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Estimation of mycelial biomass by determination of the ergosterol content of wood decayed by Coniophora puteana and Fomes fomentarius
1989 - IRG/WP 1415
The mycelial biomass of fungi decomposing wood materials may be estimated by the use of an ergosterol assay technique.ln decay tests, essentially according to EN 113, estimates made by HPLC analyses on wood decayed by Coniophora puteana and Fomes fomentarius show the increase in biomass in the wood blocks. The ergosterol contents were correlated with dry weight loss determinations. Degradation of ...
K Nilsson, J Bjurman


The dry rot fungus Serpula lacrymans. Examples of attack and remedial treatment
1988 - IRG/WP 1347
The film deals with several aspects of dry rot attack and eradication in buildings. The detailed biology and morphological charasteristics of the fungus are portrayed. The various forms of mycelial growth, the role of the strands in the nourishment and spread of the fungus, as well as the many types of fruitbody formation are outlined. Environmental and nutritional requirements of the fungus as we...
G Buchwald, B M Hegarty, W Metzner, R Pospischil, H Siegmund, P Grabow


Effects of some essential oils on wood destroying organisms
1993 - IRG/WP 93-10047
Three wood destroying fungi: Botryodiplodia theobromae Pat. (stain), Gloeophyllum sepiarium (brown rot), and Pycnoporus sanguineus (white rot) were exposed to six plant essential oils: the peppermint, kaffir lime or leech lime, lavender, tarragon, holy basil, and the eucalyptus. The peppermint oil showed most effective to inhibit fungal growth, while eucalypus oil was the least effective. The othe...
K Atisongkroh, C Anantachoke, P Lekuthai, S Pensook, T Kittirattrakarn


In vitro studies on the effect of chitosan on mycelial growth and spore germination of decay fungi, moulds and staining fungi
2004 - IRG/WP 04-10507
The effect of solubilised, low molecular weight chitosan on established mycelial growth of a range of decay fungi, moulds and staining fungi was investigated using nutrient medium amended with different concentrations of chitosan that ranged from 0.1 to 0.4% weight per volume (%w/v). Also, spore germination of Trichoderma harzianum and Leptographium procerum was examined on chitosan amended nutrie...
C Chittenden, B Kreber, N McDowell, T Singh


Effects of terpene compounds on the growth and peroxidase activity of Phellinus pini
1979 - IRG/WP 2130
The volatile compounds myrcene, limonene, a-pinene, a-terpineol, D-fenchol and 1,8-cineole, present in the oleoresin fraction of coniferous wood, were tested individually and as mixtures for their effect on the growth and peroxidase activity of cultures of six strains of Phellinus pini (Fr.) A.Ames and one strain of Phellinus chrysoloma (Fr.) Donk (Phellinus pini var. abietis (Karst.) Pilát). Phe...
L Väisälä


Theoretical and practical experiments with eradication of the dry rot fungus by means of microwaves
1992 - IRG/WP 92-1577
Engineer Claus Andersen constructed a device in 1986 for microwave treatment of fungal infested timber. The device was tested on ampullae with live fungal mycelium of the dry rot fungus. A 10 minutes treatment at 37°C gave satisfactory eradicating effect. The method has since been used in practice in approximately 100 instances. A spot-test control has shown satisfactory results....
J Bech-Andersen, C Andersen


Analysis of volatile emissions as an aid in the diagnosis of dry rot
1992 - IRG/WP 92-2393
The dry rot fungus, Serpula lacrymans was grown in pure culture on malt extract and on sapwood of pine. The volatile compounds emitted from the cultures were determined by diffusion sampling on tubes filled with Tenax TA, thermal desorption and gas-chromatography-mass-spectrometry in order to find markers for attack of the fungus....
J Bjurman, J Kristensson


The influence of gaseous oxygen concentration on fungal growth rates, biomass production and wood decay
1998 - IRG/WP 98-10283
The effects of air and several levels of oxygen balanced with nitrogen (% oxygen (v/v) nitrogen to 100%) on growth rates, biomass production and wood decay were investigated. The best technique for measuring daily growth rates in anaerobic jars was found to be by using 40 mm petri dishes which were attached to the walls of the jars. At the end of the test period the same petri dishes were also use...
S M Kazemi, D J Dickinson, R J Murphy


Quantification of wood decay effects by HPLC analysis
1992 - IRG/WP 92-1576
The present work quantified the effects of the white rot basidiomycetes Coriolus versicolor and Phanaerochaete chrysosporium, and also those of the brown rot fungi Coniophora puteana and Lentinus lepideus, on Pinus patula and Eucalyptus grandis. Wood colonisation was quantified by Kjeldahl nitrogen determinations converted to biomass assays, and degradation was quantified by weight losses produced...
V Singh, M Tarin, G D Shelver, A A W Baecker


An attempt to grow dry rot fungus Serpula lacrymans on mortar substrate in non-sterile conditions
1989 - IRG/WP 1385
According to many experts, Serpula lacrymans is probably the most dangerous wood destroying fungus in houses. Investigations of KOCH, A.P. (2) showed that incidence of this fungus in percent was around 20% when the frequency of some other standard fungi was far below (Coriolus versicolor: 0.2% for example). The work presented here is a part of a program carried out at CTFT for assessing the resist...
L N Trong


Estimates of wood-consumption rates by termites
1983 - IRG/WP 1201
Effects of 2 components; termite-biomass and experiment duration on estimates of wood-consumption rates (mg wood/g termite/day), were examined. Three models; (1) no mortality, (2) linear mortality and (3) nonlinear mortality were used to calculate mean standing-crop biomass of termites. Model (1) predicted a significantly lower wood-consumption rate than those based on models (2) and (3). No signi...
N-Y Su, J P La Fage


The effect of storage and subculturing on in vitro fruit body formation and spore production in Gloeophyllum sepiarium and Oligoporus placentus
2001 - IRG/WP 01-20232
Spores are widely assumed to be the main mode of infection of wood in conditions conducive to decay above ground. In order to supply spores on demand as an experimental material, fruiting body and basidiospore production in vitro by Gloeophyllum sepiarium and Oligoporus placentus have been examined. The optimum medium, time to spore production, and duration of fruiting as well as fruiting body sha...
S Choi, J N R Ruddick, P I Morris


Fouling organisms as indicators of the environmental impact of marine preservative-treated wood
1995 - IRG/WP 95-50063
This study evaluates the use of fouling organisms (epibiota) to assess the environmental impact of preservative treated wood. This paper presents initial findings from treated panels exposed for 6 months at Sagres, Algarve, Portugal. Panels were treated with CCA, two copper-containing quaternary ammonium (ACQ) formulations and creosote, with nominal retentions from 10 to 40 kg/m³ (creosote 25 pcf...
R M Albuquerque, S M Cragg


Extracellular mucilage (ECM) in wood decay basidiomycetes
2002 - IRG/WP 02-10439
The ability of wood decay basidiomycetes to produce extracellular mucilage (ECM) and its relationship with total biomass production is being investigated. Growth and ECM production by the brown-rot fungus Gloeophyllum trabeum (FPRL 108 N) and the white-rot fungus Coriolus versicolor (CTB 863 A) was assessed in liquid culture under different conditions and in the presence of the fungicide cyprocona...
D Vesentini, D J Dickinson, R J Murphy


Uptake of copper by mycelium of wood decay fungi growing on copper S-substituted thioglycolate containing nutrient media
1998 - IRG/WP 98-10291
Mycelia of Trametes versicolor, Coniophora puteana and Poria monticola were grown on potato dextrose agar (PDA) media, containing various concentrations of copper N,N-dimethyldithiocarbamoylacetate (which may be regarded as a copper S-substituted thioglycolate). The tested copper compound revealed relatively low fungicidal activity. After 13-18 day growing period, we determined concentrations of a...
M Humar, M Petric, F Pohleven, P Kalan


Effects of biocides on the extracellular mucilaginous material (ECMM) produced by two wood rotting basidiomycetes
2003 - IRG/WP 03-10469
Growth and production of extracellular mucilaginous material (ECMM) by the brown-rot fungus Gloeophyllum trabeum (FPRL 108 N) and the white-rot fungus Coriolus versicolor (CTB 863 A) was assessed in liquid culture, supplemented with the biocides CuSO4 and cyproconazole. The production of biomass in G. trabeum was significantly influenced by the concentration of CuSO4 in the medium. When CuSO4 was...
D Vesentini, D J Dickinson, R J Murphy


Why don't more people work with Serpula lacrymans nowadays? A discussion of some of the different approaches to experimenting with this unique fungus
1989 - IRG/WP 1383
Remarkably few laboratories are currently investigating this well-known fungus. Not only has it failed to gain acceptance as a standard test organism (for wood preservative evaluation) in many countries, but is also sometimes excluded from pure research studies when it ought to be an obvious first choice as a representative basidiomycete. This paper discusses some different approaches that have be...
J D Thornton


Influence of the nutritional elements on pigmentation and production of biomass of bluestain fungus Aureobasidium pullulans
1997 - IRG/WP 97-10198
The effect of the carbon source and the amount of the nitrogen on the melanization and the production of mycelial mass of bluestain fungus Aureobasidium pullulans was studied by using solid state cultivations. The carbon sources used varied from easily soluble sugars to structural polysaccharides existing in lignocellulosic material. The amount of melanin was evaluated by using partial purificatio...
A-C Ritschkoff, M Rättö, F Thomassin


The influence of pentachlorophenol on mycelial growth of wood decay fungi Trametes versicolor, Grifola frondosa, Hypoxylon fragiforme, and Coniophora puteana
2007 - IRG/WP 07-30437
Polychlorinated phenols have been one of the most commonly used biocides. In recent time they have been increasingly eliminated from the marked. Because of their high stability, they are still present in numerous products, including impregnated wood. In our research we studied in vitro the influences of pentachlorophenol and/or sodium pentachlorophenolate on mycelial growth of four wood degrading ...
F Pohleven, B Boh


A Review of Wood as Material and Source of Innumerable Products
2008 - IRG/WP 08-10662
As we known that “wood –which is a source of innumerable pruducts (Tsoumis, 1968)– is a fascinating and challenging material (Dinwoodie, 1975)”, and hence, “an understanding of the basic properties of wood and of the factors that affect its serviceability is vital background for the preservation of wood structures (Sherwood, 1986)”. This paper therefore presents an overview of structru...
I Usta, R Despot


Wood characterization of Tetraclinas articulata and evaluation of its resistance against lignilolytic fungi
2009 - IRG/WP 09-10697
Coniferous trees of the Tetraclinis articulata known under the Common name thuja from Berberie, is an endemic species from North Africa. The solid wood is much appreciated for its natural beauty and homogeneity and its quality for marquetry and furniture. The aim is to improve Tetraclinis articulate uses. At present, its wood is widely underestimated and sub-used compared to its announced qualitie...
F El Bouhtoury-Charrier, A Hakam, A Famiri, M Ziani, B Charrier


The Chelator Mediated Fenton System in the Brown Rot Fungi: Details of the Mechanism, and Reasons Why it has Been Ineffective as a Biomimetic Treatment in some Biomass Applications – a Review
2014 - IRG/WP 14-10828
The chelator-mediated Fenton (CMF) reaction requires the action of two types of chelating compounds. The first chelator, oxalate, solubilizes and then sequesters iron, and the second chelator reduces iron. Iron reduction must be controlled near the fungal hyphae to prevent damaging Fenton chemistry from occurring in that location. Similarly, iron reduction must be promoted within the wood/plant ce...
B S Goodell, M Nakamura, J Jellison


Effect of MVOC exposure on mycelial growth of wood rotting fungi
2021 - IRG/WP 21-10977
It is well known that wood rotting fungi produce microbial volatile organic compounds (MVOCs) as metabolites. In our previous studies, we have found that some MVOCs produced by wood rotting fungi are common to fungal species tested in the studies, while others are specific to each species. Furthermore, it has been also shown that each wood rotting fungi do not always produce the same type of MVOCs...
S Horikawa, R Konuma, M Yoshida


In Vitro Growth of the Basidiomycetes Physisporinus vitreus strains in various conditions in comparison with some white rot fungi
2022 - IRG/WP 22-10987
The paper has preliminary test results to select Pysisporinus vitreus fungal strains for further experiments in a large research project on copper tolerance of P. vitreus by chemotropic test, choice test, and agar plate toxicity tests and determination of the effect of various ions on oxalic acid secretion by these white rot fungi. In the project, it is also aimed to run fungal decay tests to dete...
E E Soytürk, S N Kartal


Impregnation of wood with antifungal compounds from low-quality tree biomass
2022 - IRG/WP 22-30763
In this paper we have reviewed recent research on the development of bio-based preservative formulations for wood done at the Department of wood science and technology, Ljubljana. Preservative formulations used in this investigation were prepared using plant polyphenols as biocidal agents. These nonstructural components of wood were stilbenes and flavonoids, and were extracted from wood of broken ...
V Vek, I Poljanšek, A Balzano, M Humar, P Oven


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