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The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
The activities of one-electron oxidation, hydroxyl radical generation, and phenol oxidase during the incubation of cultures of four white-rot fungi containing either glucose or wood were periodically measured. Further, their degradation activities against wood were examined during the course of cultivation. The generation of hydroxyl radical was correlated to the activity of wood degradation and i...
H Tanaka, S Itakura, A Enoki


Preliminary observations on tributyltin treated Scots pine sapwood using 119Sn (Mössbauer and NMR) spectroscopic techniques
1984 - IRG/WP 3312
119mSn Mössbauer data are presented for bis(tributyltin) oxide, tributyltin ethanesulphonate, bis(tributyltin) carbonate and tris(tributyltin) phosphate, both in the pure form and in Scots pine sapwood. The structures proposed for these compounds, in the solid state, are supported by 119Sn nuclear magnetic resonance spectroscopy.
R Hill


Northern oak wood and its damages in Iran
1988 - IRG/WP 1377
The Northern oak species of Iran designated Quercus castaneaefolia C.A.M. is distributed widely in caspian littoral. In natural conditions (healthy) has favourable quality and it is used mostly in rural construtions, sleepers, parquets, cross arms for electric and communication poles, it is used also indoor and window construction, benches and finally veneer. This wood in the case of sleepers and ...
D Parsapajouh, P Niloufari


Influence of the decay of spruce chips by the selected fungi on their chemical structure and the pulp properties
2004 - IRG/WP 04-10510
The paper focuses on changes in the weight and in the molecular structure of spruce chips submitted to long-term storing as well as to medium- and long-term model rotting degradation caused by some chosen fungi identified at storing processes on the pile. For the model decay of chips under laboratory conditions during 3 or 6 weeks the white-rot fungi: Phanerochaete chrysosporium, Heterobasidion an...
R Solár, L Reinprecht, A Geffert, F Kacík


Decomposition of biocides in aqueous media cases of MTC and TCMTB
1993 - IRG/WP 93-50001-26
Our laboratory is interested in the fate of modern biocide molecules in the environment. Here we present recent work which has been done on the fate of two compounds with fungicidal and bactericidal properties: methylenedithiocyanate (MTC: NCS - CH2 - SCN) and thiocyanomethylthiobenzothiazol (TCMTB). We have studied separately the influence of pH, temperature and ultraviolet irradiation in aqueous...
R Perraud, M Papazian


Long-term preservation technology for a large-scale timber structure building in Japan
2001 - IRG/WP 01-40203
Several 1.000-year-old Buddhist temples and shrines built of wood are surviving in Nara, some areas of which are inscribed on the World Heritage List. These old buildings are indicative of excellent timber construction technology having existed in ancient Japan, though it is thought that such technology has not been handed down. This paper introduces one of large-scale wood buildings built in rece...
Y Iimura


Subterranean termites of economic importance in suburban Melbourne: Wood structures most frequently attacked and damaged
1990 - IRG/WP 1430
An examination of wood structures in forty buildings actively being attacked by subterranean termites in Melbourne revealed that all buildings were being attacked and damaged by Coptotermes species. This indicates the economic importance of these species. Hardwoods were more frequently infested than softwood species. The implications of these findings are discussed in relation to termite control, ...
J R J French


The action of siderophores isolated from Gloeophyllum trabeum on the structure and crystallinity of cellulose compounds
1991 - IRG/WP 1479
Low molecular weight, high affinity iron-binding compounds (siderophores) were isolated from the brown-rot fungus Gloeophyllum trabeum. The compounds were shown to be inducible by iron starvation and could be purified by ultra-filtration, ethyl acetate extraction, column chromatography and preparative HPLC. The isolated compounds were shown by analytical and immunological techniques to be produced...
J Jellison, V Chandhoke, B Goodell, F Fekete, N Hayashi, M Ishihara, K Yamamoto


A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl


Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA). The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth


Distribution and genetic variation of Reticulitermes (Isoptera: Rhinotermitidae) in Portugal
2006 - IRG/WP 06-10581
The genus Reticulitermes has a Holartic distribution and is widely represented in southern Europe. It is established in Portugal, across the full range of ambient environmental conditions, using different types of wood resources, as available, and nesting ubiquitously in many types of soil. Sequencing of part of the mtDNA COII gene revealed that all the specimens sampled on a N-S transect of the c...
T Nobre, L Nunes, P Eggleton, D E Bignell


Molecular characterization of M’jej, decaying agent of cedar forests in Morocco
2006 - IRG/WP 06-10593
Cedar wood is well appreciated since thousand years even though its economical importance is limited because of its world distribution restricted to some Mediterranean countries and Himalaya. The most important species, Cedrus atlantica have its biggest population in Morocco (130 000 ha) where it is submitted to fungal diseases. Among them, the locally named “M’jej” reduced notably saw mile ...
A Zaremski, S Bakkali-Yakhlef, C Chaintreuil, Y Abbas, Y Prin, M Abourouh, M Ducousso


Detecting fungal DNA in treated and non-treated wood
2007 - IRG/WP 07-10621
Isolating fungi from wood has long involved culturing on selective media followed by identification using various keys. This process can be cumbersome, costly, and, most importantly, not always capable of detecting all of the fungi present. The recent development of molecular methods for isolation and identification of fungi has created tremendous opportunities for expanding our knowledge of the...
C Freitag, M Freitag, J Morrell


Molecular methods as a tool within the field of wood protection – available methods and new possibilities
2007 - IRG/WP 07-20361
The area of wood protection is in a period of change. New tools are needed to understand the mode of action, and to further improve the new wood protection systems. A set of useful tools are found among the molecular methods. This paper presents an overview of some of the tools available, and the methods are exemplified by papers within the frame of wood protection issues. However, there is stil...
G Alfredsen, A M Hietala, C G Fossdal, H Solheim


A Review of Wood as Material and Source of Innumerable Products
2008 - IRG/WP 08-10662
As we known that “wood –which is a source of innumerable pruducts (Tsoumis, 1968)– is a fascinating and challenging material (Dinwoodie, 1975)”, and hence, “an understanding of the basic properties of wood and of the factors that affect its serviceability is vital background for the preservation of wood structures (Sherwood, 1986)”. This paper therefore presents an overview of structru...
I Usta, R Despot


The uses of Molecular techniques in studying Australian subterranean termites Genus Coptotermes
2008 - IRG/WP 08-10669
This study focuses on applying molecular tools in studying the phylogeny of the Coptotermes, especially from the Australian region, by using a combined data set of three mitochondrial genes, viz. 16S, COII, COI, comprising a total of about 2000 base pairs. This study will address these following specific questions: i. Does molecular phylogenetics reveal any new species and show any previously ...
H M Badawi, B M Ahmed (Shiday), J R J French, M P Schwarz


Antifungal activity of different molecular weights of a biopolymer chitosan against wood decay fungi
2008 - IRG/WP 08-30456
In recent years chitosan has been investigated as a natural polymer for wood preservation against fungal decay. From an environmental point of view, chitosan seems to possess a potential approaches as wood protecting agent. In this study, three different molecular weights of chitosan compounds (with approximately the same degree of deacetylation 83 ± 2 %) were evaluated as in vitro and in vivo as...
A S O Mohareb, M E I Badawy


Molecular Methods: a Reliable Tool for the Identification of Wood Decay Fungi in Construction Timber
2008 - IRG/WP 08-20386
In the present study, we tested the practical value of several DNA-based methods to identify at the species level the most common wood-decaying fungi infecting buildings. We successfully extracted and amplified fungal DNA from pure cultures of twelve species of wood-inhabiting fungi, from oak and pine wood infected in laboratory with known strains, and from unknown field samples of wood collected ...
M Maître, M Kutnik, I Le Bayon, L Harvengt


Formation and Structure of Metal Azole Complexes
2008 - IRG/WP 08-30469
Divalent copper and zinc complexes with metal:azole ratio 1:2 were readily formed at room temperature with the fungicides tebuconazole and propiconazole. The structure of copper and zinc tebuconazole acetate and zinc cis-propiconazole chloride were examined by X-ray crystallography. In copper tebuconazole acetate, the copper atom lies on a crystallographic inversion centre and is coordinated to tw...
P D Evans, K J Schmalzl, C M Forsyth, G D Fallon, S Schmid, B Bendixen, S Heimdal


Radial flow of Bornmullerian fir (Abies bornmulleriana Mattf.) as affected by wood tapering and the condition of end wall structure of uniseriate ray parenchyma cells
2008 - IRG/WP 08-40441
Amenability to radial permeability of Bornmullerian fir (Abies bornmulleriana Mattf.) was studied on the base of the effects of wood tapering and the structure of end walls of uniseriate ray parenchyma cells. The results showed that the most remarkable culprits of the greatest fluid uptake (as the percentage of void volume filled by the fluid in the radial flow direction, RVVF%) are the lesser woo...
I Usta, S Aslan


Wood Decay Research Using Molecular Procedures, What Can It Tell Us?
2008 - IRG/WP 08-10678
There are many unanswered questions in wood decay and protection research, and no single research technique will ever answer all of these questions. The process of wood decay is a very complex series of biochemical and chemical reactions that are heavily influenced by the hundreds of bacterial and fungal species found on environmental samples of wood. There are a variety of molecular techniques b...
S V Diehl, M. L Prewitt, Young-Min Kang, L Mangum, J D Tang


Degradation of wood veneers by Fenton’s reagents: effects of low molecular weight phenolic compounds on hydrogen peroxide decay and tensile strength loss
2009 - IRG/WP 09-20400
Pine wood (Pinus sylvestris) veneers strips were incubated in acetate buffer containing hydrogen peroxide and iron to mimic mechanisms of brown rot decay and assess the degradation of cellulose through analysis of the tensile properties of the decayed wood. The tensile properties of thin wood strips treated with Fenton system reagents or precursors were determined and correlated to weight loss as ...
Yanjun Xie, R Well, Zefang Xiao, B Goodell, J Jellison, H Militz, C Mai


Effect of Preservative Treatment on Fungal Colonization of Teak, Redwood, and Western Red Cedar
2009 - IRG/WP 09-20404
Fungal flora present in preservative treated samples or non-treated samples from sapwood and heartwood of teak, western red cedar, redwood, and southern yellow pine was assessed after 6 to 18 months of exposure near Hilo, Hawaii. The objectives were to compare fungal composition and diversity between treated and non-treated samples, and to examine the use of molecular techniques for assessing fung...
Y Cabrera, C Freitag, J J Morrell


Evaluation of exposure conditions for wooden facades and decking
2009 - IRG/WP 09-20408
During their functional life, building components are exposed to moisture and other environment stress conditions in numerous ways. This is a fact that should be taken into account during manufacture, product development, designing and work execution of building components. For wood material, moisture stress and biological factors like mould and decay fungi are often critical in cladding and decki...
H Viitanen, T Toratti, R Peuhkuri, T Ojanen, L Makkonen


Fungal Attack on Lignin and Cellulose: Elucidation of Brown- and White-Rot Mechanisms Comparing Biomimetic and In-Vivo Degradation Patterns
2010 - IRG/WP 10-10714
This paper examines research and hypotheses that have been developed over several years on wood degradation mechanisms. This information is combined with new data and analyses to explain why wood decay patterns caused by brown-rot fungi and specific types of white-rot fungi are different. New data, including work with both biomimetic studies on low molecular weight compounds, degradative enzymes, ...
V Arantes, B Goodell, A M F Milagres, Yuhui Qian, T Filley, J Jellison, S Kelley


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