IRG Documents Database and Compendium


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Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP I: Accelerated laboratory decay study
2006 - IRG/WP 06-20332
The effects of Chlorothalonil (CTN) and Butylated Hydroxy Toluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and BHT is being evaluated for its synergistic effects with CTN. Results from an accelerated decay test will be presented. The...
G T Kirker, M L Prewitt, S V Diehl


Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP II: Results from field studies
2007 - IRG/WP 07-30429
The effects of Chlorothalonil (CTN) and Butylated Hydroxytoluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and the non-biocidal BHT was evaluated for its synergistic effects with CTN. ACQ-C was a positive control and untreated SYP sta...
G T Kirker, M L Prewitt, S V Diehl


Microbial Community Analysis Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis: Field Study Results
2008 - IRG/WP 08-20377
The effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on the fungal community in southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis. Field stakes treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1 and 0.25% CTN, and 0.1 and 0.25% CTN combined with 2% BHT were installed with untreated controls in field s...
G T Kirker, S V Diehl, M L Prewitt, W J Diehl


Towards Understanding the Biology of Wood Decay
2010 - IRG/WP 10-10739
Our previous research has focused primarily on ways to identify the wood decay fungi and microbial community. We continue to explore this complex and dynamic community and its interactions through microbial community ecology studies, gene expression interactions and proteomics. However, in order to better understand the mechanisms of fungal decay, we have sequenced the genome of a copper tolerant...
J Tang, K Jenkins, L Parker, S V Diehl


Microbial Community Analysis of Naturally Durable Wood in an Above Ground Field Test
2014 - IRG/WP 14-10826
This paper presents preliminary results of an above ground field test wherein eight naturally durable wood species were exposed concurrently at two sites in North America. Surface samples were taken at regular intervals from non-durable controls and compared to their more durable counterparts. Terminal Restriction Fragment Length Polymorphism was performed to characterize the microbial (bacteria, ...
G T Kirker, S V Diehl, P K Lebow


Report on International Conference on Marine Biodeterioration, Goa, India, January 1986
1986 - IRG/WP 4127
At the International Conference on Marine Biodeterioration - Advanced Techniques Applicable to the Indian Ocean, there were a number of papers relevant to the aims of Working Group IV of IRG. Abstracts of these papers and a brief commentary on the conference are presented....
S M Cragg


Microbial biofouling of 10-40% naphthalene in creosote treated and untreated wooden pilings in the marine environment
1978 - IRG/WP 442
R R Colwell, P L Fish, D A Webb, A J Emery


Three dimensional computer representations of growth of microbial populations in wood
1984 - IRG/WP 1243
Creosoted distribution poles inoculated with either Lentinus lepideus, biological control organisms including Triochoderma or combinations of both were extensively sampled to monitor the spread of organisms. A computer program which enabled the results to be portrayed in a three dimensional graphic form was developed and is illustrated. Results showed that computer mapping of this type usefully en...
A Bruce, B King, C Bruce, G M Smith


Initial microbial colonisation of CCA-treated, creosote-treated and untreated Pinus sylvestris L. blocks in seawater
1996 - IRG/WP 96-10171
CCA-treated, creosote-treated and untreated Pinus sylvestris sample blocks were immersed in a seawater through-flow system for periods of between 1 and 265 hours. Samples were fixed immediately on removal and examined both qualitatively and quantitatively using the scanning electron microscope. This study of primary microbial colonisation showed differences between treatments in the diversity of m...
K Sturgess, A J Pitman


Case histories involving attempts at identifying infestations, determining the source and controlling the Formosan subterranean termite in Atlanta, Georgia USA
2000 - IRG/WP 00-10342
The University of Georgia Department of Entomology and the Georgia State Department of Agriculture have been involved in identifying and working with Pest Control Operators and homeowners dealing with Formosan subterranean termite infestations in the Atlanta, Georgia metropolitan area since 1993. Our involvement with seven separate infestations is outlined in this paper in the form of case histori...
B T Forschler, J Harron, T M Jenkins


Moisture uptake and volumetric swelling as probable factors also affecting leaching in CCA-treated wood. A preliminary study of treated Eucalyptus saligna sapwood
2003 - IRG/WP 03-30318 IRG/WP 03-30318
A number of factors affecting leaching of CCA from treated wood have been variously investigated and discussed. However, the possible effects of moisture movement in treated timbers on leaching have not received attention yet. The present study examines and compares moisture uptake and volumetric swelling in CCA-treated and untreated sapwood of Eucalyptus saligna, and tries to establish a correl...
R Venkatasamy


Microbial breakdown mechanisms. Mini-Symposium at the 18th IRG meeting, Honey Harbour, Ontario, Canada
1987 - IRG/WP 1327
An understanding of how micro-organisms cause chemical alternation to wood is essential for effective wood preservation. Investigation of the Microbial Breakdown of wood is a very dynamic research field and is increasing in importance as several biocides are being examined as alternativesw to classic wood preservatives. These biocides often do not possess broad spectrum activity. It is esential th...
L E Leightley


Microbial decay of an archaeological wood
1994 - IRG/WP 94-10053
A light and transmission electron microscopic investigation of an archaeological wood was undertaken to determine the cause of its deterioration. The wood came from a bulwark constructed in early 1100 in the lake Tingstäde Träsk on the island Gotland in Sweden. The samples of the wood, which was identified as Pinus sylvestris, were taken from a depth of 0.85 m below the bottom level. The wood wa...
A P Singh, T Nilsson, G F Daniel


Microscopic characteristics of microbial attacks of CCA-treated radiata pine wood
1993 - IRG/WP 93-10011
Light and electron microscopic observations were made of CCA-treated radiata timbers, which had been placed in service in a vineyard soil as supporting poles and as part of a house pile, to determine the cause of their deterioration. The house pile had failed in service after between 9 and 13 years and was of particular interest because decay was more severe in deeper regions than at the surface a...
A P Singh, R N Wakeling


Microbial decomposition of salt treated wood
1993 - IRG/WP 93-50001-22
Specialized microorganisms which are able to convert fixed inorganic preservatives from treated wood into water soluble components are investigated. A number of brown rot fungi like Antrodia vaillantii have been isolated from cases of damage and examined under unsterile conditions with CCA-, CCB-, CCF- and CC-treated wood at retention levels of at least 50% higher than recommended for wood in grou...
R-D Peek, I Stephan, H Leithoff


Environmental risk assessment of treated wood - A mesocosm study
2005 - IRG/WP 05-50224-8
In order to evaluate the environmental impact of treated poles, we have developed physical models (mesocosms) containing around 250 kg of natural soil and a treated pole. On the top of each mesocosm, a spraying mechanism, which can distribute ultrapure water through several nozzles, was built. On the lower part of each mesocosm, a small tank, which can contain some litres of leachate, was placed...
P Marchal, F Poly


Microbial fouling of 10-40% naphthalene in creosote treated and untreated wooden pilings in the marine environment - A progress report
1980 - IRG/WP 455
In June 1978, Document No: IRG/WP/442 entitled 'Microbial Biofouling of 10-40% Naphthalene in Creosote Treated and Untreated Wooden Pilings in the Marine Environment" was released to the IRG members as a report on the early results of a long term (about 5 years) study of whether the naphthalene content of creosote was importart in protecting wood pilings in the marine environment from bor...
R R Colwell, A J Emery


Susceptibility of painted wood to discolouring fungi - influence of binder, solvent and surfactant
1992 - IRG/WP 92-3714
Previous studies have shown that the basic composition of paints, without fungicide addition, can significantly contribute to the varying microbial susceptibility of painted wood. Previously, non-weathered water-borne acrylic paints applied to wood without a primer were shown to be more susceptible to mould attack than solvent-borne alkyd paints. The present study was initiated to reveal if this d...
J Bjurman, C Herder


Fungal colonisation of the keelson and associated structures of a nineteenth century wooden frigate: Concepts of community structure and development
1994 - IRG/WP 94-10072
The early stages of microbial colonization and succession of wooden surfaces exposed to the sea have been extensively studied as have the community structures of archaeological timbers subjected to submergence and then retrieval from the sea. The frigate UNICORN, a largely intact 19th century wooden ship based in Dundee, Scotland, provides the microbial ecologist with a unique opportunity to study...
N A White, J W Palfreyman, G M Smith


Microbial tolerance and biodetoxification of organic and organometallic biocides
1990 - IRG/WP 1464
Ten organic and organometallic biocides were assessed for toxicity towards a range of wood decay and spoilage fungi. Minimum inhibitory concentrations indicated selective toxicity of biocides towards particular fungal types. Certain species of fungi demonstrated tolerance to a range of biocides. Further tests using the biocides in soil and wood enrichment cultures resulted in isolation of highly t...
P A Briscoe, G R Williams, D G Anderson, G M Gadd


Aboveground Microbial Decay Test of Biocide Treated and Untreated Wood Exposed to Danish and Humid Tropical Climates
2004 - IRG/WP 04-20306
A co-operative study was initiated to compare the aboveground field decay test of untreated and preservative-treated timbers under Nordic (Danish) and a representative humid tropical (Malaysian) environment according to CEN standard method. Matched specimens of untreated and both preservative-impregnated and surface-treated wood were simultaneously exposed at The Danish Technological Institute (DT...
A H H Wong, N Morsing, K H Henriksen, S Ujang


An investigation of the factors influencing the rate of deterioration of timber samples due to microfungi in laboratory tests
1997 - IRG/WP 97-20116
An investigation was performed into the comparative performances of test 1 (vermiculite burial) and test 2 (soil burial) as described in ENV 807. Two test preservatives were used - copper naphthenate and propiconazole. Copper chromate was included as a reference preservative. The wood species used was beech (Fagus sylvatica). Test 1 was performed using both a mixed inoculation of the five stipulat...
I J Herring, D J Dickinson, S M Gray, J K Carey


Microbial decay in an extremely durable Malaysian hardwood Belian (Eusideroxylon zwageri) - an overview
1997 - IRG/WP 97-10216
The heartwood of Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods, and is therefore a choice timber for soilcontact use in the humid tropics such as untreated telecommunication poles, fence posts and railway sleepers. The high microbial resistance of the wood is evidenced by the mere superficial degradation observed in some pole samples after 20 yea...
A H H Wong, A P Singh


Fouling assemblage development on copper-chromium-arsenic-treated timber submerged in European waters
2002 - IRG/WP 02-50181
The effect of the anti-marine-borer timber preservative CCA on community development of non-target marine fouling animals was investigated. Panels of Scots pine treated to target retentions of 12, 24 and 48 kg CCA per m3 of wood, plus untreated controls were submerged at seven coastal sites (Portsmouth, UK; La Tremblade (2 sites), France; Ria Formosa, Portugal; Sagres, Portugal; Kristineberg, Swed...
C Brown, R J Eaton, S M Cragg, P Goulletquer, A Nicolaidou, M J Bebianno, J Icely, G F Daniel, T Nilsson, A J Pitman, G Sawyer


The potential application of rapid gas-chromatographic assay of microbial respiration to the monitoring of wood decay in field trial situations
1983 - IRG/WP 2196
Gas chromatographic detection of microbial activity (C02 production) within stakes in a field trial situation would appear to provide a sensitive, non-destructive and relatively rapid method for the quantitative assessment of preservative treatments. Most consistent results were obtained when stakes were removed from the soil, washed, saturated with water and incubated in sealed PVC tubes at 25°C...
M A Line


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