Your search resulted in 93 documents. Displaying 25 entries per page.
What can DNA fingerprinting, aggression tests and morphometry contribute to the identification of colonies of the Formosan subterranean termite
2000 - IRG/WP 00-10371
Multilocus DNA fingerprinting, aggression tests and morphometry were compared to evaluate their potential for the identification of colonies of the Formosan subterranean termite, Coptotermes formosanus (Isoptera: Rhinotermitidae) in Hawaii. DNA fingerprinting separates the termites from all studied collection sites. Since the genetic similarity between termites from different collection sites lies...
C Husseneder, J K Grace
Molecular studies on house rot fungi by RAPD-PCR
1997 - IRG/WP 97-10195
For genetic information and in view as a possible diagnosis method various isolates of the dry rot fungus, Serpula lacrymans, the wild merulius, S. himantioides, and the cellar fungus, Coniophora puteana, from Asia, Europe and USA were investigated by the polymerase chain reaction (PCR) based technique of randomly amplified polymorphic DNA (RAPD). The banding patterns obtained revealed for S. lacr...
O Schmidt, U Moreth
rDNA-ITS sequence of Serpula lacrymans and other important indoor rot fungi and taxon-specific priming PCR for their detection
1999 - IRG/WP 99-10298
Taxon-specific priming polymerase chain reaction (TSPP) is a powerful molecular tool for fungal diagnosis. For its application to indoor rot fungi, the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) of the main fungal species causing wood rot in European buildings was amplified with the polymerase chain reaction (PCR). The ITS region was sequenced. The complete sequences are present...
O Schmidt, U Moreth
Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt
A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl
Molecular Methods: a Reliable Tool for the Identification of Wood Decay Fungi in Construction Timber
2008 - IRG/WP 08-20386
In the present study, we tested the practical value of several DNA-based methods to identify at the species level the most common wood-decaying fungi infecting buildings. We successfully extracted and amplified fungal DNA from pure cultures of twelve species of wood-inhabiting fungi, from oak and pine wood infected in laboratory with known strains, and from unknown field samples of wood collected ...
M Maître, M Kutnik, I Le Bayon, L Harvengt
Wood Decay Research Using Molecular Procedures, What Can It Tell Us?
2008 - IRG/WP 08-10678
There are many unanswered questions in wood decay and protection research, and no single research technique will ever answer all of these questions. The process of wood decay is a very complex series of biochemical and chemical reactions that are heavily influenced by the hundreds of bacterial and fungal species found on environmental samples of wood. There are a variety of molecular techniques b...
S V Diehl, M. L Prewitt, Young-Min Kang, L Mangum, J D Tang
What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
Molecular techniques are now routinely used in the identification, detection and analysis of wood degrading organisms. An overview of some of the early work on nucleic acid isolation and characterization will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of pre...
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace
Molecular identification of marine borers causing wood deterioration on marine facilities in Korea
2019 - IRG/WP 19-10955
The deterioration factors of wood which was used in the marine environment, Tongyeong (South Coast) and Incheon (West Coast) in Korea, were investigated in order to develop technologies for safe and long-term use of timber in ocean facilities.
The marine microorganisms isolated by damaged wood were identified as morphological Chelura sp. and Teredo sp. As a result of DNA sequencing, it was confir...
Sae-Min Yoon, Min-Ji Kim, Won-Joung Hwang, Yong-Seok Choi, Dong-Won Son
Data sheet on wood-boring insects. Apate monachus Fabricius. 2. Position systématique, nomenclature, identification et distribution - Espèces végétales attaquée
1981 - IRG/WP 1105
R L A Damoiseau
Penichroa fasciata (Stephens) (Col. Cerambycidae) a pest in wood materials
1988 - IRG/WP 1365
Penichroa fasciata (Stephens) (Col. Cerambycidae) is found to be a frequent pest occurring in hardwood in storage in Italy. This paper reports the characteristic for identification, biological features, distribution and timber liable to attack....
A Gambetta, E Orlandi.
The identification and preservative tolerance of species aggregates of Trichoderma isolated from freshly felled timber
1992 - IRG/WP 92-1553
The surface disfigurement of antisapstain treated timber by preservative-tolerant fungi remains a major problem in stored timber. Identification of a range of isolates of Trichoderma based on microscopic morphological characteristics was found to be imprecise due to the variable nature of this organism. In addition, studies to compare visual (morphological) characteristics of these isolates with t...
R J Wallace, R A Eaton, M A Carter, G R Williams
The use of C CP/MAS NMR in the chemical identification of decayed and undecayed, tropical timber species
1984 - IRG/WP 1224
13C CP/MAS NMR was found to be an extremely powerful tool for elucidating the chemical composition of Eucalyptus maculata, Pinus elliottii and Alstonia scholaris. The differences in lignin composition were different for each timber and discussed in relation to decay caused by soft-rot and white rot fungi. In particular the presence of syringyl and guaiacyl lignin types are discussed.
L E Leightley
On the problem "House Longhorn Beetle" in hardwoods and an aid to distinguish between Hylotrypes bajulus and Hesperophanes cinereus larvae
1981 - IRG/WP 1141
S Cymorek
Data sheet on wood-boring insects: Ptilinus pectinicornis (Linnaeus)
1987 - IRG/WP 1334
S Cymorek, M-M Serment
Isolation and identification of the fungal flora in treated wood
1976 - IRG/WP 144
J F Levy
Identification card for Coriolopsis polyzona (Pers.) Ryv
1977 - IRG/WP 161
A Ofosu-Asiedu
Molecular Microbiology and Wood Protection
2009 - IRG/WP 09-20416
During the last decade, the development of molecular tools has allowed significant advances in environmental microbiology. Such techniques have proved to be invaluable tools for the qualitative and quantitative description of environmental microbial communities. The aim of this paper, written for non-specialized readers, is to discuss the possible applications of such tools in wood preservation fi...
E Gelhaye, M Morel
Molecular studies on isolates of Serpula lacrymans
1989 - IRG/WP 1421
The major protein species present in detergent extracts of 14 different Serpula lacrymans isolates have been compared, by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with a standard strain, viz. Serpula lacrymans FPRL 12C. Following silver staining of SDS gels the major protein species identified in 12 isolates were similar to those found in the standard strain. However ...
A Vigrow, D Button, J W Palfreyman, B King, B M Hegarty
Growth of two selected sapstain fungi and one mould on chitosan amended nutrient medium
2003 - IRG/WP 03-10466
In vitro studies were undertaken to investigate the effect of chitosan on growth of Leptographium procerum, Sphaeropsis sapinea and Trichoderma harzianum. Chitosan was tested at three molecular weight (MW) ranges and different concentrations formulated as either a powdered suspension or as a solution.
The results generally showed that low MW chitosan produced a greater inhibitory effect on growt...
C Chittenden, R N Wakeling, B Kreber
Isolation of the dry rot fungus, Serpula lacrymans, from the forests of the Himalayan Foothills
1995 - IRG/WP 95-10129
Previous expeditions to the Himalayas (Singh 1993, 1994) have reported on the presence in this area of the dry rot fungus Serpula lacrymans. However, attempts to isolate the organism from material brought back from these expeditions were not successful. In this paper we report on the isolation of Serpula lacrymans from spores and cord material brought back from the Narkanda region of the Himalayas...
N A White, J W Palfreyman, J Singh, S Singh
The effect of low molecular weight chelators on iron chelation and free radical generation as studied by ESR measurement
2000 - IRG/WP 00-10367
The focus of this work was to improve our current knowledge of the non-enzymatic mechanisms involved in brown-rot decay. Electron Spin Resonance (ESR), also known as Electron Paramagnetic Resonance (EPR), is an attractive technique for the identification and study of chemical species containing unpaired electrons (such as radicals and certain transition metal species). ESR spin-trapping techniques...
Yuhui Qian, B Goodell
Isolation and identification of bacteria from degraded wood - A progress report
1986 - IRG/WP 192
During attempts to isolate tunnelling bacteria in pure culture, physical separation from other bacteria was successfully achieved by the use of cellophane. Cellophane was used in liquid cultures as a substrate for tunnelling bacteria that were present in a mixture with other bacteria. By tunnelling into cellophane, TB were physically removed from contaminating bacteria which could then be removed ...
J A Drysdale, P J Ruland, J A Butcher
Isolation and identification of bacteria from CCA-treated eucalypt power transmission poles
1987 - IRG/WP 1317
Bacteria have been isolated from CCA treated Eucalyptus maculata poles which possessed characteristic bacterial decay patterns. Cultures were isolated on a number of different media which had been inoculated with a splinter of degraded wood. Primary tests indicated that most isolates belonged to the genera Pseudomonas and Bacillus. The species Pseudomonas cepacia was most frequently isolated....
G A Willoughby, A C Hayward, L E Leightley
A novel defaunation method of the protozoa to investigate cellulose metabolism in Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae)
1994 - IRG/WP 94-10050
The largest protozoa in the hindgut of workers of Formosan subterranean termite, Coptotermes formosanus Shiraki, was selectively eliminated by forced-feeding on low-molecular weight cellulose (LC) with a mean DP of 17. Although one week's feeding on LC caused perfect disappearance of Pseudotrichonympha grassii Koidzumi, the selective defaunation method itself had no detrimental effect on ...
T Yoshimura, K Tsunoda, M Takahashi