IRG Documents Database and Compendium


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A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl


The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari


Identification and inhibitory effect of volatiles from different ages of a Trichoderma aureoviride culture on selected wood decay fungi.
1995 - IRG/WP 95-10110
The ability of a Trichoderma sp. to produce volatile organic compounds (VOCs) over a four week period of growth was examined and the inhibitory effect of these volatiles against four selected basidiomycetes over the same period was assessed. After trapping, on tubes filled with chromatography packing material, VOCs were analysed on an integrated automated thermal desorbtion mass spectrometer syste...
A Bruce, A Kundzewicz, R E Wheatley


Efficacy of some fungicides against mold on freshly cut beech sawn wood
2006 - IRG/WP 06-30403
The quality class of molded wood is lowered and this can result in financial losses due a decrease in its trade value. Currently beech wood is often used without steaming it first and it moulds in the course of drying. The aim of this study was to identify kinetics of beech wood molding and effectiveness of action of chosen modern pro-ecological wood preservatives applied in the protection of fres...
A Fojutowski, A Kropacz


Moldicidal properties of seven essential oils
2006 - IRG/WP 06-30404
When wood and wood products are exposed to moisture during storage, construction or while in-service, mold growth can occur in 24 to 48 hours. Mold growth could be suppressed or prevented if wood was treated with an effective mold inhibitor. The objective of this study was to evaluate the mold inhibiting properties of natural plant extracts such as essential oils. Seven essential oils were evaluat...
V W Yang, C A Clausen


Identification of fungi colonising coated and modified wood exposed outdoors using sequencing and T-RFLP profiling
2006 - IRG/WP 06-20326
Wood decay and staining fungi are known to colonize coated and modified wood. Rapid and reliable ways of identifying fungi on coated and modified wood may improve diagnostics and product development. In this work nine fungi growing in painted wood panels exposed outdoors at a test field in Uppsala, Sweden, were identified using sequencing and T-RFLP (Terminal Restriction Fragment Length Polymorphi...
U Råberg, J Bijelovic, C J Land, S Bardage, N Terziev


Wood decay fungi from New Zealand ‘leaky’ buildings: PCR identification and laboratory decay tests of wood preservative-treated Pinus radiata (Part 1)
2007 - IRG/WP 07-10620
Fungi colonising Pinus radiata D. Don framing timber of ‘leaky’ New Zealand buildings were isolated to produce pure cultures. Mycelia from these cultures on agar media were collected to extract DNA. To identify the fungi to the species level, polymerase chain reaction (PCR) with primer pairs ITS1-F and ITS4 were performed followed by sequencing of the internal transcribed spacer (ITS) region. ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Wood printing plates from the Dürer era – species identification and anatomical study
2007 - IRG/WP 07-20364
Anatomical examinations serve to answer questions regarding the artistic technique and authenticity of art objects made of wood. The wood of 28 printing plates from the 15th and 16th centuries from the Berlin Kupferstichkabinett were investigated. Seven of these which date from the Dürer era (Albrecht Dürer, 1471-1528) were examined in more detail. With one exception, the plates from the Dürer ...
M Penkuhn, W Unger


Mold resistance of heat-treated wood
2007 - IRG/WP 07-40358
The resistance of heat-treated sugi sapwood against three mold fungi was evaluated. Wood specimens were first subjected to heat treatments at 180 and 220ºC for 2 and 4 hours. Mold growth on the surface of the specimens was measured 1, 2, 4 and 6 weeks after the inoculation with the fungi in laboratory conditions. Laboratory decay tests were performed using two brown and white-rot fungi along with...
S N Kartal


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


Control of Moisture Content in Wood Materials for Mold Growth
2007 - IRG/WP 07-10630
Mold growth on wood materials is a major concern for house owners, as it can cause health and aesthetic problems. Mold fungi, present everywhere, usually appear as black or gray patches on wood surfaces in humid environments, and are common in houses. Like any living organism, molds need food, water, oxygen, and a specific temperature range in order to grow. To avoid mold growth on wood materia...
Dian-Qing Yang


Laser stain removal of fungus-induced stains on paper-based cultural relics
2007 - IRG/WP 07-40389
Laser techniques has been developed and applied in commercial use for 40 years, but it has not been used successfully to remove stains from paper-based cultural relics . The purpose of this study was to investigate the paper cleaning by using a technique of Nd-YAG laser. Artificial stained papers were inoculated with fungi and then were treated at different laser cleaning parameters, in order to f...
Tsang-Chyi Shiah, Han Chien Lin


Wood decay fungi from New Zealand leaky buildings – PCR identification (Part 2) and aerial spore trapping
2008 - IRG/WP 08-10649
Prior to this study, it was not know which species of decay fungi caused decay in New Zealand leaky buildings. Use of molecular biology methodology, polymerase chain reaction (PCR), and subsequent DNA sequencing, as well as classical mycological techniques based on morphology, has enabled identification of decay fungi and has provided insight into their relative importance based on isolation frequ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Water Absorption of Various Building Materials and Mold Growth
2008 - IRG/WP 08-10657
Mold growth is a major problem for house owners, as it often occurs on the surface of building materials in damp houses. The principal method to control mold growth on building materials is to prevent water penetration into the materials. A study was recently conducted to determine water absorption rates of different wood species and panel materials used as building materials, when water intrusi...
Dian-Qing Yang


Evaluation and identification of extractives from Iranian walnut (Juglans regia L.) by GC/MS technique for protection of non-decay resistant species
2008 - IRG/WP 08-10670
The use of walnut extractives is interest as environment friendly wood preservatives in the wood preservation industry. In this study, organic solvents were used to remove extractives from Iranian walnut heartwood samples. The solvent was removed by rotoevaporation, and Identification of chemical compounds in the Walnut extractives was done by using GC/MS technique. The toxicity of various extract...
S K H Hashemi, D Parsapajouh, H Khademi Eslam


Molecular Methods: a Reliable Tool for the Identification of Wood Decay Fungi in Construction Timber
2008 - IRG/WP 08-20386
In the present study, we tested the practical value of several DNA-based methods to identify at the species level the most common wood-decaying fungi infecting buildings. We successfully extracted and amplified fungal DNA from pure cultures of twelve species of wood-inhabiting fungi, from oak and pine wood infected in laboratory with known strains, and from unknown field samples of wood collected ...
M Maître, M Kutnik, I Le Bayon, L Harvengt


Wood Decay Research Using Molecular Procedures, What Can It Tell Us?
2008 - IRG/WP 08-10678
There are many unanswered questions in wood decay and protection research, and no single research technique will ever answer all of these questions. The process of wood decay is a very complex series of biochemical and chemical reactions that are heavily influenced by the hundreds of bacterial and fungal species found on environmental samples of wood. There are a variety of molecular techniques b...
S V Diehl, M. L Prewitt, Young-Min Kang, L Mangum, J D Tang


Aislamiento, identificación y evaluación enzimática de hongos de pudrición de madera de la Región de los Lagos
2008 - IRG/WP 08-10680
So far, systematic investigations have not been developed to determine the fungal diversity associated with wood in Chile. In addition, little is known about the mycoflora capabilities or their enzymatic processes in biotechnology although different research has hinted at the great potential of these microorganisms in various industrial processes. The aim of this work is to isolate, identify and e...
R Ortiz, J Navarrete, C Oviedo, R Blanchette


Mold fungi - Major impediments to cleaning/repairing flood and rain-damaged homes
2008 - IRG/WP 08-40427
Damage to homes by hurricanes that make landfall in the south and southeastern United States, or sub-tropical and tropical regions, during the hot, humid summer months is compounded by the growth of mold fungi on wetted building materials. Most homeowners are better equipped to deal with construction problems associated with storm damage than to control the growth of mold fungi. Following hurrican...
T L Amburgey


Isolation and identification of stain fungi and mould fungi on bamboo wood in China
2009 - IRG/WP 09-10703
One hundred and seventeen isolations were isolated from 150 specimens of mildewed or stained bamboo wood and bamboo products from 9 locations in north, south and central China. The molecular (ribosomal DNA Internal Transcribed Spacer, rDNA-ITS) and morphological identification results showed these fungi could be categorized 2 phyla, 2 classes, 4 subclasses, 10 orders, 15 families, 22 genera, 56 sp...
Xingxia Ma, Mingliang Jiang, Daochun Qin


Wart Morphology can Distinguish White Cypress Pine from the Less Durable Species, Black Cypress Pine
2009 - IRG/WP 09-20406
White cypress pine (Callitris glaucophylla) wood is durable enough to be used outdoors, but occasionally there are reports of its premature failure in ground contact, which may be due to its substitution by the less durable species, black cypress pine (C. endlicheri). It has been difficult to prove this, however, because the woods of both species are very similar in structure and cannot be separat...
R Heady, R Cunningham, P Evans


Mold-resistance Effect of Bamboo Wood Treated with CCC-organic Complexes
2009 - IRG/WP 09-30514
Mold resistant effect of CCA, ACQ, CuAz, CCC and the compound of CCC and propiconazole were researched on bamboo wood of Phyllostachys pubescens were reported in this paper. Results showed that all of the test fungicides could protect bamboo wood better from Penicillam citrinum than from Trichoderma viride and Aspergillus niger. The complex of CCC and propiconazole had the best resisting effect on...
Sun Fangli, Yang Le, Chen Anliang, Bao Binfu, Li Qiao


Assessment of decay risk of airborne wood-decay fungi
2012 - IRG/WP 12-10787
The decay risk of airborne wood-decay fungi was investigated by using an air sampler. Japanese cedar disks measuring about 8 cm in diameter and 3 mm in thickness with moisture content at about 100 % were placed in a “BIOSAMP” air sampler and exposed to 1000 liters of air. Air sampling was carried out from June to September at the same sampling site in Tsukuba, Japan. The exposed disks were the...
I Momohara, Y Ota, K Sotome, T Nishimura


Stained wood: The identification of natural dyes and mordants in wooden works of art
2010 - IRG/WP 10-20434
On the basis of current knowledge only natural-colored wood species crafted to veneers were used for the decorative design of furniture and wooden interior in classical antiquity.First applications of artificially stained wood can be dated to the late Middle Ages. In the Renaissance wooden works of art were manufactured by using intarsia of natural-colored, fungus-stained and artificially stained ...
A Unger, W Unger


Drying Rates and Mold Growth on Various Building Materials under Different Environmental Conditions
2010 - IRG/WP 10-20454
Mold growth on building materials is a major problem for homeowners. The most suitable method to control mold growth on building materials is to utilize design features, construction tools and practices that prevent moisture accumulation, and keep the wood as dry as possible. In order to achieve this, engineers and homebuilders have to know the effects of various temperature and moisture conditi...
Dian-Qing Yang


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