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Ingestion of wood-degrading micro-organisms
1991 - IRG/WP 4169
An ultrastructural study was carried out on the digestive tract contents of Limnoria lignorum, frass, gallery walls and surface structures of the animals. The purpose of the study was to determine whether wood-degrading bacteria, fungi and other microbes and/or wood degraded by these microbes were present in gut regions and therefore could provide indirectly a nutritional source for Limnoria. Both...
G F Daniel, S M Cragg, T Nilsson


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson


Extracellular osmiophilic particles in connection with brown rot and white rot
1982 - IRG/WP 1157
The way of attack on Pine woodblocks of two brown-rot fungi (Fomitopsis pinicola, Coniophora puteana) differing in their cellulase activities, and one white-rot fungus (Trametes hirsuta) has been investigated by transmission electron microscopy. Even at early stages of decay, osmiophilic particles were found with all the fungi investigated. In the brown-rotted wood, the osmiophilic particles occur...
K Messner, H Stachelberger


Cavitation bacteria
1984 - IRG/WP 1235
A form of bacterial wood degradation is described. The term "cavitation bacteria" has been coined in order to describe the discrete cavities that are formed within the wood cell walls. The bacteria observed within the cavities appeared to be polymorphic with rounded as well as filamentous forms recognised. TEM studies suggest that the bacteria produce diffusable wood-degrading enzymes. Cavitation ...
T Nilsson, A P Singh


Bending strength of heat-treated spruce and pine timber
2002 - IRG/WP 02-40242
Heat-treatment of spruce (Picea abies) and pine (Pinus sylvestris) beams (45 x 145 mm) gave a reduction in bending strength of approximately 50%. The bending stiffness (modulus of elasticity) only decreased by 3.5%. Tests were carried out according to EN 408. Another effect of the heat-treatment was increased variation in bending strength. The 5th percentile value of the bending strength, the so c...
C Bengtsson, J Jermer, F Brem


The development of soft rot decay in bamboo fibres
1992 - IRG/WP 92-1572
The development of decay by soft rot fungus Chaetomium globosum was investigated in young and mature culms of bamboo Phyllostachys virideglaucescens. Soft rot decay was influenced both quantitatively and qualitatively by the differing levels of lignification in the material. Weight loss was significantly greater in the younger culm compared with the older, more heavily lignified culm. The distribu...
O Sulaiman, R J Murphy


Bacterial degradation of wood cell wall: A review of degradation patterns
1990 - IRG/WP 1460
Information from bacterial degradation studies of 60's and 70's was reviewed by Nilsson in 1982. The application of electron microscopy to this area in recent years has provided much useful information and has eliminated earlier scepticism among workers about the ability of bacteria to degrade lignified wood cell walls. Studies using transmission electron microscopy together with...
A P Singh, J A Butcher


Preliminary pole treatment trials with chlorothalonil in hydrocarbon solvent
1994 - IRG/WP 94-40027
A series of 460-mm long, end-sealed southern pine pole stubs were treated with chlorothalonil in hydrocarbon solvent. Over sixty sections were treated using various combinations of initial seasoning (kiln-drying, steam-conditioning), treating temperature (ambient, 200°F), initial air pressure, and final conditioning (steam flash + vacuum, expansion bath + vacuum, steam distillation + vacuum). Dis...
H M Barnes


Utilisation of carbohydrates by stain fungi in agar culture
1998 - IRG/WP 98-10248
Stain fungi are often defined by their ability to utilise the starch and free sugars found in ray parenchyma cells, and their inability to utilise other wood constituents. However, several species of stain fungi produce bore holes in wood cell walls. This suggests that enzymatic activity capable of degrading structural polysaccharides and/or lignin is associated with the growth of the appressorium...
J Snow, P Vinden, S M Read


Degradation of woods by obligately anaerobic fungi
1990 - IRG/WP 1452
Little is known about the processes occurring during the degradation of wood by obligately anaerobic fungi. When woods from a range of hardwood species were incubated for 11 days with a strain of the Chytrid Neocallimastix frontalis many of the woods were measurably fermented; Fagus sylvatica (23%) was the most highly degraded. Microscopic examination of Populus tremuloides wood after incubation w...
K N Joblin, G E Naylor


A Novel Type of Multiple Cavity Attack in Wood Cell Walls of Heat-treated Timber Exposed in Seawater – Preliminary Observations
2004 - IRG/WP 04-10523
Samples of untreated and heat-treated Norway spruce and Douglas fir were submerged in Langstone Harbour, Portsmouth for 4 years at a depth of ca. 0.3 metres. The heat-treated samples had been prepared using the Plato process. Samples were initially assessed for the severity of marine borer damage and were then examined microscopically for evidence of microbial decay. Longitudinal and transverse wo...
R A Eaton, C Björdal, T Nilsson


Exclusion of anions from the wood cell wall
1991 - IRG/WP 3661
Anionic components of wood preservatives and fire retardants such, as arsenates and phosphates, equilibrate at much lower concentrations in the cell wall bound water than in the free solution in the cell lumens. A degree of anion exclusion is expected in wood, due to the Donnan membrane effect. Fixed anions in the wood cell wall, produced by pH-dependent dissociation of the weak acid groups in woo...
P A Cooper


Chitin synthetase activity in cellular fractions of wood-decay fungi
1992 - IRG/WP 92-1524
This study was an attempt to explain the variability of in vivo sensitivity of wood decay fungi to the chitin synthetase (CS) inhibitor, polyoxin, by comparing CS distribution in cellular fractions. The five species studied were the brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, Fibuloporia vaillantii, and the white-rot fungi Schizophyllum commune and Ganoderma applanatum. The CS activi...
B R Johnson, S C Croan, B Illman


The potential of high pressure pulsation processes to treat white spruce lumber with water-borne preservatives
1988 - IRG/WP 3471
Laboratory work using end sealed 4x8x46 cm³ white spruce samples has been done to explore the suitability of three variants of a 2.1 MPa pulsation process for the impregnation of white spruce with CCA. The results showed that the process improved significantly the penetration of the preservatives and reduced significantly cell collapse, when compared with the results of treatment using a 2.1 MPa ...
J P Hösli, J N R Ruddick


Amenability of radial permeability of Sitka spruce (Picea sitchensis (Bong.) Carr.) as affected by aspects of cell ends in uniseriate ray parenchyma tissue
2003 - IRG/WP 03-40252
The principal objective of this article was to recognise and understand the amenability of ray parenchyma cell ends (end platform) in uniseriate ray tissue to influence the radial permeability of Sitka spruce (Picea sitchensis (Bong.) Carr.) by examination of most (Queen Charlotte Islands in Rhondda, South Wales) and the least (South Oregon in Dalby, North-East England) seed origins that grown in ...
I Usta, M D C Hale


Multiple-Phase Pressure (MPP) Process: One-stage CCA treatment and accelerated fixation process. 3. Effect of process variables on sapwood treatment and CCA fixation.
1998 - IRG/WP 98-40114
The Multiple-Phase Pressure (MPP) Process achieves treatment and fixation as combined process using hot CCA, within a 2-3 hour time-frame. Timber is treated to saturation with hot CCA using an empty cell process. During the pneumatic pressure phase, hot solution is held in the wood in an otherwise liquid-free treatment vessel until fixation is >95% complete. This report details a factorial des...
H Pearson, K Nasheri, J A Drysdale, G Durbin, M E Hedley


Time-of-Flight Secondary Ion Mass Spectrometry Analysis of Polyethylene Glycol Penetration into Wood Cell Walls
2006 - IRG/WP 06-20328
Penetration of high molecular weight polyethylene glycol (PEG) into wood cell walls was investigated in red pine. PEG of different molecular weights (1000, 8000 and 20000) was labelled by bromination and treated samples were analyzed on Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) in dynamic mode. Both negative and positive spectra of the samples were collected in high spatial (bunche...
D Jeremic, P Cooper, P Brodersen


Micro-Distribution of Metals in Wood Treated with a Nano-Copper Wood Preservative
2007 - IRG/WP 07-40360
The microdistribution of copper in southern pine treated with a newly-developed nano-copper wood preservative was examined to determine if it differed from that reported for wood treated with conventional copper-based wood preservatives. Field Emission Scanning Electron Microscopy (FE-SEM) in combination with x-ray microanalysis (EDX) revealed the presence of nano-sized copper and iron particles i...
H Matsunaga, M Kiguchi, P Evans


Fire Safety of Wood Floor Assembly: Model and Full-scale Test
2007 - IRG/WP 07-20375
The present paper describes the model for the prediction of fire safety of wood floor assemblies. The model includes heat transfer model for the calculation of the flow of heat in floor assembly and structural model for the analysis of the mechanical performance of wood joists. The floor assemblies considered in this paper are constructed with nominal 2x10 (38x241mm) wood joists lined by Type X ...
H Takeda


Micro-Distribution of Micronized Copper in Southern Pine
2008 - IRG/WP 08-30479
For copper-based preservatives to be used in ground contact, penetration of copper into the cell wall is believed to be important to protect the wood from soft rot fungi. Preservatives containing soluble copper are known to do this. It is not known whether preservatives containing particulate copper will also migrate into the cell wall in sufficient quantities to control soft rot decay. An AWPA st...
R Stirling, J Drummond, Jun Zhang, R J Ziobro


SEM-EDXA of CCA-treated Rubberwood (Hevea brasiliensis)
2009 - IRG/WP 09-20399
The microdistribution of chromated copper arsenate (CCA) preservative in rubberwood was studied using scanning electron microscope in conjunction with energy dispersive X-ray analyzer (SEM-EDXA). Bulk X-ray analysis showed that there was a high accumulation of chromium, copper, and arsenic in the vessels and lower concentration of the three preservative elements in fibres. Chromium appeared to rec...
I Jusoh, D P Kamdem


Re-Distribution of Copper in the Cell Walls of Wood Treated with Micronized Copper Quat
2009 - IRG/WP 09-30506
Wood treated with copper-based preservatives to be used in ground contact may fail to inhibit soft rot fungi if penetration of copper into the cell wall is insufficient. Preservatives containing soluble copper are known to penetrate the cell wall; however, it is not known whether preservatives containing particulate copper will also migrate into the cell wall in sufficient quantities to control so...
R Stirling, J Drummond


Leaching of the copper component from full scale decking boards during one summer season
2009 - IRG/WP 09-50260
The leaching of copper has been tested in laboratory and in outside exposure for freshly treated pine sapwood samples with three different copper preservatives, Cu HDO, copper quat and copper triazoles. We found in the laboratory leaching test that a fixation with warming to 60 °C (140 °F) for 48 hours without drying and then drying in the laboratory in room temperature gave the lowest leaching of copper. We also found that surface treatments with wood oils reduced the leaching, and that washing of the surface before testing had no effect. Full scale decking board samples (0.25 m2) were then exposed outdoors for rain. The rain water was collected and analysed for copper. After one summer season (about 600 mm rain) we found that the leaching differ for the three preservatives. All samples with a water borne surface treatment had the lowest leaching, about half the amount of untreated.
F G Evans


Protection mechanisms of modified wood against decay by white and brown rot fungi
2010 - IRG/WP 10-10713
The resistance of beech and pine wood blocks treated with 1,3-dimethylol-4,5-dihydroxyethylene urea (DMDHEU) against T. versicolor and C. puteana increased with increasing WPG. Full protection (mass loss below 3%) was reached at WPGs of approximately 15% (beech) and 10% (pine). Metabolic activity of the fungi in the wood blocks was assessed as heat or energy production determined by isothermal mic...
C Mai, P Verma, Yanjun Xie, J Dyckmans, H Militz


Copper nanoparticles in southern pine wood treated with a micronised preservative: Can nanoparticles penetrate the cell walls of tracheids and ray parenchyma?
2010 - IRG/WP 10-30547
This study tests the hypothesis that copper nanoparticles can penetrate the cell walls of southern pine wood treated with a micronised preservative. We examined the nanodistribution of particles in tracheid and ray parenchyma cell walls using state-of-the-art HR (High Resolution)-TEM and HR-STEM (Scanning Transmission Electron Microscope)-EDX. These devices are capable of atomic-scale resolution. ...
H Matsunaga, Y Kataoka, M Kiguchi, P Evans


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