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Successive collections of Basidiospores from wood decay fungi (in vitro) show variation in germination levels on common media
1978 - IRG/WP 191
In the course of various preliminary experiments in which spore germination levels of 6 decay fungi on malt and water agar were recorded as controls, it was noted that one could not reliably obtain an expected level of spore germination for any particular fungus. Inconsistent 'control' spore germination levels of a fungus greatly complicates large scale experiments in which compa...
E L Schmidt, D W French


Étude in vitro de la colonisation et de la dégradation structural du bois de hêtre par Coriolus versicolor (L.) Quélet
1976 - IRG/WP 158
L'attaque du bois de Hêtre par Coriolus versicolor (L.) Quélet, agent de pourriture fibreuse blanche est étudiée après des périodes d'exposition à ce champignon variant de 15 jours à trois mois. L'observation des premiers stades, c'est à dire de la colonisation du bois par les filaments de champignon est faite en microscopie optique, puis au fur et à m...
D Dirol


Basidiosporogenesis by brown-rot basidiomycetes in vitro
1995 - IRG/WP 95-10126
Basidiospores of wood-rotting basidiomycetes are a primary sourse of infection causing wood biodeterioration, especially in wood above ground. Most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objective of this study was to demonstrate in vitro methods that promote carpogenesis and basidiosporogenesis by the brown-rot fungi Antrodia car...
S C Croan


In vitro studies on the effect of chitosan on mycelial growth and spore germination of decay fungi, moulds and staining fungi
2004 - IRG/WP 04-10507
The effect of solubilised, low molecular weight chitosan on established mycelial growth of a range of decay fungi, moulds and staining fungi was investigated using nutrient medium amended with different concentrations of chitosan that ranged from 0.1 to 0.4% weight per volume (%w/v). Also, spore germination of Trichoderma harzianum and Leptographium procerum was examined on chitosan amended nutrie...
C Chittenden, B Kreber, N McDowell, T Singh


Ibuprofen inhibits in vitro growth of brown-rot fungi
1996 - IRG/WP 96-10160
Ibuprofen, a phenyl propionic acid derivative (2-(4-isobutylphenyl)propionic acid) inhibited growth in vitro of 17 brown-rot fungi at a concentration of 100 µg/mL, while exhibiting no growth inhibition for 8 white-rot fungi at the same concentration. Propionic acid did not inhibit fungal growth in vitro. Morphological changes were noted in fungi that were able to grow in the presence of ibuprofen...
C A Clausen


In vitro sporulation of selected wood decay fungi
1978 - IRG/WP 190
Basidiospores produced axenically without laborious attention in the laboratory are useful in studies of wood decay initiation. Such spores presumably approach those collected from natural sporophores in size and germinability (Morton, 1964). Production of spores in vitro by inversion of cultures grown on 2% malt extract agar in deep glass dishes (100x80 mm²) has been the preferred method (Morton...
E L Schmidt, D W French


Étude in vitro de la colonisation et de la dégradation structurale du bois d'aubier de Pin sylvestre par la Mérule: Serpula lacrymans Schum. ex Fr. S. F. Gray
1979 - IRG/WP 198
The degradation of Scots pine sapwood cell walls by Serpula lacrymans, a brown rot fungus, is observed after various periods of exposure from two weeks to twelve weeks. The observation by microscopy shows that the hyphae of Serpula rapidly invade the wood tissues as cell wall degradation starts. That deterioration is not gradual, it is observed to be very irregular as well within the whole of the ...
D Dirol


The effect of different concentrations of polesaver rods on the survival of selected decay fungi in liquid culture
1998 - IRG/WP 98-30166
An in-vitro method was developed to determine the effect of solutions of Polesaver Rods on the survival of selected decay fungi. Polesaver Rods are a diffusing preservative treatment containing fluorine and boron designed for the internal remedial treatment of power poles. Two white-rot fungi, Perenniporia tephropora and Trametes versicolor, and two brown-rot fungi, Polyporus verecundus and Gloeo...
M A Powell, T Deldot, C McEvoy


Basisiospore production by Lentinus lepideus in vitro
1987 - IRG/WP 2276
Evaluation of fungicides active against the lower fungi by spore based bioassays is very common. Possibilities of using similar assays to evaluate preservatives against brown rot and white rot fungi, especially for use in above ground constructions, are limited by the lack of methods for production of basidiospores. Tested media containing glucose 10-30 g/l and ammonium tartrate concentrations 2-2...
J Bjurman


The effect of storage and subculturing on in vitro fruit body formation and spore production in Gloeophyllum sepiarium and Oligoporus placentus
2001 - IRG/WP 01-20232
Spores are widely assumed to be the main mode of infection of wood in conditions conducive to decay above ground. In order to supply spores on demand as an experimental material, fruiting body and basidiospore production in vitro by Gloeophyllum sepiarium and Oligoporus placentus have been examined. The optimum medium, time to spore production, and duration of fruiting as well as fruiting body sha...
S Choi, J N R Ruddick, P I Morris


Basidiospore germination threshold against borate in-vitro may vary between liquid and agar media
1992 - IRG/WP 92-2405
The concentration of disodium octaborate (BAE) which prevented mycelial growth of a common brown rot and 3 white rot fungi was compared to that required to prevent basidiospore germination on malt extract agar. Spore germination sensitivity was lower (50%) for only one of the white rot fungi. However, when spore germination thresholds were tested in malt extract-borate solutions in well slides (no...
E L Schmidt, Ya-Lih Lin


Comparison of bluestain fungi growing in vitro and in vivo
1996 - IRG/WP 96-10149
Both moulds and bluestain fungi cause serious economic losses for forestry and timber processing industries and much research is aimed at finding environmentally and economically acceptable methods of control. It is especially important to study the growth of these fungi in freshly cut wood, which has been unaltered by drying or sterilisation, and which therefore resembles the substratum they woul...
A Uzunovic, J F Webber, D J Dickinson


In vitro antifungal activity of chilli against wood degrading fungi
2006 - IRG/WP 06-10572
The efficacy of chilli juice and/or chilli extract oleoresin as antisapstain agents was evaluated against two common sapstain fungi, Sphaeropsis sapinea and Leptographium procerum. Possible synergy between chilli juice and Lactobacillus casei as antisapstain agents was also assessed. Both the chilli juice and the oleoresin showed moderate antifungal activity. No growth of the test fungi was obse...
T Singh, C Chittenden, D Vesentini


In vitro evaluation of an integrated approach using Trichoderma harzianum and chitosan for the control of sapstain
2008 - IRG/WP 08-10659
In vitro assays were undertaken to evaluate the control of two sapstain fungi, Leptographium procerum and Sphaeropsis sapinea by a combination of chitosan or chitosan oligomer (CODP-14) and a naturally mutated strain of Trichoderma harzianum. Spore germination and hyphal growth of the test fungi were assessed on media amended with chitosan or chitosan oligomer with and without T. harzianum using ...
C Chittenden, T Singh


In vitro fungicidal activity of Tunisian essences extracts against Coriolus versicolor and Coniophora puteana
2014 - IRG/WP 14-20544
Bark of six Tunisian species including cork oak (Quercus suber L.), eucalyptus camaldulensis, alder (Alnus glutinosa), Aleppo pine (Pinus halepensis), nut pine (Pinus pinea) and Chelfi pomegranates (Punica granatum) were tested against two rot-fungi: Coriolus Versicolor (for leafy trees) and Coniophora Puteana (for conifers) according to an adaptation of the European guidelines (NF EN 113, 1986). ...
L Lajnef, N Ayed, B Charrier


In Vitro Growth of the Basidiomycetes Physisporinus vitreus strains in various conditions in comparison with some white rot fungi
2022 - IRG/WP 22-10987
The paper has preliminary test results to select Pysisporinus vitreus fungal strains for further experiments in a large research project on copper tolerance of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to dete...
E E Soytürk, S N Kartal