Your search resulted in 81 documents. Displaying 25 entries per page.
Pyrolysis GC-MS as a tool for lignin analysis
1987 - IRG/WP 2201
Pyrolysis-GC-MS is a rapid process for analysis of rather complex organic compounds. The method has great application to petroleum shale oil and wood lignin. Small powdered samples (several mg) are placed in silica capillary tubes and rapidly heated in an inert atmosphere such as He or N2. Breakdown products of the pyrolysis are then trapped and held for an allocated time in an injector attached t...
L Doimo
NIR spectroscopy for rapid estimation of decay resistance
2004 - IRG/WP 04-20294
In Scandinavia Scots pine (Pinus sylvestris L.) is an important commercial tree species. Its heartwood has traditionally been used in constructions exposed to risk of decay. In a laboratory experiment Scots pine wood specimens sampled from inner heartwood, outer heartwood and sapwood were exposed to the brown rot fungus Poria placenta. In general outer heartwood was more resistant than inner heart...
P O Flæte, E Ystrøm Haartveit
Rapid loss of lignin at wood surfaces during natural weathering
1992 - IRG/WP 92-2390
Thin veneers of radiata pine (Pinus radiata D. Don) were exposed to natural weathering over a period of 30 days. Incident UV radiation was monitored at the test site. FTIR internal reflectance spectroscopy was used to study the chemical changes taking place at the veneer surface during weathering. Spectra obtained show that after only 3 days exposure there was substantial delignification at veneer...
P D Evans, K J Schmalzl, A J Michell
Isolation and identification of the trail pheromone of the subterranean termite Reticulitermes speratus (Kolbe) (Isoptera: Rhinotermitidae)
1990 - IRG/WP 1459
The trail pheromone was successfully isolated from whole body extract of the termite Reticulitermes speratus (Kolbe) through various chromatographic reactions. Its trail-following activity was examined by bioassays. The primary structure of the pheromone was determined as normal chain dodecatrien-1-ol by capillary GC-MS analysis. Moreover, by means of capillary GC-MS and capillary GC-FTIR analyses...
M Tokoro, M Takahashi, R Yamaoka
Über Hausbock Hylotrupes bajulus (L.), (Col., Cerambycidae), in Laubholz: Versuche mit Laubholzarten, Prüfung der Wirkung von Ligninstoffen und von Ginkgo biloba, Beobachtungen an Hesperophanes. [On House-longhorn Beetle Hylotrupes bajulus (L.), (Col., Cerambycidae) in hardwood: Investigations with several hardwood-species, tests on activity of lignin components and Ginkgo biloba, observations on Hesperophanes]
1982 - IRG/WP 1144
Hylotrupes bajulus-larvae feed and develop in softwoods, hardwood acts as a feeding poison. The reason for this is still little known. According to literature some observations contradict this established opinion and the question was raised, whether the colour-strain scutifer (= lividus) is able to live in hardwood. Testing ten European and tropical hardwood-species (following the DIN-EN test proc...
S Cymorek
Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing
2004 - IRG/WP 04-10512
Fungi selected in test fields in Germany and Estonia and fungi (Coniophora puteana) cultivated on medium have been identified by the molecular methods PCR (Polymerase Chain Reaction), T-RFLP (Terminal Restricted Fragment Length Polymorphism) and sequenced to species level. The samples from the German field test showed that the fruit body sample was a different fungus than from the mycelium sample....
U Råberg, N Högberg, C J Land
Old and new facts on the dry rot fungus Serpula lacrymans
1991 - IRG/WP 1470
The article collates some of the recent literature on the biology of the dry rot fungus Serpula lacrymans. The fungus can grow at 28°C, and maximum wood moisture is above 55%. Serpula Iacrymans degrades crystalline cellulose. The intensive production of extracellular oxalic acid is neutralized by calcium and iron. There is considerable variation among the strains with regard to factors such as gr...
O Schmidt, U Moreth-Kebernik
The identification of organic compounds in wood using thermal desorption GC-MS - possibilities and limitations
2001 - IRG/WP 01-20224
There is a clear need for the analytical characterisation and identification of organic compounds and their derivatives in different phases especially in timber. In this context the paper describes a rapid and powerful gas chromatographic method for the determination of insecticides, biocides and "other" organic substances in treated and/or modified wood. The main advantage of this procedure is th...
P Jüngel, E Melcher
Soft-rot in Tabebuia sp. wood used in water cooling tower:
identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius
Effect of origin and orientation of Radiata pine substrates on the development of fungal degrade
2000 - IRG/WP 00-30221
The objective of this study was to determine the influence of different radiata pine substrates on colonisation by sapstain fungi. Freshly-cut branch wood, stem wood and sawn lumber were used to prepare test samples with the largest wood surface area showing a transverse, radial or tangential face. Test samples were evaluated against sapstain fungi using a rapid laboratory antisapstain screening m...
B Kreber, D R Eden, C M Chittenden, B Carpenter, J G Van der Waals
Rapid fixation of Chromated Copper Arsenate (CCA) wood preservatives by microwave treatment
2000 - IRG/WP 00-40184
Rapid microwave heating of freshly chromated copper arsenate (CCA) treated timber indicates that rapid preservative fixation is possible within approximately 40 seconds. The leaching of CCA was evaluated using simulated rainfall. Cost analyses indicates that microwave fixation using an on-line conveyor belt fixation process with an output of 4m3/hour using a microwave power supply of 230 kW costs ...
G Torgovnikov, P Vinden, E Mapanda, P R S Cobham
Isolation and identification of non-decay fungi affecting the performance of alkylammonium compounds
1993 - IRG/WP 93-10020
The isolation of DDAC tolerant fungi was carried out on lumber treated with a range of antisapstain chemicals containing DDAC as one of their active ingredients. The tolerant fungi were selected by using malt media spiked with DDAC (100 and 2500 ppm). Isolations were tentatively identified into a range of fungi commonly found associated with wood deterioration (i.e. Penicillium sp. and Trichoderma...
A K Doyle, J N R Ruddick
Development of a mini-block test method for the rapid evaluation of preservative performance against Basidiomycte fungi
1991 - IRG/WP 2379
Screening procedures for new biocides used as wood preservatives can be a time consuming process using conventional Basidiomycete assay procedures. This is due mainly to the long exposure periods required to achieve adequate levels of decay (weight loss) in the test blocks. A number of alternative methods have been developed but most utilise artificial substrates such as filter paper or agar in wh...
J Brown, S Caswell, G R Williams
The potential application of rapid gas-chromatographic assay of microbial respiration to the monitoring of wood decay in field trial situations
1983 - IRG/WP 2196
Gas chromatographic detection of microbial activity (C02 production) within stakes in a field trial situation would appear to provide a sensitive, non-destructive and relatively rapid method for the quantitative assessment of preservative treatments. Most consistent results were obtained when stakes were removed from the soil, washed, saturated with water and incubated in sealed PVC tubes at 25°C...
M A Line
Method of embedding and staining of wood after biological testing to support the identification of decay type
1998 - IRG/WP 98-20131
A method for preparing wood for microscopic analysis after decay testing is described, with special regard to a novel fast simultaneous staining, which helps to identify different types of decay. The novel staining process is explained as well as the embedding of heavily decayed samples in polyethylene glycol (PEG) and picking up of sections by transparent adhesive tape. Special feature of the int...
A O Rapp
Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt
Isolation and identification of the fungal flora in treated wood. Revised technique
1977 - IRG/WP 159
At the 8th Annual Meeting in Wildhaus a paper was presented for discussion on the isolation of fungi from treated wood.·Since then work of this nature has been undertaken at Imperial College and as a result a revision of that document has been made and is presented here. The main alterations are: 1) To streamline the isolation procedure 2) Modification of the benomyl agar 3) The inclusion of a st...
C P Clubbe, J F Levy
Characterization of wood decay enzymes by MALDI-MS for post-translational modification and gene identification
2002 - IRG/WP 02-10442
The recent sequencing of the Phanerochaete chrysosporium genome presents many opportunities, including the possibility of rapidly correlating specific wood decay proteins of the fungus with the corresponding gene sequences. Here we compare mass fragments of trypsin digests, determined by MALDI-MS (Matrix Assisted Laser Desorption Ionization-Mass Spectrometry), with predicted mass fragments derive...
T H de Koker, P J Kersten
A novel method for evaluating the performance of water repellents
1987 - IRG/WP 2279
Application of water repellent formulations to timber surfaces offers protection from the weather, reducing degrade and movement. A novel test method has been developed to evaluate the comparative efficacy of such formulations when samples are exposed to weather. The method uses sapstain free sapwood of defect free, untreated, seasoned Radiata pine, (Pinus radiata D. Don.) Water Repellency was det...
A R Moffat
A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl
The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari
Identification and inhibitory effect of volatiles from different ages of a Trichoderma aureoviride culture on selected wood decay fungi.
1995 - IRG/WP 95-10110
The ability of a Trichoderma sp. to produce volatile organic compounds (VOCs) over a four week period of growth was examined and the inhibitory effect of these volatiles against four selected basidiomycetes over the same period was assessed. After trapping, on tubes filled with chromatography packing material, VOCs were analysed on an integrated automated thermal desorbtion mass spectrometer syste...
A Bruce, A Kundzewicz, R E Wheatley
Evaluation of rapid methods for detecting wood preservatives in waste wood
1994 - IRG/WP 94-50024
For the disposal of wood waste under ecological sound conditions information about its hazardous potential is required. Several analytical methods are available for the detection of most elements in wood preservatives. Industrial process conditions, however, demand methods with very short turn around times. In this research several methods of analysis were judged on their speed and accuracy. A num...
W J Homan, H Militz
The “wire-loop slicing technique” for the rapid field collection of large numbers of Coptotermes acinaciformis termites from above-ground mound colonies
2006 - IRG/WP 06-10583
This technique of rapidly slicing through an above-ground C. acinaciformis mound colony with a steel wire-loop attached to a vehicle allows the collection of large numbers of termites. The sliced mound topples onto a tarpaulin arranged on the ground, the outer wall layers break away in large segments, and the carton materials incorporating the termites are readily collected and transferred to larg...
B M Ahmed, J R J French
Identification of fungi colonising coated and modified wood exposed outdoors using sequencing and T-RFLP profiling
2006 - IRG/WP 06-20326
Wood decay and staining fungi are known to colonize coated and modified wood. Rapid and reliable ways of identifying fungi on coated and modified wood may improve diagnostics and product development. In this work nine fungi growing in painted wood panels exposed outdoors at a test field in Uppsala, Sweden, were identified using sequencing and T-RFLP (Terminal Restriction Fragment Length Polymorphi...
U Råberg, J Bijelovic, C J Land, S Bardage, N Terziev