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Inhibitory effects of leachates from Scots pine wood on germination of some wood rotting fungi
1986 - IRG/WP 1282
Leachates from sapwood of Pinus sylvestris inhibited or reduced the germination of basidiospores of the wood decay fungi tested. The fungi were selected among those preferentially colonizing hardwood or softwood, representatives for brown rot and white rot fungi, early basidiomycete colonizers and late basidiomycete colonizers in above-ground parts of pine as well as fungi found in high frequencie...
J Bjurman


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


Partial characterization of inhibitors extracted from pine (Pinus sylvestris) sapwood active against germination of wood rotting fungi
1988 - IRG/WP 1351
Germination inhibitors in pine sapwood could be extracted and separated with the aid of solvent partitioning and chromatography on silicic acid. The inhibitory action was tested by three different bioassays. Active fractions have been characterized by TLC and HPLC. Inhibitory activity could be correlated with phenolic compounds. The minimum active concentration of inhibitors have been determined....
J Bjurman


Synergistic effect of boron on Streptomyces rimosus metabolites in preventing conidial germination of sapstain and mold fungi
1992 - IRG/WP 92-1565
We evaluated the synergistic effect of boron (4% BAE solution of Tim-Bor or 4% boric acid) on Streptomyces rimosus metabolites in preventing spore germination of sapstain and mold fungi using plate bioassay, Southern yellow pine and sweetgum block tests, and green pine log sections: sapstain -- Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans; mold fungi -- Aspergillus nig...
S C Croan, T L Highley


Monographic cards for wood-destroying fungi. [Fiches monographiques pour les champignons lignivores]
1970 - IRG/WP I 5B
C Jacquiot


Successive collections of Basidiospores from wood decay fungi (in vitro) show variation in germination levels on common media
1978 - IRG/WP 191
In the course of various preliminary experiments in which spore germination levels of 6 decay fungi on malt and water agar were recorded as controls, it was noted that one could not reliably obtain an expected level of spore germination for any particular fungus. Inconsistent 'control' spore germination levels of a fungus greatly complicates large scale experiments in which compa...
E L Schmidt, D W French


Use of vermiculite as substrate in assays on phytotoxicity of treated wood
1989 - IRG/WP 3547
It is considered the possibility of using vermiculite instead of soil as substrate in assays on phytotoxicity of wood treated with preservatives for agricultural use. Three organic preservatives were used. It had been tested the behaviour of both, vermiculite and soil in case of preservative leaking due to a leach. So that, assays of germination with cucurbitaceous were carried out, mixing a dose ...
M V Baonza Merino, D Franco


Influence of aliphatic acids on spore germination of wood decay fungi
1984 - IRG/WP 2224
Influences of eight saturated fatty acids (C5-C10, C12 and C16) on spores of four isolates of wood decaying basidiomycetes (white rot fungi: Poria tenuis and Trametes hispida; brown rot fungus: Gloeophyllum trabeum [two isolates]) were observed in-vitro. Spore response after 24 hr on malt extract agar containing 10, 10² , or 10³ ppm of each fatty acid included: no effect on normal germination, d...
E L Schmidt


Effects of a chitin synthesis inhibitor on spore germination of the decay fungi Gloeophyllum trabeum and Poria tenuis
1986 - IRG/WP 2253
This study sought to determine the effect of a chitin synthesis inhibitor - Polyoxin D, on spore germination and early hyphal development of a brown rot and a white rot fungus in-vitro. Polyoxin D is a competitive, substrate-analogue type of chitin synthtase inhibitor (2). The drawbacks to use of such a compound as a wood preservative tie. cost - $20,000/g, possible leaching or degradation) might ...
E L Schmidt


Spore germination of Gloeophyllum trabeum on wood is related to the mass of the wood sample
1978 - IRG/WP 2118
E L Schmidt, D W French


Antagonism to spore germination in Scots pine
1990 - IRG/WP 1458
Germination of Lenzites trabea and Chaetomium globosum spores was observed directly on wood blocks, on water soluble extracts and on organic solvent soluble extracts from pine wood. In all cases pine heartwood was found to be antagonistic to spore germination but pine sapwood varied in its antagonism according to the method of drying. Chromatography revealed that extracts from antagonistic wood di...
S M Gray


Germination of basidiospores on preservative treated wood after leaching or natural weathering
1981 - IRG/WP 2150
In tests of residual toxic efficacy after leaching or natural weathering, spore germination with Gloeophyllum trabeum has proved to be a less reliable criterion of attack than when used with unaged preservative treatments. Since spores sometimes prove more tolerant than their parent mycelium, their use should be continued....
J K Carey


Effect of sterilization method on germination of spores of wood decay fungi observed by contact agar block method
1978 - IRG/WP 2117
Previous studies of germination of spores of wood decay fungi on wood have generally concluded that method of wood sterilization has little significant effect on germination response. This study expands the numbers of test fungi as well as number of sterilization methods employed to determine the influence of sterilization method on spore germination response of decay fungi. Germination was assess...
E L Schmidt, D W French


In vitro studies on the effect of chitosan on mycelial growth and spore germination of decay fungi, moulds and staining fungi
2004 - IRG/WP 04-10507
The effect of solubilised, low molecular weight chitosan on established mycelial growth of a range of decay fungi, moulds and staining fungi was investigated using nutrient medium amended with different concentrations of chitosan that ranged from 0.1 to 0.4% weight per volume (%w/v). Also, spore germination of Trichoderma harzianum and Leptographium procerum was examined on chitosan amended nutrie...
C Chittenden, B Kreber, N McDowell, T Singh


A rapid colorimetric assay for mold spore germination using XTT tetrazolium salt
2011 - IRG/WP 11-20462
A rapid colorimetric assay was developed to quantitate metabolic activity in mold spores during germination using 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[9phenyl-amino)carbonyl]-2H-tetrazolium hydroxide (XTT). The assay was used to demonstrate inhibition of spore germination following exposure to different biocides and variability in the inhibition of spore germination of different mold specie...
C A Clausen, V W Yang


The possible role of mobile CCA components in preventing spore germination in checked surfaces, in treated wood exposed above ground
2001 - IRG/WP 01-30263
Untreated check surfaces are often exposed in CCA-treated lumber of refractory species used above ground since, during weathering, some checks develop beyond the preservative penetrated zone. However, decay is seldom observed in these checks even after many years of exposure. It is hypothesized that minor amounts of mobile CCA preservative components redistribute during weathering into checks, and...
S Choi, J N R Ruddick, P I Morris


Effect of fumigant residue in aerated wood blocks on the spore germination of decay fungi
1991 - IRG/WP 2382
Fumigants are increasingly used in several countries for remedial treatments of transmission poles to increase the service life. The present study was initiated primarily to test the remaining toxic effects of spruce (Picea rubra) wood, fumigated with chloropicrin (trichloronitro methane) or MIT (Methyl isothiocyanate) after long period of aeration, on the spore germination of decay fungi. This st...
J Bjurman, B Goodell


How predictive are laboratory experiments for assessing the effects of chitin synthesis inhibitors (CSI) on field colonies of terrnites? - A comparison of laboratory and field data from Australian mound-building species of termit
1996 - IRG/WP 96-10143
A singular advantage of using mound building species of termite is their directly accessible nest. This allows evaluation of control methods by accurate assessment of the effects at the colony level. The mound building species Coptotermes acinaciformis (Froggatt) and Nasutitermes exitiosus (Hill) were used to test the CSI, hexaflumuron, in laboratory and field trials. Laboratory results showed tha...
M Lenz, P V Gleeson, L R Miller, H M Abbey


Preliminary studies of the performance of iron chelators as inhibitors of brown rot (Coniophora puteana) attack
1996 - IRG/WP 96-10185
This paper describes experiments to examine the proposal that the presence of iron is essential for brown rot fungi to utilize hydroxyl radicals remote from the hyphae as a means of converting the wood into a food source. reliminary test results are presented from trials using three different iron chelators impregnated into Scots pine (Pinus sylvestris) sapwood blocks. Their relative effects on th...
E D Suttie, R J Orsler, P M Wood


Effect of thickened boron in preventing conidial germination of sapwood-inhabiting fungi
1994 - IRG/WP 94-30059
We evaluated the efficacy of thickened boron preservative "Diffusolä" in preventing conidia germination of sapwood-inhabiting fungi using plate bioassay, Southern Yellow Pine and sweetgum block tests, and green pine log sections. The test fungi were sapstain fungi Ceratocystis coerulescens, Ceratocystis minor, and Aureobasidum pullulans and mold fungi, Aspergillus niger, Penicillium spp, and Tric...
S C Croan


Basidiospore structure and germination of Serpula lacrymans and Coniophora puteana
1988 - IRG/WP 1340
Using a nuclear staining technique and fluorescence microscopy, the basidiospores of Serpula lacrymans were shown to be uninucleate whereas those of Coniophora puteana were binucleate. The germination rate of the Serpula lacrymans spores, which was considerably lower than that of Coniophora puteana, decreased further after storage or heat treatment. Transmission electron micrographs indicate that ...
B M Hegarty, U Schmitt


The ecotoxicology assessment of wood preservatives and their active ingredients by means of germination tests using cress - A critical consideration
1999 - IRG/WP 99-50125
With putting the Biocidal Products Directive 98/08/EC (BPD) in place an environmental risk assessment for wood preservatives and impregnated timber is requested. To assess possible risks, suitable test methods are required, which reveal the ecotoxicological profile including environmental fate and behaviour of treated commodities. Germination and growth tests could contribute to the determination ...
P Jüngel, A O Rapp, E Melcher


Basidiospore germination threshold against borate in-vitro may vary between liquid and agar media
1992 - IRG/WP 92-2405
The concentration of disodium octaborate (BAE) which prevented mycelial growth of a common brown rot and 3 white rot fungi was compared to that required to prevent basidiospore germination on malt extract agar. Spore germination sensitivity was lower (50%) for only one of the white rot fungi. However, when spore germination thresholds were tested in malt extract-borate solutions in well slides (no...
E L Schmidt, Ya-Lih Lin


The growth and metal content of plants grown in soil contaminated by a copper/chrome/arsenic wood preservative
1977 - IRG/WP 3110
Salts of copper, chromium and arsenic are used together in water soluble formulations for the preservation of wood against insect and fungal attack. Copper/chrome/arsenic (CCA) preservatives are of proven efficacy and, used correctly, ensure a useful service life for timber for 30 years or more with little, if any, attendant threat to the environment from the treated wood itself. The preservative ...
C Grant, A J Dobbs


A microscopic study on the effect of IPBC/DDAC on growth morphology of the sapstaining fungus Ophiostoma piceae
1997 - IRG/WP 97-10191
An exploratory study was performed to probe into fungal/wood/fungicide interactions using Ophiostoma piceae, a common sapstaining fungus on unseasoned radiate pine. Treated (1% solution containing IPBC (0.07% w/w) and DDAC (0.60% w/w)) and untreated radiate pine wafers were inoculated with O. piceae and examined microscopically over a ten days incubation time. The results of the study showed that ...
Ying Xiao, B Kreber


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