG/WP 1108
The purpose of this paper is to describe briefly the current status of our research on soft-rot fungi. The work to be discussed is still in progress and any results described must be regarded as provisional.
J A Butcher


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