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In situ testing the influence of melamine resins on the enzymatic activity of basidiomycetes
1999 - IRG/WP 99-30194
Waterbased methanol etherificated melamine formaldehyde resins have the potential to increase the resistance of impregnated wood against wood destroying fungi. The mechanism of the increased wood durability is not clear yet. In the present paper the possible interference of melamine resins with wood degrading enzymes of Coniophora puteana and Trametes versicolor was investigated as a possible cont...
D Lukowsky, F Büschelberger, O Schmidt


Enzyme immunoassay to detect Postia placenta in field tests: Comparison of plate ELISA with hydrophobic cloth and cotton dipstick
1991 - IRG/WP 2378
Standard indirect enzyme-linked immunosorbent assay in polystyrene 96-well plates was compared to hydrophobic polyester cloth and cotton dipstick for detection of wood-derived antigens from the brown-rot fungus Postia placenta. The ease of handling, larger surface area, and economics of the latter two adsorbents were surveyed for application as field tests for detection of early decay. At high ant...
C A Clausen


The role of toilet paper in studies of desert subterranean termites in Arizona, USA
2000 - IRG/WP 00-10375
Toilet paper rolls were used as a substrate for observing foraging activity of Heterotermes aureus (Snyder) and Gnathamitermes perplexus (Banks) in the Sonoran Desert of Arizona. Foraging was minimal during the winter months, increased in the spring, was high but erratic in the summer, and then was moderate again in the fall. H. aureus foraged within a temperature range of 7.6° to 47°C, G. perpl...
M I Haverty


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


Production of monoclonal antibodies to Serpula lacrymans and their application in immunodetection systems
1993 - IRG/WP 93-10004
Monoclonal antibodies were produced against both whole cell mycelial extracts and exo-antigen extracts of the causative organism of dry rot, Serpula lacrymans. The antibodies were tested for cross-reactivity against fifteen strains of Serpula lacrymans and nine other fungal species. These species represented either other wood decay fungi or non-decay fungi commonly co-isolated with Serpula lacryma...
H Glancy, J W Palfreyman


Characterization and differentiation of wood rotting fungi by protein and enzyme patterns
1999 - IRG/WP 99-20177
Standardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several auth...
U Schoknecht


Production of fungal protoplasts from selected wood-degrading fungi
1991 - IRG/WP 1500
Studies of hyphal morphology and the effects of various chemicals on growth are often difficult to perform on filamentous fungi because of the difficulty of observing the protoplasm through the rigid hyphae wall and because most activity occurs in a limited region near the hyphal tip. As an alternative, hyphae can be reacted with certain cell wall degrading enzymes to remove the cell wall to produ...
C Rui, J J Morrell


Chitin synthetase activity in cellular fractions of wood-decay fungi
1992 - IRG/WP 92-1524
This study was an attempt to explain the variability of in vivo sensitivity of wood decay fungi to the chitin synthetase (CS) inhibitor, polyoxin, by comparing CS distribution in cellular fractions. The five species studied were the brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, Fibuloporia vaillantii, and the white-rot fungi Schizophyllum commune and Ganoderma applanatum. The CS activi...
B R Johnson, S C Croan, B Illman


Extracellular laccase production in brown-rot fungus Coniophora puteana
2001 - IRG/WP 01-10391
Coniophora puteana, one of representing brown-rot fungus, was investigated in terms of extracellular ligninolytic enzyme production. Three fungal strains of C. puteana were inoculated in liquid culture medium with sawdust of pine and oak wood. Extracellular laccase production was observed in some strains of C. puteana in the culture medium with sawdust of oak wood. The highest activity occurred at...
Kwang-Ho Lee, Seung-Gon Wi, Yoon Soo Kim


Wood preservation in Uruguay
1987 - IRG/WP 3404
The purpose of this paper is a brief description of the current status of wood treatment in Uruguay. The forest resources of the country are summarized, the environment, their economic importance and future potential. The present wood treating facilities are described, together with the chemicals used and the standards generally accepted. Wood treated products used in the Uruguayan market are cite...
G Baillod


Production of monoclonal antibodies to fungal metabolites
1986 - IRG/WP 1306
The role of fungal extracellular enzymes in wood biodegradation is incompletely understood. Our lab is beginning a project utilizing monoclonal antibodies to characterize extracellular metabolites of the brown rot fungus Poria placenta Fr. (Cooke). Monoclonal antibody technology takes advantage of the ability of antibody secreting spleen cells from immunized mice to fuse in the presence of polyeth...
J Jellison, B Goodell


Influence of the nutritional elements on pigmentation and production of biomass of bluestain fungus Aureobasidium pullulans
1997 - IRG/WP 97-10198
The effect of the carbon source and the amount of the nitrogen on the melanization and the production of mycelial mass of bluestain fungus Aureobasidium pullulans was studied by using solid state cultivations. The carbon sources used varied from easily soluble sugars to structural polysaccharides existing in lignocellulosic material. The amount of melanin was evaluated by using partial purificatio...
A-C Ritschkoff, M Rättö, F Thomassin


Wood preservation in Italy
1985 - IRG/WP 3354
The report summarizes the history of wood preservation in Italy up to the present time. Information on the Italian climate and the main hazards to timber are dealt with and the amounts of timber grown and imported and exported tabulated. The main commodities treated are poles and sleepers. The amounts of preservative used have been estimated and details listed of the 10 pressure plants in the coun...
A Gambetta, E Orlandi, R Cockcroft


Use of the enzyme-linked immunoassay (ELISA) and immunocytochemistry to detect soft rot in preservative treated and untreated wood
1990 - IRG/WP 2347
A polyclonal antibody was raised against mycelial extracts from the soft rot fungus Phialophora mutabilis. In ELISA assays, the antibody showed strong reactions with its own antigen and strong to moderate cross reactions with 6 other Phialophora soft rot species. With the exception of Ceratocystis albida, the antibody gave only weak or negative reactions with 11 other mold, blue stain and rot fung...
G F Daniel, T Nilsson


Effects of two insect growth regulators (IRGs) on Microcerotermes diversus (Silvestri) (Isoptera: Termitidae) in Southwest Iran
2006 - IRGWP 06-10601
Microcerotermes diversus (Silvestri) is the most economically damaging termite species in structures in southwest, Iran. One safe control strategy that may reduce the subterranean termite damage in buildings is the use of IGRs in a suitable bait matrix. The effects of two juvenile hormone analogues, Admiral (Pyriproxyfen ) and Farox on M. diversus were evaluated over a range of concentrations ( 10...
B Habibpour, M S Mossadegh, G Henderson, S Moharramipour


Oxidative stress and lignin peroxidase production in Phanerochaete chrysosporium
2008 - IRG/WP 08-10655
In Phanerochaete chrysosporium liquid cultures, the induction of lignin peroxidases is directly related to accumulation of reactive oxygen species at the mitochondrial level. In this study, we demonstrate that the expression of the mitochondrial thiol-related antioxidant system is not directly coupled to the LiPs expression. When the antioxidant systems are not able to cope with ROS accumulation, ...
M Morel, L Diss, C Fourrey, M Chalot, M Droux, J P Jacquot, E Gelhaye


Chemical Analysis in Production Quality Control at Wood Treatment Plants
2008 - IRG/WP 08-20396
Analysis methods for quality control analysis in wood treatment plants have evolved with the changes in treatment preservative chemistries and analytical instrument technology. The basic hydrometer specific gravity measurements used for solution strength and classic wet chemistry methods for wood have given way to instrumental techniques such as X-ray fluorescence, automatic titrator, and HPLC. No...
P Walcheski, L Jin


Material properties of furfurylated wood for window production
2009 - IRG/WP 09-40480
Wood modification with furfuryl alcohol is an extensively investigated process and already produced commercially. Furfurylated wood is in the focus of a European project on its use for the production of high performance windows. Different wood species were treated with furfuryl alcohol and tested on water uptake, dimensional changes, leaching in water, resistance to fungal degradation, and ecotoxi...
A Treu, A Pilgård, S Puttmann, A Krause


Effect of Heavy Metals on the Expression of Manganese Dependent Peroxidases of Phanerochaete chrysosporium
2010 - IRG/WP 10-10722
This work is part of an ongoing investigation that studies the application of a solution rich in Zn and Mn, obtained from a recycling process, as a preservative for wood. The effects of Zn2+ and Mn2+ on the enzyme-expression level of manganese-dependent peroxidase (MnP) from Phanerochaete chrysosporium was studied by real-time PCR. The glyceraldehyde 3-phosphate dehydrogenase gene (gpd) was used a...
C Ibáñez, M Rabinovich, M Barraco, G Gecchetto, M Cerdeiras


Structural changes, basidiomycete richness, enzyme activity and proteomic profiling of decay resistant and non-resistant woods over 18 months in soil contact
2010 - IRG/WP 10-10733
Wood decay fungi damage wood by production of enzymes that attack the structural components. The objective of this study was to better understand which suite of decay genes and proteins are expressed during biodeterioration of three different wood types in forest soil over time. Variation in decay genes and proteins were determined for pine (non-resistant), cedar (naturally durable), and ACQ-treat...
Youngmin Kang, S Diehl, L Prewitt, D Nicholas


Measurements of rot fungal activity as a function of moisture content by isothermal calorimetry
2010 - IRG/WP 10-20428
Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents. The results clearly indicate that the heat production rate (a measure of respiration rate and activity) is moisture dependent. When the moisture content is decreased, less heat is produced, and when the moisture content is increased, more heat is produced. I...
L Wadsö, A Pilgård, G Alfredsen


Optimization of oxalic acid production for bioleaching of metal components from CCA-treated wood by an unknown Polyporales sp. KUC8959
2010 - IRG/WP 10-50266
A brown-rot fungus, an unknown Polyporales sp. KUC8959, has recently been identified and proven as a prominent fungal species for bioremediation of CCA-treated wood wastes in our Lab. The fungus produced a larger amount of oxalic acid than other fungi tested, and removed 90 % or more of chromium, copper and arsenic from CCA-treated wood sawdust through bioleaching process. The bioleaching process ...
Yong-Seok Choi, Min-Ji Kim, Jae-Jin Kim, Gyu-Hyeok Kim


Climate change and wood protection, increasing demand of long life wood products and decreasing production of treated wood
2010 - IRG/WP 10-50269
Several new topics related to wood protection sector has appeared in Japan, as the awareness of climate change becomes bigger and bigger. The “Basic act for housing (2006)” and the “Long-life housing promotion act (2009)” were enforced to form a safe and high quality housing stock and to form a long-service-life housing respectively. Despite of the favorable background for wood protection ...
K Yamamoto, H Kato


Measuring fungal cellulolytic enzyme activity in degrading wood
2011 - IRG/WP 11-10753
An enzyme assay which measures enzyme activity directly from wood will assist in the fundamental understanding of the enzyme components of the decay mechanism of rot fungi and any changes in the presence of wood protectants. At present, to measure fungal carbohydrate degrading enzyme activity in the presence of wood, two methods are used: either 1) the enzyme is measured in the growth media contai...
S M Duncan, J S Schilling


Implementation of a Quality Assurance System for thermally modified timber (TMT) by the Association of Central European TMT-Producers
2011 - IRG/WP 11-40558
Due to increasing market shares of thermally modified timber (TMT) in Europe, a working group of ten central European TMT manufacturers (TMT-WG) was founded in 2010 to cover public relations and marketing activities. Since the present level of product testing and property declaration was not satisfying, a “task group TMT-quality” was put into action in 2010 to develop a comprehensive quality a...
C R Welzbacher, W Scheiding


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