Your search resulted in 9 documents.
Characterization of tropical wood-decaying fungi by RFLP analysis of PCR amplified rDNA
1998 - IRG/WP 98-10251
Five brown-rot fungi (Tyromyces palustris, Gloeophyllum trabeum, Antrodia sp., Poria placenta, Coniophora puteana) and three white-rot fungi (Coriolus versicolor, Pycnoporus sanguineus, Lentinus squarrosulus) registred as representative wood-decaying fungi for international standards of wood durability tests have been characterized using molecular tools (PCR/RFLP). The Internal Transcribed Spacer ...
A Zaremski, M Ducousso, Y Prin, D Fouquet
Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing
2004 - IRG/WP 04-10512
Fungi selected in test fields in Germany and Estonia and fungi (Coniophora puteana) cultivated on medium have been identified by the molecular methods PCR (Polymerase Chain Reaction), T-RFLP (Terminal Restricted Fragment Length Polymorphism) and sequenced to species level. The samples from the German field test showed that the fruit body sample was a different fungus than from the mycelium sample....
U Råberg, N Högberg, C J Land
Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt
A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl
Identification of fungi colonising coated and modified wood exposed outdoors using sequencing and T-RFLP profiling
2006 - IRG/WP 06-20326
Wood decay and staining fungi are known to colonize coated and modified wood. Rapid and reliable ways of identifying fungi on coated and modified wood may improve diagnostics and product development. In this work nine fungi growing in painted wood panels exposed outdoors at a test field in Uppsala, Sweden, were identified using sequencing and T-RFLP (Terminal Restriction Fragment Length Polymorphi...
U Råberg, J Bijelovic, C J Land, S Bardage, N Terziev
Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP I: Accelerated laboratory decay study
2006 - IRG/WP 06-20332
The effects of Chlorothalonil (CTN) and Butylated Hydroxy Toluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and BHT is being evaluated for its synergistic effects with CTN. Results from an accelerated decay test will be presented. The...
G T Kirker, M L Prewitt, S V Diehl
Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP II: Results from field studies
2007 - IRG/WP 07-30429
The effects of Chlorothalonil (CTN) and Butylated Hydroxytoluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and the non-biocidal BHT was evaluated for its synergistic effects with CTN. ACQ-C was a positive control and untreated SYP sta...
G T Kirker, M L Prewitt, S V Diehl
Microbial Community Analysis Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis: Field Study Results
2008 - IRG/WP 08-20377
The effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on the fungal community in southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis. Field stakes treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1 and 0.25% CTN, and 0.1 and 0.25% CTN combined with 2% BHT were installed with untreated controls in field s...
G T Kirker, S V Diehl, M L Prewitt, W J Diehl
Microbial Community Analysis of Naturally Durable Wood in an Above Ground Field Test
2014 - IRG/WP 14-10826
This paper presents preliminary results of an above ground field test wherein eight naturally durable wood species were exposed concurrently at two sites in North America. Surface samples were taken at regular intervals from non-durable controls and compared to their more durable counterparts. Terminal Restriction Fragment Length Polymorphism was performed to characterize the microbial (bacteria, ...
G T Kirker, S V Diehl, P K Lebow