IRG Documents Database and Compendium


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Identification card for Coriolopsis polyzona (Pers.) Ryv
1977 - IRG/WP 161
A Ofosu-Asiedu


Isolation and identification of non-decay fungi affecting the performance of alkylammonium compounds
1993 - IRG/WP 93-10020
The isolation of DDAC tolerant fungi was carried out on lumber treated with a range of antisapstain chemicals containing DDAC as one of their active ingredients. The tolerant fungi were selected by using malt media spiked with DDAC (100 and 2500 ppm). Isolations were tentatively identified into a range of fungi commonly found associated with wood deterioration (i.e. Penicillium sp. and Trichoderma...
A K Doyle, J N R Ruddick


Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt


A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl


Identification and inhibitory effect of volatiles from different ages of a Trichoderma aureoviride culture on selected wood decay fungi.
1995 - IRG/WP 95-10110
The ability of a Trichoderma sp. to produce volatile organic compounds (VOCs) over a four week period of growth was examined and the inhibitory effect of these volatiles against four selected basidiomycetes over the same period was assessed. After trapping, on tubes filled with chromatography packing material, VOCs were analysed on an integrated automated thermal desorbtion mass spectrometer syste...
A Bruce, A Kundzewicz, R E Wheatley


Wood decay fungi from New Zealand ‘leaky’ buildings: PCR identification and laboratory decay tests of wood preservative-treated Pinus radiata (Part 1)
2007 - IRG/WP 07-10620
Fungi colonising Pinus radiata D. Don framing timber of ‘leaky’ New Zealand buildings were isolated to produce pure cultures. Mycelia from these cultures on agar media were collected to extract DNA. To identify the fungi to the species level, polymerase chain reaction (PCR) with primer pairs ITS1-F and ITS4 were performed followed by sequencing of the internal transcribed spacer (ITS) region. ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Wood decay fungi from New Zealand leaky buildings – PCR identification (Part 2) and aerial spore trapping
2008 - IRG/WP 08-10649
Prior to this study, it was not know which species of decay fungi caused decay in New Zealand leaky buildings. Use of molecular biology methodology, polymerase chain reaction (PCR), and subsequent DNA sequencing, as well as classical mycological techniques based on morphology, has enabled identification of decay fungi and has provided insight into their relative importance based on isolation frequ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Molecular Methods: a Reliable Tool for the Identification of Wood Decay Fungi in Construction Timber
2008 - IRG/WP 08-20386
In the present study, we tested the practical value of several DNA-based methods to identify at the species level the most common wood-decaying fungi infecting buildings. We successfully extracted and amplified fungal DNA from pure cultures of twelve species of wood-inhabiting fungi, from oak and pine wood infected in laboratory with known strains, and from unknown field samples of wood collected ...
M Maître, M Kutnik, I Le Bayon, L Harvengt


Aislamiento, identificación y evaluación enzimática de hongos de pudrición de madera de la Región de los Lagos
2008 - IRG/WP 08-10680
So far, systematic investigations have not been developed to determine the fungal diversity associated with wood in Chile. In addition, little is known about the mycoflora capabilities or their enzymatic processes in biotechnology although different research has hinted at the great potential of these microorganisms in various industrial processes. The aim of this work is to isolate, identify and e...
R Ortiz, J Navarrete, C Oviedo, R Blanchette


Assessment of decay risk of airborne wood-decay fungi
2012 - IRG/WP 12-10787
The decay risk of airborne wood-decay fungi was investigated by using an air sampler. Japanese cedar disks measuring about 8 cm in diameter and 3 mm in thickness with moisture content at about 100 % were placed in a “BIOSAMP” air sampler and exposed to 1000 liters of air. Air sampling was carried out from June to September at the same sampling site in Tsukuba, Japan. The exposed disks were the...
I Momohara, Y Ota, K Sotome, T Nishimura


What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
Molecular techniques are now routinely used in the identification, detection and analysis of wood degrading organisms. An overview of some of the early work on nucleic acid isolation and characterization will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of pre...
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace


Identification of Antifungal Compounds in Konjac Flying Powder and Assessment against Wood Decay Fungi
2019 - IRG/WP 19-30737
The antifungal activity of konjac (Amorphophallus rivieri) flying powder (a by-product produced during mechanical processing of konjac flour) ethanol extract was evaluated against wood decay fungi in culture. Compounds associated with antifungal activity in the extracts were isolated and purified by silica gel column chromatography. The antifungal active fractions were identified by ultra-high-per...
Z Bi, F Yang, Y Lei, J J Morrell, L Yan


Effect of acetylation on decay resistance of wood against brown-rot, white-rot and soft-rot fungi
1989 - IRG/WP 3540
Effect of acetylation on decay resistance of wood was investigated using wood blocks of Cryptomeria japonica, Pinus densiflora, Albizia falcata and Fagus crenata. Blocks were treated with uncatalyzed acetic anhydride for different lengths of time and exposed to Tyromyces palustris, Serpula lacrymans, Coriolus versicolor and unsterilized soil. The action of OH-radical on acetylated wood was also ex...
M Takahashi, Y Imamura, M Tanahashi


Questionnaire - Fungal decay types
1985 - IRG/WP 1265
T Nilsson


JWPA method for testing effectiveness of surface coatings with preservatives against decay fungi
1981 - IRG/WP 2164
In 1979 JWPA established a new method for testing effectiveness of surface coatings in accordance with practical use of preservative-treated lumber. Comparing the new testing method with JIS A 9302, a few new trials - size of wood specimen, weathering procedure, and decay-test procedure - are incorporated....
K Tsunoda


Nondestructive Evaluation of Oriented Strand Board Exposed to Decay Fungi
2002 - IRG/WP 02-20243
Stress wave nondestructive evaluation (NDE) technologies are being used in our laboratory to evaluate the performance properties of engineered wood. These techniques have proven useful in the inspection of timber structures to locate internal voids and decayed or deteriorated areas in large timbers. But no information exists concerning NDE and important properties of wood composites exposed to dec...
B Illman, V W Yang, R J Ross, W J Nelson


Moisture content levels and decay of hemlock
1986 - IRG/WP 1287
As a model of decay conditions of wooden members in wooden houses, a decay test was set up in which samples of western hemlock (Tsuga heterophylla) under 4 moisture levels were examined. Each week the samples were weighed and if the weights indicated that their moisture contents were lower than the expected levels, distilled water was added. Every 8 weeks 3 samples from each condition were oven dr...
K Suzuki


Monographic cards for wood-destroying fungi. [Fiches monographiques pour les champignons lignivores]
1970 - IRG/WP I 5B
C Jacquiot


On Donkioporia expansa (Desm.) Kotl. & Pouzar
1986 - IRG/WP 1285
Donkioporia expansa is found more often in houses than realised until now. Virulence tests according to EN 113 show not only an attack of oak, but also of other hardwoods and even soft-woods....
G Buchwald


Additions and corrections to recent names for some common decay fungi
1977 - IRG/WP 167
A Käärik


Effects of acetylation on the dimensional stability and decay resistance of kenaf (Hibiscus cannabinus L.) fiberboard
1996 - IRG/WP 96-40059
The objective of this study was to investigate the influence of the acetylation treated kenaf fiber, Phenol formaldehyde resin content level, and three fungi species on the dimensional stability and decay resistance of high density non wood composition boards. A standard ASTM method was used to evaluate weight loss and thickness change. The linear shrinkage and expansion of each species were also ...
P Chow, T Harp, R Meimban, J A Youngquist, R M Rowell


The dry rot fungus and other fungi in houses. Part 2
1993 - IRG/WP 93-10001
J Bech-Andersen


Valid names for some common decay fungi, their synonyms and vernacular names
1978 - IRG/WP 172
A Käärik


Physical properties of ß-1,4-Xylanase produced by Postia (=Poria) placenta: Implications for the control of brown rot
1987 - IRG/WP 1318
The degradation of hemicelluloses is an early event in wood decay by brown-rot fungi. An understanding of the physical properties of hemicellulases may suggest target mechanisms for the development of new control agents. Endo-b-1,4-xylanase was partially purified by column chromatography from wood decayed by Postia (= Poria) placenta. The enzyme was extremely resistant to denaturing conditions; no...
J A Micales, F Green III, C A Clausen, T L Highley


A laboratory method for assessing the effectiveness of fungicides in preventing the spread of decay fungi within packages of unseasoned lumber
1983 - IRG/WP 2202
To study the deterioration caused by decay fungi in the laboratory, a method for testing fungicides for their effectiveness in preventing spread of decay was devised. Some experiments using this method are reported here....
A J Cserjesi, E L Johnson, A Byrne


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