IRG Documents Database and Compendium


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The uses of Molecular techniques in studying Australian subterranean termites Genus Coptotermes
2008 - IRG/WP 08-10669
This study focuses on applying molecular tools in studying the phylogeny of the Coptotermes, especially from the Australian region, by using a combined data set of three mitochondrial genes, viz. 16S, COII, COI, comprising a total of about 2000 base pairs. This study will address these following specific questions: i. Does molecular phylogenetics reveal any new species and show any previously ...
H M Badawi, B M Ahmed (Shiday), J R J French, M P Schwarz


Termite taxonomy and distribution with particular reference to climate change in Africa
2010 - IRG/WP 10-10738
Termites constitute an integral component of various ecosystems in Africa. Termites are also amongst the most difficult of the insects to study because of their cryptic behaviour. There are around 2600 species of termites (Isoptera) in 280 genera which have been described worldwide and about 39% of the total termite species are found in Africa. Some termite species are well known pests of agricul...
P O Y Nkunika, B M Ahmed Shiday, G W Sileshi, J R J French, P Nyeko, S Jain


What molecular biology can tell us about the biodegradation of lignocellulose: the utilization of molecular techniques for the detection, identification and enhanced understanding of wood degrading organisms
2013 - IRG/WP 13-20528
Molecular techniques are now routinely used in the identification, detection and analysis of wood degrading organisms. An overview of some of the early work on nucleic acid isolation and characterization will be followed by a discussion of the power of sequencing and other procedures for better understanding: the mechanisms involved in the biological degradation of wood, the metabolic basis of pre...
J Jellison, B Goodell, G Alfredsen, D Eastwood, G Daniel, S M Cragg, J K Grace


The dry rot fungus Serpula lacrymans. Examples of attack and remedial treatment
1988 - IRG/WP 1347
The film deals with several aspects of dry rot attack and eradication in buildings. The detailed biology and morphological charasteristics of the fungus are portrayed. The various forms of mycelial growth, the role of the strands in the nourishment and spread of the fungus, as well as the many types of fruitbody formation are outlined. Environmental and nutritional requirements of the fungus as we...
G Buchwald, B M Hegarty, W Metzner, R Pospischil, H Siegmund, P Grabow


Improved techniques designed for evaluation of fungicides in soil for control of dry rot fungus Serpula lacrymans
1985 - IRG/WP 2238
Improved techniques provide a laboratory method for the evaluation of chemicals in soil for control of dry rot fungus Serpula lacrymans. Results with their application to three chemicals were reported. These techniques are useful to eliminate chemicals lacking the necessary toxicity and weatherbility for dry rot control when the chemicals have been applied to the soil....
M Takahashi, K Nishimoto


Current techniques for screening initial formulations against Basidiomycetes and soft rot
1978 - IRG/WP 2103
J D Thornton, H Greaves


Recycling of pressure impregnated timber and preservatives - incineration techniques
2001 - IRG/WP 01-50166-19
The object of the recycling research projects is to determine the technical and economical requirements of the recycling of CCA-impregnated wood. In the first recycling of impregnated timber project the amount and location of recyclable treated wood in Finland was determined and the costs of recycling were calculated. An incineration test was also made (1998). The conclusion of the first project i...
T Syrjänen, E Kangas


Nouvelles techniques de lutte anti-termites à faible impact environnemental
1995 - IRG/WP 95-50040-32
P Martinet


What can DNA fingerprinting, aggression tests and morphometry contribute to the identification of colonies of the Formosan subterranean termite
2000 - IRG/WP 00-10371
Multilocus DNA fingerprinting, aggression tests and morphometry were compared to evaluate their potential for the identification of colonies of the Formosan subterranean termite, Coptotermes formosanus (Isoptera: Rhinotermitidae) in Hawaii. DNA fingerprinting separates the termites from all studied collection sites. Since the genetic similarity between termites from different collection sites lies...
C Husseneder, J K Grace


Detection of defects in standing poles by X-ray techniques
1980 - IRG/WP 2132
The application of X-ray techniques to wood pole inspection procedures has been evaluated. Internal defects were accurately identified using X-ray inspection methods. These methods and their possible influence on the safety and economic aspects of wood pale utilization are discussed....
W D Gardner, R S Johnstone, W Pitt


Baiting techniques for subterranean termite control
1983 - IRG/WP 1205
The use of artificial baits, with and without toxins, have proved effective in attracting large numbers of non-mound and mound building subterranean termites in both urban and rural areas. An outline is presented of a termite baiting program which emphasises concurrent laboratory and field investigations on termite ecology, behaviour and physiology...
J R J French, J P Robinson


Techniques for field assessment of particulate termite barriers
2000 - IRG/WP 00-10376
Field testing of particulate termite barriers poses problems different from those encountered when testing preservatives or soil termiticides. To ensure an adequate level of challenge, and minimise the risk of a Type II error, the experimental design must promote termite activity and provide a significant, readily detectable, food source which can only be reached by penetrating the test barrier. I...
D M Ewart, E R Rawlinson


Development of novel techniques for evaluating the feeding rate of Limnoria lignorum with specific reference to temperature influences
1999 - IRG/WP 99-10325
The faecal pellet production of Limnoria lignorum placed into repli-dish chambers containing seawater and a small chip of untreated Corsican pine (Pinus nigra) was observed. The repli-dishes were kept at a range of constant temperatures between 10 and 25°C and the number of faecal pellets produced by each of the animals was monitored. It was found that faecal pellet length generally increased wit...
A Praël, S M Cragg, R A Eaton


Molecular studies on isolates of Serpula lacrymans
1989 - IRG/WP 1421
The major protein species present in detergent extracts of 14 different Serpula lacrymans isolates have been compared, by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with a standard strain, viz. Serpula lacrymans FPRL 12C. Following silver staining of SDS gels the major protein species identified in 12 isolates were similar to those found in the standard strain. However ...
A Vigrow, D Button, J W Palfreyman, B King, B M Hegarty


Growth of two selected sapstain fungi and one mould on chitosan amended nutrient medium
2003 - IRG/WP 03-10466
In vitro studies were undertaken to investigate the effect of chitosan on growth of Leptographium procerum, Sphaeropsis sapinea and Trichoderma harzianum. Chitosan was tested at three molecular weight (MW) ranges and different concentrations formulated as either a powdered suspension or as a solution. The results generally showed that low MW chitosan produced a greater inhibitory effect on growt...
C Chittenden, R N Wakeling, B Kreber


Agenda Special Seminar: Screening techniques for potential wood preservative chemicals
1978 - IRG/WP 2115
IRG Secretariat


Developments in wood preservation processing techniques in New Zealand
1980 - IRG/WP 3143
P Vinden, A J McQuire


Molecular Microbiology and Wood Protection
2009 - IRG/WP 09-20416
During the last decade, the development of molecular tools has allowed significant advances in environmental microbiology. Such techniques have proved to be invaluable tools for the qualitative and quantitative description of environmental microbial communities. The aim of this paper, written for non-specialized readers, is to discuss the possible applications of such tools in wood preservation fi...
E Gelhaye, M Morel


Development of decay in untreated, second-growth Douglas-Fir using two exposure techniques in North Queensland
1997 - IRG/WP 97-20110
The results of two exposure techniques for evaluating the development of wood decay in untreated, mill-run lumber from second-growth Douglas-fir containing both sapwood and heartwood are presented. Nominal 50 mm by 100 mm by 2.5 m (2 in. by 4 in. by 8 ft) lumber, No 2 and better, was obtained from a production run in a mill that was processing second growth, Coastal Douglas-fir in western Oregon, ...
J Norton, S Kleinschmidt, R C De Groot, D Crawford


Isolation of the dry rot fungus, Serpula lacrymans, from the forests of the Himalayan Foothills
1995 - IRG/WP 95-10129
Previous expeditions to the Himalayas (Singh 1993, 1994) have reported on the presence in this area of the dry rot fungus Serpula lacrymans. However, attempts to isolate the organism from material brought back from these expeditions were not successful. In this paper we report on the isolation of Serpula lacrymans from spores and cord material brought back from the Narkanda region of the Himalayas...
N A White, J W Palfreyman, J Singh, S Singh


The effect of low molecular weight chelators on iron chelation and free radical generation as studied by ESR measurement
2000 - IRG/WP 00-10367
The focus of this work was to improve our current knowledge of the non-enzymatic mechanisms involved in brown-rot decay. Electron Spin Resonance (ESR), also known as Electron Paramagnetic Resonance (EPR), is an attractive technique for the identification and study of chemical species containing unpaired electrons (such as radicals and certain transition metal species). ESR spin-trapping techniques...
Yuhui Qian, B Goodell


A novel defaunation method of the protozoa to investigate cellulose metabolism in Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae)
1994 - IRG/WP 94-10050
The largest protozoa in the hindgut of workers of Formosan subterranean termite, Coptotermes formosanus Shiraki, was selectively eliminated by forced-feeding on low-molecular weight cellulose (LC) with a mean DP of 17. Although one week's feeding on LC caused perfect disappearance of Pseudotrichonympha grassii Koidzumi, the selective defaunation method itself had no detrimental effect on ...
T Yoshimura, K Tsunoda, M Takahashi


Summarized comparison of four techniques for detecting defects in poles
1979 - IRG/WP 2126
Since our discussions in open session of Working Group II at Peebles, a number of workers in Australia have collaborated in comparing the efficacy of X-ray, sonic testing, or resistivity measurements for detecting internal defects in poles. The purpose of this circular is to draw to your attention some of the results, in summary form. No attempt has been made to compare techniques. More detailed a...
H Greaves


Study of the degradation of retified wood through ultrasonic and gravimetric techniques
1994 - IRG/WP 94-40030
One of the non-polluting processes that increase the natural durability of wood is a thermal treatment in an atmosphere poor in oxygen, producing what is normally denominated "retified wood". In this study the validity of a non-destructive technique (ultrasound) in order to determine the biodegradation suffered by wood across time has been studied. For this reason, the behavior of a wood species (...
D T De Troya, A M Navarrete


Molecular analysis of the basidiomycete Coniophora puteana
1992 - IRG/WP 92-1534
Sodium dodecylsulphate polyacrylamide gel electrophoresis and Western Blotting, using a polyclonal antiserum produced against a whole cell extract of Coniophora puteana, were used to analyse the major proteins and antigens of the wet rot organism Coniophora puteana. The macromolecule profiles of this organism were different from other members of the Coniophora genus and from a set of unrelated org...
H E McDowell, D Button, J W Palfreyman


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