Your search resulted in 19 documents.
Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
The manner by which lignin is degraded in-situ in natural substrates by white rot fungi still remains a controversial issue particularly the distribution and role(s) played by lignin degrading enzymes (i.e. manganese II peroxidase and lignin peroxidase). In the present study, use was made of anti-manganese II peroxidase and immunolabelling techniques in conjunction with transmission electron micro...
G F Daniel, B Pettersson, T Nilsson, J Volc
An alternative method for the determination of lignocellulolytic enzymatic activities of white rot fungi
1998 - IRG/WP 98-10261
Some lignocellulolytic activities of wood decay fungi can be evaluated in an indirect way through the study of the decolorization that white rot fungi produces in culture media in the presence of dyes.
In this assay, ten dyes were selected, which were added in concentrations of 0.00 1% - 0.003% (P/V) to four-day cultures of Trametes versicolor containing 1% of beech sawdust in a basal salt medium...
D Muñoz-Mingarro, F Llinares, F Rubio, M Yuste, C Rodríguez-Borrajo, J Jiménez, M T De Troya
Enzymatic study of Ceratocystis sp., blue-stain fungi on Pinus nigra
1999 - IRG/WP 99-10315
One of the main problems that the forest exploitation industry has with Pinus nigra wood is the blue-stain fungi, whose causing agent is unknown. Therefore, the objective of this work has been to study, through enzymatic tests of the isolated cultures, if these fungi infect Pinus nigra in any specific way. After the incubations, isolates of Ceratocystis were obtained. These were cultured in a sali...
M T De Troya, F Rubio, D Muñoz-Mingarro, F Llinares, C Rodríguez-Borrajo, M Yuste, M J Pozuelo, J I Fernández-Golfín
Free radical process controlled by manganese peroxidase and lipid-related metabolites produced by Ceriporiopsis subvermispora
2001 - IRG/WP 01-10412
Ceriporiopsis subvermispora, a specific lignin-degrading fungus produced free unsaturated fatty acids (USFAs) including 9,12-octadecaienoic asid, together with saturated fatty acids (SFAs) at an incipient stage of cultivation on wood meal cultures. In prolonged cultivation period after two weeks, the amount of intact fatty acids decreased with increasing in organic hydroperoxide and TBARS producti...
T Watanabe, M Enoki, S Sato, Y Honda, M Kuwahara, N Shirai, K Messner
Degradation of polychlorinated organic biocides by the wood decaying fungi
2008 - IRG/WP 08-50253
Organochlorine biocides such as lindane, pentachlorophenol sodium salt, and polychlorinated biphenyl 153 have been widely used as wood preservatives. Due to their low degradability by soil bacteria, treated waste wood products pose a serious threat to the environment. Consequently, there is an increased need for development of new strategies for their detoxification. In this work, the bioremediati...
I Vidic, L Zupancic-Kralj, K Sepcic, F Pohleven
Effect of Heavy Metals on the Expression of Manganese Dependent Peroxidases of Phanerochaete chrysosporium
2010 - IRG/WP 10-10722
This work is part of an ongoing investigation that studies the application of a solution rich in Zn and Mn, obtained from a recycling process, as a preservative for wood. The effects of Zn2+ and Mn2+ on the enzyme-expression level of manganese-dependent peroxidase (MnP) from Phanerochaete chrysosporium was studied by real-time PCR. The glyceraldehyde 3-phosphate dehydrogenase gene (gpd) was used a...
C Ibáñez, M Rabinovich, M Barraco, G Gecchetto, M Cerdeiras
Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola
1988 - IRG/WP 1343
By applying immunoelectronmicroscopic methods, lignin peroxidase of the white rot fungus Phanerochaete chrysosporium has been localized in the cytoplasm of hyphae, close to the plasmalemma and on the plasmalemma. Infiltration of wood specimen with culture filtrates, concentrated 300-fold, gave clear information on the penetration of the enzyme into the wood cell wall. Penetration was restricted to...
E Srebotnik, K Messner
Occurrence of manganese deposits in test stakes exposed in groung contact situations
1996 - IRG/WP 96-10182
Dark spots and flecks were frequently recognized on the surface and within non-preservative treated hard- and softwood test stakes placed in soil contact. Energy dispersive X-ray microanalysis in conjunction with electron microscopy showed the flecks to be composed primarily of manganese dioxide. Detailed transmission electron microscopy observations indicated intrusion of manganese into the wood ...
G F Daniel, T Nilsson, J Volc
Effects of terpene compounds on the growth and peroxidase activity of Phellinus pini
1979 - IRG/WP 2130
The volatile compounds myrcene, limonene, a-pinene, a-terpineol, D-fenchol and 1,8-cineole, present in the oleoresin fraction of coniferous wood, were tested individually and as mixtures for their effect on the growth and peroxidase activity of cultures of six strains of Phellinus pini (Fr.) A.Ames and one strain of Phellinus chrysoloma (Fr.) Donk (Phellinus pini var. abietis (Karst.) Pilát). Phe...
L Väisälä
Screening of lignin peroxidase from white-rot fungi
1998 - IRG/WP 98-10274
Since lignin peroxidase was first found in the ligninolytic culture of Phanerochaete chrysosporium, it has been regarded as an enzyme responsible for the lignin biodegradation and studied comprehensively. As the results of these studies its unique characteristics have been revealed. On the other hand, the features of lignin peroxidase produced by other white-rot fungi have not been studied so much...
I Momohara
An electron spin resonance study of manganese changes in wood decayed by the brown-rot fungus, Postia placenta
1988 - IRG/WP 1359
Electron spin resonance (ESR) spectrometry was used to examine wood decay by the brown-rot fungus, Postia placenta. Wood slivers of Douglas-fir, white fir, redwood, sweetgum and yellow poplar were incubated for 4 weeks in custom-made quartz ESR tubes with or without Postia placenta. In all wood species without fungus, a weak partially resolved signal (about g=2, presumably due to manganese) was de...
B Illman, D C Meinholtz, T L Highley
A comparison of inductively coupled plasma spectroscopy and neutron activity analysis for the determination of concentrations in wood
1993 - IRG/WP 93-10048
As wood decays the ionic composition changes, with increases often being seen in the concentrations of Ca, Mg, Fe, Mn and sometimes K. The concentration of eight cations in red spruce sapwood and heartwood samples was determined independently by inductively coupled plasma spectroscopy (ICP) and by neutron activation analysis (NAA) as part of an effort to standardize our analytical procedures and c...
J Jellison, J Connolly, K C Smith, W T Shortle
Controlling the sapstain fungus Ceratocystis coerulescens by metabolites obtained from Bjerkandera adusta and Talaromyces flavus
1993 - IRG/WP 93-10024
Sapstain causes severe damage to wood and wood products, posing a major economic problem for the wood industry. The purpose of this study was to determine if metabolites from Bjerkandera adusta and Talaromyces flavus would (1) decolorize stain in wood caused by Ceratocystis coerulescens and (2) prevent sapstain by Ceratocystis coerulescens. We studied the interaction of the sapstain fungus Ceratoc...
S C Croan, T L Highley
Regulation of hyphal sheath formation iron-chelator production by the brown rot fungi Gloeophyllum trabeum and Postia placenta
1994 - IRG/WP 94-10074
Hyphal sheath formation and iron-chelator production by liquid cultures of the brown rot fungi Gloeophyllum trabeum and Postia placenta were monitored over time. The effects of incubation at various temperatures, pH's and concentrations of iron, manganese and nitrogen were determined. Hyphal sheath production was observed microscopically and quantified with JAVA video analysis. The hyphal...
Y Chen, J Jellison
EPR observation of Mn(II) in decayed wood
2002 - IRG/WP 02-10443
Preliminary Electron Paramagnetic Resonance (EPR) studies of copper/chromium (CC) treated spruce wood, subjected to laboratory soil decay test showed increased intensities of Mn(II) EPR signal in partially decayed specimens. Several reasons might be responsible for this phenomenon: Mn(II) could originate from the wood after being released from the typical ligand binding, from reduction of other Mn...
M Petric, M Humar, P Kalan, F Pohleven, U Schoknecht, M Šentjurc
Surface protection of wood with titanium, zirconium and manganese compounds
2005 - IRG/WP 05-30376
Thin radiata pine veneers were treated with a range of titanium, zirconium and manganese compounds and exposed to natural weathering. Veneer weight and tensile strength losses were used to assess the protective efficacy of the compounds. The oxidative manganese compounds potassium permanganate and manganic acetate restricted both weight and tensile strength loss of treated wood veneers during weat...
K J Schmalzl, P D Evans
Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium
1988 - IRG/WP 1364
The degradation of lignin in native lignocellulosic substrates by white rot fungi is poorly understood. Biochemical studies have shown the involvement of a number extracellular ligninolytic enzymes released by white rot fungi which are capable of the oxidative conversion of DHP's (lignin model compounds) in vitro, but to date conclusive evidence for occurrence of these enzymes in wood und...
G F Daniel, T Nilsson, B Pettersson
Oxidative stress and lignin peroxidase production in Phanerochaete chrysosporium
2008 - IRG/WP 08-10655
In Phanerochaete chrysosporium liquid cultures, the induction of lignin peroxidases is directly related to accumulation of reactive oxygen species at the mitochondrial level. In this study, we demonstrate that the expression of the mitochondrial thiol-related antioxidant system is not directly coupled to the LiPs expression. When the antioxidant systems are not able to cope with ROS accumulation, ...
M Morel, L Diss, C Fourrey, M Chalot, M Droux, J P Jacquot, E Gelhaye
Wood preservative obtained by recycling – preliminary selection of leaching inhibitor
2008 - IRG/WP 08-50252
Based on recycling of used household batteries, a process was developed to enable the recovery of major manufacturing components, leading to a solution of zinc (32 g.l-1) and manganese (15 g.l-1) salts, free of heavy metals. The potential use of the above solution as wood preservative was demonstrated by means of assays both of fungicidal activity -using white and brown rot fungi- and of insectici...
C Ibáñez, C Mantero, G Cecchetto, M Rabinovich, M Cerdeiras