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Protein extraction from wood decay fungus Postia placenta
2014 - IRG/WP 14-10827
Wood decay fungi (often distinguished as white rot and brown rot) belonging to the basidiomycetes, are common inhabitants of forest litter, where they play an important role in carbon cycling. Brown rot fungi are perhaps the most important organism involved in the degradation of wood products, and of considerable economic importance. Brown rotted wood loses strength very early because of the rapid...
A Pilgård, P Arnold, K Richter


Potentialities of protein borates as low-toxic, long-term wood preservatives - Preliminary trials
1999 - IRG/WP 99-30212
Boron compounds are efficient wood preservatives, as well as safe for the mammals and environmentally acceptable. Their natural solubility allows them to treat almost any wood species, but is also the cause of their high depletion from treated timber in outside exposure. In order to reduce this leachability, potentialities of proteinic polymer networks retaining boron within the wood have been inv...
M-F Thévenon, A Pizzi, J P Haluk


Molecular studies on isolates of Serpula lacrymans
1989 - IRG/WP 1421
The major protein species present in detergent extracts of 14 different Serpula lacrymans isolates have been compared, by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with a standard strain, viz. Serpula lacrymans FPRL 12C. Following silver staining of SDS gels the major protein species identified in 12 isolates were similar to those found in the standard strain. However ...
A Vigrow, D Button, J W Palfreyman, B King, B M Hegarty


CCA Chemistry
1983 - IRG/WP 3268
A Pizzi


Inhibition of wood decay and termite damage by calcium precipitation
1996 - IRG/WP 96-30111
Fungal decay of wood in service, especially brown rot, results in billions of dollars (US) of losses annually. Recent environmental restrictions, both U.S. and international, are limiting or eliminating the use of broad spectrum biocides for wood preservation, primarily due to problems with disposal. In order to design new, environmentally benign methods for control of wood decay fungi, it is esse...
F Green III, T A Kuster, L Ferge, T L Highley


The course of fixation of Cu-Cr-As wood preservatives
1972 - IRG/WP 307
Copper-chrome-arsenic (CCA) preservatives in contact with wood result in an instant extensive increase of pH, because of ion-exchange and adsorption reactions with the wood. During precipitation of the active elements the pH continuously increases but reaches a maximum, when all chrome is consumed. Some of the early reaction products are unstable and slowly convert via dissolution into stable comp...
S-E Dahlgren


Effect of light and ventilation condition on the rate of wood decay by the brown rot basidiomycete, Tyromyces palustris
1991 - IRG/WP 1517
Effect of light and the ventilation conditions of incubation jars on the wood decay by Tyromyces palustris (Berk. et Curt.) Murr. FFPRI 0507 was investigated. Under no irradiation of light, the ventilation conditions gave extensive effect on mass loss of the test pieces when the culturing was performed with culture medium designated in Japanese Industrial Standard (JIS) A 9302 (Medium A; glucose 4...
T Suzuki, M Higaki


Inhibition of termite damage by N'N-napthaloylhydroxyamine (NHA): Reticulotermes flavipes (Kollar) vs. Coptotermes formosanus Shiraki
2000 - IRG/WP 00-10354
The calcium precipitating agent NHA has been shown to protect southern yellow pine (SYP) from wood decay and termite damage comparable to CCA in field tests (Gulfport, MS) for two years (Crawford and Green, 1999). In a collaborative study, SYP wood blocks were vacuum treated with three concentrations of aqueous NHA and exposed in a no-choice test to Eastern subterranean termites (FPL, USA) and For...
F Green III, S T Lebow, T Yoshimura


Transformation of Ophiostoma picea and Trichoderma harzianum with green fluorescent protein (GFP)
2003 - IRG/WP 03-10477
While microbial colonization of wood is presumed to be characterized by a myriad of interactions between numerous organisms, studying these processes is often difficult owing to the opaque nature of the wood and the inability to readily distinguish among the many species colonizing the material. One method for enhancing the ability to distinguish organisms is to induce specific proteins in one or ...
Ying Xiao, L M Ciuffetti, J J Morrell


A comparison of the leaching resistance of diammine-copper complexes and copper carbonate precipitated in wood
1997 - IRG/WP 97-30158
Previous studies have shown that during treatment of wood with ammoniacal copper solutions, both simple copper precipitates and diammine-copper complexes are formed. The objective of the present study is to determine the relative importance of both forms of copper, on such aspects as preservative leachability and biological performance. In the current experiment, the leachability of copper carbona...
Xiao Jiang, J N R Ruddick


Kinetics and mechanism of fixation of Cu-Cr-As wood preservatives. Part 6: The length of the primary precipitation period
1975 - IRG/WP 359
The end of the primary precipitation fixation period of CCA preservatives coincides with the first peak in pH versus time. This offers a simple way of estimating the duration of the period. The duration is determined by a number of factors and their interactions, the most important of which are: wood species (anatomy, natural pH, accessibility of reducing agents), preservative type, preservative c...
S-E Dahlgren


Effect of Volatiles from Trichoderma species on the regulation of protein synthesis in Serpula lacrymans isolates
2002 - IRG/WP 02-10440
The growth of wood decay fungi has previously been shown to be inhibited by volatile organic compounds (VOCs) from selected Trichoderma isolates. No mechanism of action has however been established for such inhibition. This paper reports the effects of VOCs produced by Trichoderma pseudokoningii, T. viride and T. aureoviride on the growth of four dry rot isolates and corresponding protein synthesi...
S Humphris, R Wheatley, E Buultjens, A Bruce


Reduction of preservatives leaching from wood by the application of animal proteins
2005 - IRG/WP 05-30387
The investigations consisted in the estimation of the effectiveness of protein borates and protein propionates in wood preservation against Coniophora puteana. The estimation was made on the basis of mass losses caused by the test fungus action against treated wood which was subjected to leaching with water. The investigations were performed for Scots pine wood (Pinus sylvestris L.) treated with p...
B Mazela, I Ratajczak, M Bartkowiak


Effects of Prior Establishment of Trichoderma harzianum on Ophiostoma picea Growth in Freshly Sawn Douglas-fir Sapwood
2003 - IRG/WP 03-10476
Trichoderma harzianum has been shown to be an effective biocontrol agent against a number of wood inhabiting fungi under laboratory conditions, but this fungus has performed poorly in field trials. Understanding the interactions between biocontrol agents and their intended targets in wood may provide important clues for developing improved approaches to biocontrol, potentially reducing our relianc...
Ying Xiao, J J Morrell, L M Ciuffetti


Effect of post-treatment processing on leachability of ACZA-treated douglas-fir lumber
1998 - IRG/WP 98-50109
The effect of post-treatment procedures that more rapidly precipitate copper, zinc, and arsenic in douglas-fir treated with ammoniacal copper zinc arsenate (ACZA) on subsequent leaching resistance were investigated at two retentions (6.4 and 40 kg/m3). Total leaching was greater from boards treated to the higher retention. At both levels, copper was lost at the highest rate. Post-treatment process...
J J Morrell, C S Love, S Kumar, C M Freitag


Animal blood protein as a component of new, non-toxic wood preservatives fixing organic active compounds in wood
2003 - IRG/WP 03-30312
Application of natural organic compounds and simple physico-chemical treatments in wood conservation has, as its objective, not only its protection against biological agents but, equally importantly, protection of natural environment by gradual abandonment of toxic constituents still applied commonly on a wide scale. The introduction into wood of new, non-toxic impregnation agents manufactured on ...
B Mazela, I Polus


Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley


Characterization and differentiation of wood rotting fungi by protein and enzyme patterns
1999 - IRG/WP 99-20177
Standardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several auth...
U Schoknecht


Characterization of protein patterns from decayed wood of loblolly pine (Pinus taeda L.) by proteomic analysis
2008 - IRG/WP 08-10654
The primary biotic decomposers of wood belong to the basidiomycetes. The members of this group can attack and biodegrade both wood in the forest and in service. By the time wood decay is visible, there has already been a significant loss of strength. The identification of basidiomycetes and other organisms on wood only tell us what is present, not what is actively decaying the wood. When organisms...
Young-Min Kang, L Prewitt, S Diehl


Structural changes, basidiomycete richness, enzyme activity and proteomic profiling of decay resistant and non-resistant woods over 18 months in soil contact
2010 - IRG/WP 10-10733
Wood decay fungi damage wood by production of enzymes that attack the structural components. The objective of this study was to better understand which suite of decay genes and proteins are expressed during biodeterioration of three different wood types in forest soil over time. Variation in decay genes and proteins were determined for pine (non-resistant), cedar (naturally durable), and ACQ-treat...
Youngmin Kang, S Diehl, L Prewitt, D Nicholas


Mineralization of European oak with various ionic salt solutions to achieve an in situ precipitation of calcium oxalate
2019 - IRG/WP 19-40861
Thin specimens of European oak (Quercus spp.) with the dimensions of 4 × 20 × 50 mm3 were treated with various aqueous ionic salt solutions of calcium chloride, potassium oxalate and calcium acetate. Additionally, the oak was treated with combinations of calcium chloride and potassium oxalate, as well as calcium acetate and potassium oxalate with the aim to precipitate in situ the water insolubl...
T Franke, T S Volkmer


Influence of weathering of wood surfaces before and after treatment with iron(II) sulphate
2023 - IRG/WP 23-30786
Outdoor wood is exposed to various factors that cause the wood to grey or age. The ageing processes can vary greatly depending on the exposure. Parts of the wood that are not exposed to external factors, e.g. under the eaves, are less exposed and the ageing process is therefore slower. This can be accelerated by solutions based on iron ions. In this way, the wood greys quickly and evenly. However,...
B Lesar, M Humar, F Osvald