Your search resulted in 13 documents.
Immunogold labelling of size marker proteins in brown rot-degraded pine wood
1990 - IRG/WP 1428
Pine wood degraded by Fomitopsis pinicola was infiltrated with a mixture of ovalbumin (45 kDa) and myoglobin (16.7 kDa). After crosslinking of the proteins with glutaraldehyde and preparation for electron microscopy ultrathin sections were labeled with gold-conjugated antibodies. Simultaneous labeling of both proteins on the same section showed that at 50-70% weight loss ovalbumin did not penetrat...
E Srebotnik, K Messner
Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola
1988 - IRG/WP 1343
By applying immunoelectronmicroscopic methods, lignin peroxidase of the white rot fungus Phanerochaete chrysosporium has been localized in the cytoplasm of hyphae, close to the plasmalemma and on the plasmalemma. Infiltration of wood specimen with culture filtrates, concentrated 300-fold, gave clear information on the penetration of the enzyme into the wood cell wall. Penetration was restricted to...
E Srebotnik, K Messner
Immunolocalization of xylanase produced by Clostridium xylanolyticum Rogers and Baecker during anaerobic decay of Pinus patula
1991 - IRG/WP 1505
The xylanolytic fraction from a 72h culture of Clostridium xylanolyticum ROGERS and BAECKER was separated using non-denaturing PAGE and then used to raise polyvalent antisera in rabbits. Antibodies were purified using an affinity gel column and coniugated with colloidal gold. Pinus patula was exposed to axenic cultures of Clostridium xylanolyticum for 3 weeks, fixed, embedded and converted to ultr...
A A W Baecker, G M Rogers
Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley
Wood Preservation in France. A statement of quality control early 1986
1986 - IRG/WP 3389
A statement of quality control in France early 1986 - Summary of new - Standards criteria for preservatives and treated wood - Aptitude of treated wood for use per class of biological hazard...
M Romeis, G Ozanne
Regulations of pesticides (including wood preservatives) in the United States
1977 - IRG/WP 397
G B Fahlstrom
Immunogold localisation of catalytic domain of endocellulase in the cellulosic substrates
1997 - IRG/WP 97-10205
The reaction of catalytic domain of endocellulase on the cellulosic substrates was visualised by using immunogold labelling procedures to obtain a better understanding of the mechanism of enzymatic cellulose hydrolysis. Polyclonal antibody was produced against the catalytic domain of endo-l, 4-ß-glucanase (EGI) from a ruminal anaerobic bacterium Ruminococcus albus F-40. Immuno-TEM works showed th...
Yoon Soo Kim, Seung-Gon Wi, Kyu Ho Myung, K Ohmiya, S Karita
The use of immunofluorescence labelling for detecting Ophiostoma piceae in radiata pine
1998 - IRG/WP 98-10268
The primary objective of the current investigation is to understand fungal interactions of dominant sapstaining fungi in radiata pine using confocal laser scanning microscopy (CLSM).
In the current paper, an immunofluorescence labelling technique was developed for detection of Ophiostoma piceae using a monoclonal antibody. The primary antibody was labelled with Oregon green 514 Goat anti-mouse Ig...
Ying Xiao, B Kreber, C Breuil
Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner
Immuno-electron microscopic localization of extracellular metabolites in spruce wood decayed by brown-rot fungus Postia placenta
1990 - IRG/WP 1441
Degradation by Postia placenta in spruce and birch wood was shown to occur not only in the wood cell wall but also in the middle lamellae region. Middle lamellae was often found to be degraded along the centerline so that cells could separate along this line. Extracellular membrane structures were found surrounding the hyphae and this matrix labelled positively with antisera produced to Postia pla...
Y S Kim, B Goodell, J Jellison
Wood preservatives. The state of French legislation (June 1977)
1977 - IRG/WP 3106
There does not exist any special legislation for preservatives but general laws may intervene at three levels:-Manufacture -Transport -Selling. In every case, it is based on a poisonous substances classification, an official classification provided by a para-governmental organisation which includes toxicologists. This legislation may refer to other texts: categories of danger for transport (the Eu...
M Romeis, P Guéneau
Safe handling of solvents in relation to classification and labelling
1990 - IRG/WP 3569
The classification and labelling of chemical products, safety data sheets and personal protective equipment are topics which at present are given a very high priority in Denmark. The area is very large and may seem extremely difficult to comprehend. The frequent publication of acts and announcements from the authorities does not make the work any easier. In the following I will try explain how we ...
J Haugaard
What is OECD doing to promote harmonization of biocide regulations
1999 - IRG/WP 99-20182
OECD began work on biocides in 1997 to help Member countries co-operate in the assessment and registration of these products. The work is a part of OECD's Pesticide Programme, and is co-ordinated by the Biocides Steering Group. The goal is to harmonize regulatory approaches to allow countries to conduct evaluations of biocides more efficiently. More efficient evaluations can advance the p...
M Paneli, N Grandy