Your search resulted in 30 documents. Displaying 25 entries per page.
Chelator production and cellobiose dehydrogenase activity of wood inhabiting fungi
2000 - IRG/WP 00-10363
Multiple components have been postulated to be involved in the Fenton-based biological degradation of wood by brown rot fungi. Chelators have been implicated in essential iron reduction steps. Cellobiose dehydrogenase activity and quinone reducing activity have also been implicated in brown rot biodegradation. When biochelator production by three white rot fungi, three brown rot fungi and three wo...
Weihong Qi, J Jellison
Effect of medium-term degradation of beech wood by erosive (Phanerochaete chrysosporium) and lignin-selective (Ceriporiopsis subvermispora) strains of white rot fungi on its selected physical properties
2004 - IRG/WP 04-40292
At the Faculty of Wood Sciences and Technology a fungal delignification of normal and tension beech wood by erosive and lignin-selective strains white-rot fungi has been studied. The pre-treatment of both kind of wood samples was accompanied by partial delignification and apparent changes of their physical properties influencing the polar liquids penetration....
R Solár, S Kurjatko, M Mamonová, J Hudec
The microbiological treatment of chlorophenolic preservative in spent utility poles
1993 - IRG/WP 93-50001-24
The number of pentachlorophenol (PCP) treated poles being removed from service is increasing, but disposal options for this material are limited. Biological Degradation of the preservatives in spent poles, before disposal or reuse, offers a promising technique. The reduction of PCP in treated timber, after inoculation with PCP-degrading bacterial species, Rhodococcus chlorophenolicus and Flavobact...
A McBain, F Cui, J N R Ruddick
Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola
1988 - IRG/WP 1343
By applying immunoelectronmicroscopic methods, lignin peroxidase of the white rot fungus Phanerochaete chrysosporium has been localized in the cytoplasm of hyphae, close to the plasmalemma and on the plasmalemma. Infiltration of wood specimen with culture filtrates, concentrated 300-fold, gave clear information on the penetration of the enzyme into the wood cell wall. Penetration was restricted to...
E Srebotnik, K Messner
Lignin degradation by wood-degrading fungi
1986 - IRG/WP 1310
The wood-degrading white-rot fungus Phanerochaete chrysosporium, has been the subject of intensive research in recent years and, based upon isolation of the extracelluar enzyme ligninase, major advances have now been made toward elucidating the mechanism by which this fungus degrades lignin. From these developments, a model emerges which could explain the process by which wood-degrading fungi in g...
P J Harvey, H E Schoemaker, J M Palmer
Physiologic response of Phanerochaete chrysosporium to exposure to triazole fungicides
1994 - IRG/WP 94-10066
Triazoles are increasingly important fungicides which are employed for a variety of applications included wood protection. Several recent studies suggest that white rot fungi are more tolerant of triazole compounds than other wood degrading fungi. Cultural studies using a white rot fungus, Phanerochaete chrysosporium, and 0.2 or 0.8 ppm of tebuconazole or propiconazole suggested that mycelial dry ...
J J Morrell, R K Velicheti
Remediation of pentachlorophenol- and creosote-contaminated soils using wood-degrading fungi
1994 - IRG/WP 94-50021
Microbiological treatment of hazardous wastes has generally been associated with the use of bacteria. During the past decade a significant body of evidence has accumulated that demonstrates that fungi, in particular white-rot fungi, have the ability to degrade a wide range of hazardous organic compounds (xenobiotics) and thus might also be useful for treatment of materials contaminated with these ...
R T Lamar, T K Kirk
Changed permeability and surface properties of hornbeam wood due to its degradation by erosive strain of Phanerochaete chrysosporium
2004 - IRG/WP 04-40290
Specimens of hornbeam wood were submitted to a medium-term degradations by erosive fungus Phanerochaete chrysosporium. Biodegradations of specimens resulted in their apparent structural alterations, changes in permeability and increased intensity of interactions with polar liquids. Modified physical properties of biodegraded wood may significantly influence its penetration with polar media and che...
R Solár, S Kurjatko, L Reinprecht, M Mamon, V Vacek
Changes in pore structure and cell wall volume in wood decayed by brown- and white-rot fungi
1991 - IRG/WP 1501
Sweetgum (Liquidambar styraciflua L.) wood blocks were decayed by Postia (=Poria) placenta or Phanerochaete chrysosporium in soil-block cultures. Decay was terminated at various weight losses, and the pore volumes available to probes of various molecular weight and diameter were determined by the solute exclusion technique (Stone, J.E. and A.M. Scallan. 1968. Cellulose Chem. Technol. 2, 343-358.)....
D S Flournoy
Identification of phanerosporic acid in birch degraded by Phanerochaete chrysosporium
2003 - IRG/WP 03-10496
Extracts of Phanerochaete chrysosporium cultures grown on birch or on a malt extract-peptone-glucose agar medium were analysed by HPLC. A major component from the two sources appears to be identical by HPLC and UV-visible spectrometry. The product isolated from agar-grown cultures was purified to apparent homogeneity and structure analysis by NMR indicates that the metabolite is the beta-resorc...
M D Mozuch, P J Kersten
Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substrates
2003 - IRG/WP 03-10475
D-Glucose metabolism is thought to be important during wood decay by fungi, not only for anabolic and catabolic purposes of central metabolism, but also as a potential source of peroxide required by extracellular peroxidases. There has been some confusion in the literature as to whether this peroxide-generating activity is of the glucose 1-oxidase or pyranose 2-oxidase (glucose 2-oxidase) type wi...
T H de Koker, M D Mozuch, P J Kersten
Evidence for the participation of the osmiophilic particles in the enzymatic demethylation of lignin
1984 - IRG/WP 1239
Transmission electron microscopic observations of the white-rot fungi Trametes hirsuta and Phanerochaete chrysosporium grown on a series of wood pulp with a graded lignin content and mycelium from the clarified centre of an agar-medium containing lignin (E1-effluent from the bleaching process) showed that osmiophilic particles are only induced by native lignin. Taking into account that the osmioph...
K Messner, H Stachelberger
Quantification of wood decay effects by HPLC analysis
1992 - IRG/WP 92-1576
The present work quantified the effects of the white rot basidiomycetes Coriolus versicolor and Phanaerochaete chrysosporium, and also those of the brown rot fungi Coniophora puteana and Lentinus lepideus, on Pinus patula and Eucalyptus grandis. Wood colonisation was quantified by Kjeldahl nitrogen determinations converted to biomass assays, and degradation was quantified by weight losses produced...
V Singh, M Tarin, G D Shelver, A A W Baecker
Secretion of ligninolytic enzymes by hyphal autolysis of the white rot fungus Phanerochaete chrysosporium
1991 - IRG/WP 1480
The secretion of ligninases by Phanerochaete chrysosporium was investigated with polyclonal antibodies, followed by immmunogold-silver staining and light microscopy. After growing fungal mycelium on nitrocellulose, extracellular ligninases were detected around old, plasmaless hyphae, but not at arthrospores, chlamydospores, or blastoconidia. Labeling of fungal hyphae on coverslip cultures was obse...
R Lackner, E Srebotnik, K Messner
Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
The manner by which lignin is degraded in-situ in natural substrates by white rot fungi still remains a controversial issue particularly the distribution and role(s) played by lignin degrading enzymes (i.e. manganese II peroxidase and lignin peroxidase). In the present study, use was made of anti-manganese II peroxidase and immunolabelling techniques in conjunction with transmission electron micro...
G F Daniel, B Pettersson, T Nilsson, J Volc
Improved preservative penetration of spruce after pre-treatment with selected fungi. II. Creosote treatment, analysis and strength testing
1998 - IRG/WP 98-40106
This paper describes the creosote treatment and analysis of logs pre-treated with selected fungal agents as a method of increasing the porosity of the wood prior to preservative treatment. The paper also reports the subsequent strength testing of the timber to evaluate the effects of the fungal pre-treatment on the structural integrity of the wood.
European spruce logs were pre-treated with eithe...
E J Tucker, A Bruce, H J Staines, B Rosner, K Messner
The influence of drying and chemical stress on the lignin degradation in aspen by Phanerochaete chrysosporium
1992 - IRG/WP 92-1573
Wood wafers of Populus tremuloides sapwood were incubated on common media with the white rot fungus Phanaerochaete chrysosporium (wild isolate) and subjected to cyclic drying or chemical agents (low levels of borate or trifluoperazine [a calmodulin antagonist]). After a mass loss of approximately 5.5%, wafers were dried and analyzed for acid-insoluble lignin. Those wafers containing chemical agent...
E L Schmidt, Y-L Lin
Improved preservative penetration of spruce after pre-treatment with selected fungi. I. Fungal pre-treatment of pole sections
1998 - IRG/WP 98-40117
European spruce logs were pre-treated with either Phanaerochete chrysosporium, Dichomitus squalens, Trichoderma viride and Trichoderma aureoviride for incubation periods of between 1-4 weeks prior to pressure treatment with creosote in a commercial treatment plant and analysed for strength and creosote uptake (see part II Tucker et al.). A method to pre-treat logs was optimised. The pole sections ...
B Rosner, K Messner, E J B Tucker, A Bruce
Cation analysis of wood degraded by white and brown rot fungi
1992 - IRG/WP 92-1552
Changes in the concentrations and composition of cations within wood colonized by the brown-rot fungi Postia placenta and Gloeophyllum trabeum and the white-rot fungi Trametes versicolor and Phanaerochaete chrysosporium were analyzed using plasma emission and atomic absorption spectrophotometry. In degraded poplar wood significant decreases in pH and electrical resistance readings were observed as...
J Jellison, K C Smith, W T Shortle
Characterization of wood decay enzymes by MALDI-MS for post-translational modification and gene identification
2002 - IRG/WP 02-10442
The recent sequencing of the Phanerochaete chrysosporium genome presents many opportunities, including the possibility of rapidly correlating specific wood decay proteins of the fungus with the corresponding gene sequences. Here we compare mass fragments of trypsin digests, determined by MALDI-MS (Matrix Assisted Laser Desorption Ionization-Mass Spectrometry), with predicted mass fragments derive...
T H de Koker, P J Kersten
Production of fungal protoplasts from selected wood-degrading fungi
1991 - IRG/WP 1500
Studies of hyphal morphology and the effects of various chemicals on growth are often difficult to perform on filamentous fungi because of the difficulty of observing the protoplasm through the rigid hyphae wall and because most activity occurs in a limited region near the hyphal tip. As an alternative, hyphae can be reacted with certain cell wall degrading enzymes to remove the cell wall to produ...
C Rui, J J Morrell
Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium
1988 - IRG/WP 1364
The degradation of lignin in native lignocellulosic substrates by white rot fungi is poorly understood. Biochemical studies have shown the involvement of a number extracellular ligninolytic enzymes released by white rot fungi which are capable of the oxidative conversion of DHP's (lignin model compounds) in vitro, but to date conclusive evidence for occurrence of these enzymes in wood und...
G F Daniel, T Nilsson, B Pettersson
Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki
Oxidative stress and lignin peroxidase production in Phanerochaete chrysosporium
2008 - IRG/WP 08-10655
In Phanerochaete chrysosporium liquid cultures, the induction of lignin peroxidases is directly related to accumulation of reactive oxygen species at the mitochondrial level. In this study, we demonstrate that the expression of the mitochondrial thiol-related antioxidant system is not directly coupled to the LiPs expression. When the antioxidant systems are not able to cope with ROS accumulation, ...
M Morel, L Diss, C Fourrey, M Chalot, M Droux, J P Jacquot, E Gelhaye
Effect of Heavy Metals on the Expression of Manganese Dependent Peroxidases of Phanerochaete chrysosporium
2010 - IRG/WP 10-10722
This work is part of an ongoing investigation that studies the application of a solution rich in Zn and Mn, obtained from a recycling process, as a preservative for wood. The effects of Zn2+ and Mn2+ on the enzyme-expression level of manganese-dependent peroxidase (MnP) from Phanerochaete chrysosporium was studied by real-time PCR. The glyceraldehyde 3-phosphate dehydrogenase gene (gpd) was used a...
C Ibáñez, M Rabinovich, M Barraco, G Gecchetto, M Cerdeiras