Your search resulted in 249 documents. Displaying 25 entries per page.
Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA). The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth
Characterization of tropical wood-decaying fungi by RFLP analysis of PCR amplified rDNA
1998 - IRG/WP 98-10251
Five brown-rot fungi (Tyromyces palustris, Gloeophyllum trabeum, Antrodia sp., Poria placenta, Coniophora puteana) and three white-rot fungi (Coriolus versicolor, Pycnoporus sanguineus, Lentinus squarrosulus) registred as representative wood-decaying fungi for international standards of wood durability tests have been characterized using molecular tools (PCR/RFLP). The Internal Transcribed Spacer ...
A Zaremski, M Ducousso, Y Prin, D Fouquet
16S rRNA Analysis of the Bacteria Associated with Biocide Degradation
2004 - IRG/WP 04-10543
The bacterially mediated degradation of the new anti-fungal biocide, bethoxazin, was studied in vitro, by means of 16S rRNA PCR-amplification and cloning techniques. Woodblocks impregnated with a subtoxic concentration of bethoxazin were incubated in compost, and the micro-organisms associated with the wood after 6 and 12 weeks was studied. It was found that after 6 weeks the wood contained a la...
D F Wallace, D J Dickinson
Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP II: Results from field studies
2007 - IRG/WP 07-30429
The effects of Chlorothalonil (CTN) and Butylated Hydroxytoluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and the non-biocidal BHT was evaluated for its synergistic effects with CTN. ACQ-C was a positive control and untreated SYP sta...
G T Kirker, M L Prewitt, S V Diehl
Microbial Community Analysis Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis: Field Study Results
2008 - IRG/WP 08-20377
The effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on the fungal community in southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis. Field stakes treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1 and 0.25% CTN, and 0.1 and 0.25% CTN combined with 2% BHT were installed with untreated controls in field s...
G T Kirker, S V Diehl, M L Prewitt, W J Diehl
Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima
Bacterial Degradation of Pentachlorophenol Indicated in Groundwater Undergoing Long Term In-Situ Remediation
2016 - IRG/WP 16-50318
Pentachlorophenol (PCP, Penta) is a worldwide soil and water contaminant. The pervasive use of PCP as a fungicide, herbicide, pesticide, and as preservative for wood products has led to its global distribution. It is a highly effective preventative measure for most invasive insects, bacteria, fungi, and molds, as well as an excellent wood protection treatment. This study examined a known PCP conta...
C E Stokes, H Borazjani, L Prewitt
Analysis of tebuconazole in wood treated with Tanalith™ E
1999 - IRG/WP 99-20158
A simple gas chromatographic method for determining tebuconazole in Tanalith™ E treated wood is described. A two step sequential extraction procedure with methanol was used. Sample extracts were analysed without cleanup or concentration using capillary column GC with thermionic specific detection. The performance of the method was assessed using radiata pine (Pinus radiata) sapwood, radiata pine...
D E Ferlazzo
The applicability of life cyle analysis and alternative methods in the wood preservation industry
1994 - IRG/WP 94-50023
In the Netherlands, several case studies have been performed using the life cycle analysis method (LCA). This type of research is aimed at an inventory and classification (sometimes including also evaluation) of the environmental impacts of a product, from the raw material to waste stage ("cradle to grave" approach). In a LCA each environmental impact is assessed in terms of, for example, mass of ...
P Esser, J Cramer
Utilization of curcumin for detection of presence of boron in wood
1982 - IRG/WP 3191
It has been shown that curcumin is not a reliable reagent for detecting boron in wood that has been attacked by fungi...
Results of chemical analyses in the field of wood preservation in the Bundesanstalt für Materialprüfung
1973 - IRG/WP 321
The results of qualitative and quantitative chemical analyses of wood preservatives are often the basis for evaluating the various works in the field of wood preservation. In the past 10 to 15 years a number of such works was carried out in the Bundesanstalt fur Materialprüfung, Berlin-Dahlem, dealing with the identification and effectiveness of wood preservatives and with methods of wood preserv...
H J Petrowitz
Modelling of PCP migration in the environment: Feeding the models with laboratory data
1993 - IRG/WP 93-50001-08
In 1989, Hydro-Québec began a study program on pentachlorophenol (PCP) to ensure safe use of the product at all stages. One of the aspects of the study is the creation of a predictive system for evaluating the behavior of PCP and oil migration from wood poles to the environment. This system comprises four mathematical models for predicting PCP and oil migration in and on the surface of the pole, ...
A Besner, P Tétreault, R Gilbert
Penetration analysis of two common bamboo species - borak and jawa of Bangladesh
2002 - IRG/WP 02-40247
Preservative treatment of two bamboo species, namely borak (Bambusa balcooa Roxb.) and jawa (Bambusa salarkhanii Alam) was carried out with chromated copper boron (CCB) preservative by dipping method. The variation in preservative penetration between the two different species was determined. It was found that preservative penetrates into borak quicker than into jawa and easier into air-dried bambo...
M O Hannan, A K Lahiry, N M Islam
Finite element analysis of boron diffusion in wooden poles
2003 - IRG/WP 03-40263
The problem of describing the migration of dissolved boron in wood is treated with special reference to the commonly used remedial treatment of wooden poles. The governing equations are derived and discussed together with some of the material parameters required. The equations are solved by the finite element method and finally, results showing the effect of different treatment strategies are pres...
K Krabbenhøft, P Hoffmeyer, C G Bechgaard, L Damkilde
Detection of defects in standing poles by X-ray techniques
1980 - IRG/WP 2132
The application of X-ray techniques to wood pole inspection procedures has been evaluated. Internal defects were accurately identified using X-ray inspection methods. These methods and their possible influence on the safety and economic aspects of wood pale utilization are discussed....
W D Gardner, R S Johnstone, W Pitt
Conforming to european standards for preservative-treated timber: Specifying with confidence
2000 - IRG/WP 00-20194
A four-year collaborative study between four industrial partners and BRE has assessed timber treated by current UK industrial practices in the light of current European Standards. Data were collected for CCA and creosote treated timber components, and compared with the requirements laid out in EN351-1 and -2. A number of difficulties were encountered that have been described in previous IRG papers...
E D Suttie, R J Orsler
Quantitative determination of Chromium: A comparison of three instruments
1995 - IRG/WP 95-50053
A comparison is made between three instruments for measuring levels of chromium in the leachate of copper-chrome-arsenic (CCA) treated timber. These include an atomic absorption spectrophotometer, an inductively coupled plasma spectrometer and the RQflexâ. The RQflexâ is a new hand-held instrument which measures chromium by dipping a ReflectoquantÒ strip into the leachate and inserting the stri...
S Walley, P R S Cobham, P Vinden
Electron microscopic detection and chemical analysis of three-lamellar structures in wood-destroying fungi
1984 - IRG/WP 1240
In the course of transmission electron microscopical investigations of pine wood decay by various brown- and white-rot fungi extracellular three-lamellar structures (TLS) formed by the fungi were found in specimens stained with ruthenium red. These structures occured in the lumen of the wood cell surrounding the hypha at the outermost layer of the fungal cell wall. In the course of the investigati...
R Foisner, K Messner, H Stachelberger, M Röhr
Observations on the failure of anti-sapstain treated timber under non-drying conditions
1990 - IRG/WP 1437
A range of bacteria and yeasts were isolated from antisapstain treated timber and fresh sawdust. Solution samples containing 100 ppm of TCMTB in a nutrient medium were inoculated with these organisms and incubated at 25°C for 5 days. The levels of TCMTB remaining in solution were determined by HPLC analysis after this time. Results indicated high losses of active ingredient for a range of organis...
G R Williams
Collaborative field experiment: Analysis of copper and chromium in stakes of the four reference timbers
1982 - IRG/WP 3213
The stakes analysed belong to the Swedish set of the four reference timbers of the collaborative field experiment (Document No: IRG/WP/367). Before the field exposure 60 mm were cut off the end of each stake. This was done in order to be able to carry out chemical analyses as well as different types of testing. Some of the material has been used in softrot tests (Document No: IRG/WP/1151)....
Collaborative soft rot tests: 2nd interim report
1975 - IRG/WP 258
J G Savory, J K Carey
A bibliography of organic solvent-based wood preservatives
1973 - IRG/WP 322
X-ray analysis of selected anatomical structures in copper/chrome/arsenic treated wood
1973 - IRG/WP 320
Application of analytical electron microscopy to problems in wood preservation has been very limited. Indeed, less than ten workers appear to have published their results using the technique, and of these' only two papers deal with energy dispersion procedures in the scanning electron microscope; the others employ the more familiar wavelength dispersive methods of the electron probe....
Comparative studies on the distribution of lignin and CCA elements in birch using electron microscopic X-ray microanalysis
1987 - IRG/WP 1328
The microdistribution of metal preservatives in treated wood has received considerable research over the last two decades. Despite this, little effort seems to have been made to try and correlate the distribution of CCA elements with respect to naturally occurring wood cell wall components in wood. In the present preliminary study an attempt is made to relate the distribution of lignin in-situ wit...
G F Daniel, T Nilsson
Ultrastructural and TEM-EDAX studies on the degradation of CCA treated radiata pine by tunnelling bacteria
1985 - IRG/WP 1260
An ultrastructural study was carried out on bacterial attacked Pinus radiata stakes treated with a high level (24.7 kg/m³) of Tanalith NCA preservative. The purpose of the investigation was to determine whether the organism possessed intracellular and/or extracellular detoxification mechanisms in order to overcome the high levels of copper, chrome and arsenic present within the wood fibre walls. ...
G F Daniel, T Nilsson