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Co-operative research project on L-joint testing. Sampling after 8 months exposure
1983 - IRG/WP 2208
It was anticipated in Document No IRG/WP/2192 that exposure of L-joints by the European co-operators would take place on 1 April 1983. Where L-joints were exposed at this time, sampling after 8 months exposure is due on 1 December 1983. The present document draws attention to relevant previous documents which describe the sampling methods to be adopted. It also provides Tables for recording the results.
J K Carey, A F Bravery


Update on lab and field test results for polymeric alkylphenol polysulfide treated wood
2002 - IRG/WP 02-40240
The possibility of using Polymeric Alkylphenol Polysulfide (PXTS) as a wood preservative has been undergoing evaluation in our laboratories for the past four years. Various formulations prepared from this compound have been tested and found to be effective against wood decay fungi and insects in field stakes after 42 months exposure. In accelerated soil bed tests, PXTS has been shown to be considerably more effective than creosote against both soft-rot and basidiomycete decay fungi. PXTS has also been shown to be effective against marine organisms and after 18 months exposure appears to have about the same efficacy as creosote. Preliminary treatment trials indicate that southern pine can be readily treated by the empty cell process
J Goswami, A Abramson, R Buff, D D Nicholas, T Schultz


Co-operative research project on L-joint testing. Progress report to March 1988
1988 - IRG/WP 2315
Further sets of data received from CTFT (France), BAM (Germany) and PRL (UK) after 46-48 months exposure and STU (Sweden) after 22 months exposure are presented and discussed in conjunction with data reported previously. Colonisation and attack of the L-joints has progressed with increasing exposure period. The new data are generally in agreement with those presented previously and the major difference between Institutes continues to be one of rate of colonisation rather than any relative difference in performance of the treatments. Overall 0.5% TnBTO 1 min dip treatment is providing least protection followed by 1.0% TnBTO 1 min dip treatment. The double vacuum treatments continue to provide better protection than the dip treatments; there are now indications that 0.5% TnBTO double vacuum treatment is less effective than 1.0% TnBTO.
J K Carey, A F Bravery


Short term preconditioning of preservative-treated wood in soil contact in relation to performance in field trials
2000 - IRG/WP 00-20185
The effect of pre-exposure to primary colonising micro-organisms on preservative-treated wood, prior to a basidiomycete decay test, was determined by preconditioning in two soil types. Scots pine EN 113 blocks treated with 3 model systems (a triazole, a copper quaternary compound and a copper boron triazole) were leached according to EN 84 and subjected to 6 weeks and 8 weeks burial in either John Innes no. 2 (a loam-based horticultural compost) or soil from the Simlångsdalen field site in Sweden. The samples were then tested according to the method described in EN 113. Selective isolations were also performed after soil exposure and compared with those from a longer term field trial. Preconditioning lowered the effectiveness of the 2 copper containing preservatives. Some effect of soil pre-exposure could be noted with the triazole but this was limited. The fungal isolations from preconditioned EN 113 blocks and field exposed stakes were a similar mixture of soft rot and mould fungi. Bacteria were commonly isolated from the preconditioned wood. The role of these micro-organisms in the modification of the preservatives is currently being investigated.
S Molnar, D J Dickinson


Natural durability and basic physical and mechanical properties of secondary commercially less accepted wood species from Brazil
2002 - IRG/WP 02-10451
The natural durability towards basidiomycete fungi and termites of several secondary timber from Brazil (Vatairea sp., Hymenolobium sp., Inga sp., Manilkara sp., Caryocar sp., Terminalia sp., Apuleia sp.) has been evaluated according to the EN 350-1. Some basic physical and mechanical properties (density, shrinkage, hardness, colour, static strength in flexion and compression) were also measured according to the appropriate standards. The results given in this paper could help in promoting the utilization of these timbers for certain end-uses.
M-F Thévenon, A Thibaut


Questionnaire for Volume 2 of the basidiomycete monographs
1985 - IRG/WP 1254
12 monographs of wood destroying basidiomycetes were published in volume 1. Volume 2 includes the following 17 basidiomycetes: Antrodia serialis, Chondrostereum purpureum, Climacocystic borealis, Fomitopsis pinicola, Hyphoderma tenue, Lentinus degener, Lentinus squarrulosus, Paxillus panuoides, Phellinus contiguus, Poria xantha, Pycnoporus sanguineus, Rigidoporus vitreus, Serpula himantoides, Sistotrema brinkmannii, Stereum sanguinolentum, Trametes corrugata
T Nilsson


Report on the status of collaborative experiments within the Sub-group on Basidiomycete tests
1983 - IRG/WP 2194
This report summarises the results of co-operative work carried out within the Sub-Group on Basidiomycete tests up to December 1982. The principle findings are recorded in the Conclusions Section. Work intended between IRG-13 in Turkey and IRG-14 in Australia is cited under Future Programme. An Annex provides a response sheet for existing and new participants to notify their contributions.
A F Bravery


Sub-group on Basidiomycete tests: Responses to proposals for programme of work
1980 - IRG/WP 2137
A F Bravery


The effect of storage or simulated internal use on the durability of wood based panels to decay fungi
1997 - IRG/WP 97-20106
Wood based panels such as particleboard and medium density fibreboard are experiencing a steady increase in use, in areas from structural to decorative applications. Understanding the "natural" durability of these panel products against basidiomycete fungal decay is therefore of great importance. Various methods have been proposed or are under development to test the durability/susceptibility of various panel products to decay. Our research has shown that if the fungal exposure methods detailed in the current European pre-standard (DD-ENV 12038:1996) are used to test boards fresh from manufacture, the susceptibility ratings observed are significantly lowered by the buildup of inhibitory substances in the test vessels. The effects of storing the boards before testing, in order to remove this effect have been studied with repeat biological tests carried out at intervals of 6 months. Our results show that even after 6 months storage the effect is reduced but not totally removed. The time after manufacture and the storage conditions are clearly significant variables affecting the "durability" of test specimens taken from the boards and therefore the incorporation of an appropriate preconditioning stage into the test is essential so that the effect is avoided.
S F Curling, R J Murphy, J K Carey


Sub-group on Basidiomycete tests: Proposals for Sub-group programme of work
1979 - IRG/WP 2127
During the IRG 10 Meetings in Peebles, Scotland in September 1978, it was resolved to establish within Working Group II a sub-group on testing wood preservatives against basidiomycete fungi. The terms of reference and scope of the sub-group's activities were to be determined by consensus among members who registered an interest. Dr A F Bravery (PRL, UK) was asked to act as co-ordinator. During the course of the Peebles Meeting the following members registered their intention to participate: Dr D Aston (UK), Dr C Coggins (UK), Mr G Fahlstrom (USA), Professor D French (USA), Professor C Jacquiot (France), Mr B Jensen (Denmark), Dr A Ofusu-Asiedu (Ghana), Miss J Taylor (UK). Since there was insufficient time to convene an inaugural meeting in Peebles the present paper has been prepared as a basis for discussions and to facilitate initiation of active collaboration. It is hoped that members will offer individual comment in order to define the desirable scope of the sub-group's work and to refine ideas for collaborative or co-ordinated experimental work.
A F Bravery


Practical testing of wood preservatives to prevent weathering damage and infection by micro-organisms on spruce and pine
1989 - IRG/WP 3530
Brush application, is generally used to prevent wooden window frames from decay caused by influence of weathering and fungi. Accordingly 10 boards of pine (Pinus sylvestris) and spruce (Picea abies) were treated with 10 commercially used wood preservatives. The efficiency of fungicides by using chiptest and blue stain test (EN 152), the effectiveness against weathering as well as the course of colonization of microfungi were measured on boards, that had been hurted by sawing. Results after one year's exposure showed that neither the normally required amount of 250 ml/m² nor the effective depth of penetration of the fungizidal equipment had been realized by using brush application. The smaller the permeability of the coating system, the more likely ideal conditions for the growth of fungi (e.g. basidiomycetes) are provided, answering hurtings of the coating and moisture contents of more than 30%. The variety of fungi (on pine 32 and on spruce 17 species) can be a result of anatomical differences and distinguished compounds of the wood species. After 24, respectively 36 weeks of exposure the frequent occurrance of basidiomycetous yeasts (Cryptococcus albidus, Rhodotorula mucilaginosa), blue stain fungi (Aureobasidium pullulans, Hormonema dematioides), Epicoccum nigrum, Alternaria alternata and Phoma species was noted.
R Gründlinger, O Janotta, H Melzer, K Messner


An experimental method to simulate incipient decay of wood by basidiomycete fungi
2000 - IRG/WP 00-20200
At very early stages of decay of wood by basidiomycete fungi, strength loss can be measured from wood before any measurable weight loss. Therefore, strength loss is a more efficient measure of incipient decay than weight loss. However, common standard decay tests (e.g. EN 113 or ASTM D2017) use weight loss as the measure of decay. A method was developed that allowed progressive removal of samples so that all stages of colonisation and decay could be monitored by strength testing, weight loss determination and chemical analysis. Our results indicated that substantial and rapid decay (90% strength loss and 40% weight loss after 12 weeks) of southern pine by brown rot fungi was possible using the method. Our results also demonstrate a direct relationship between strength loss and weight loss and suggest a quantitative relationship between strength loss and chemical composition (hemicellulose sugars) during incipient decay of southern pine by basidiomycete fungi.
S F Curling, J E Winandy, C A Clausen


Effect of light and ventilation condition on the rate of wood decay by the brown rot basidiomycete, Tyromyces palustris
1991 - IRG/WP 1517
Effect of light and the ventilation conditions of incubation jars on the wood decay by Tyromyces palustris (Berk. et Curt.) Murr. FFPRI 0507 was investigated. Under no irradiation of light, the ventilation conditions gave extensive effect on mass loss of the test pieces when the culturing was performed with culture medium designated in Japanese Industrial Standard (JIS) A 9302 (Medium A; glucose 4.0%, malt extract 1.5%, peptone 0.3%). On the other hand, no such kind of effect for ventilation conditions was recognized under light conditions, and the wood decay by the fungus accelerated by additional light irradiation of a considerably small intensity. Next, we investigated the relationship between light and the culture medium composition during the wood decay by the fungus. It was found that almost equivalent mass loss occurred after 60-90 days of cultures when the culturings were performed under light-shield conditions with medium A, the culture medium designated in Japan Wood Preserving Association (JWPA) standard No.1 (medium B; malt extract 2.0%, peptone 1.0%), and another culture medium diluted medium B by two times (medium C). Under the irradiation of light, the mass loss on the cultures in medium B and C was markedly less than that in the same media under no irradiation conditions. These results suggested that effect of light on the wood decay by Tyromyces palustris depended on the concentration of glucose in the culture medium. Further, we also investigated the activities of several extracellular and cell wall bound enzymes in wood meal medium contained medium A. From our experimental results, the activities of cellulase (b-1,4-glucan 4-glucanohydrolase, E.C. 3.2.1.4) and mannanase (b-1,4-mannan mannanohydrolase) depended on light irradiation during the wood decay and these enzyme activities may give extensive effect on the mass loss by Tyromyces palustris.
T Suzuki, M Higaki


Co-operative research project on L-joint testing. Progress report to March 1984
1984 - IRG/WP 2211
Each participant was intended to expose L-joints in the main trial on 1 April 1983 and the first sampling, after 8 months exposure, was to be undertaken on 1 December 1983. Some participants have had to vary this schedule. Results are presented from CTFT (France) and PRL (United Kingdom). These show a greater effectiveness by the double vacuum treatments compared with the 1 minute dip treatments. However, results from the two locations differ particularly in the incidence and type of Basidiomycetes isolated.
J K Carey, A F Bravery


Molecular analysis of the basidiomycete Coniophora puteana
1992 - IRG/WP 92-1534
Sodium dodecylsulphate polyacrylamide gel electrophoresis and Western Blotting, using a polyclonal antiserum produced against a whole cell extract of Coniophora puteana, were used to analyse the major proteins and antigens of the wet rot organism Coniophora puteana. The macromolecule profiles of this organism were different from other members of the Coniophora genus and from a set of unrelated organisms. However the profiles for Coniophora marmorata and Coniophora arida were more like that of Coniophora puteana than other organisms analysed. Analysis, by SDS-PAGE of exoproteins indicated differences between members of the Coniophora genus but, whilst there were some intra-species differences overall profiles were similar for all isolates of Coniophora puteana tested. Some cross reactivity of the Coniophora antiserum was noted, both in Western Blotting and in enzyme immunoassay and whilst the antiserum was produced against liquid culture grown organism it was able to detect Coniophora puteana when extracted from infected wood blocks. Furthermore, unlike some other basidiomycetes analysed serum components did not bind nonspecifically to Coniophora puteana.
H E McDowell, D Button, J W Palfreyman


Extracellular substance from the white rot basidiomycete Irpex lacteus involved in wood degradation
1992 - IRG/WP 92-1571
Cellulases, phenol oxidase, and a substance that both produced and reduced H2O2 were isolated from cultures containing wood or glucose on which the white-rot fungus Irpex lacteus was growing. The rate of wood degradation by the fungus with different amounts of glucose in the medium was measured. More of the substance that produced and reduced H2O2 was found extracellularly in cultures containing wood than in cultures containing glucose. The extent of one-electron oxidation activity of the extracellular substance was correlated with that of the wood-degrading activity, but the extent of phenol oxidase activity was not. The extracellular substance catalyzed the hydroxylation of phenol to hydroquinone and catechol in the presence of H2O2 or of NADH and O2. The substance seemed to be a glycopeptide containing Fe(II) and its molecular weight was very low.
H Tanaka, T Hirano, G Fuse, A Enoki


Co-operative research project on L-joint testing. Sampling after 18 months exposure
1984 - IRG/WP 2233
In September 1983, Document No: IRG/WP/2208 was distributed giving guidance on sampling after 8 months exposure (due 1 December 1983 for those L-joints exposed on schedule on 1 April 1983), and including tables on which to record the test results. No major problems have been notified to Princes Risborough Laboratory concerning the sampling method. It is therefore proposed that the next sampling, after 18 months exposure for those L-joints exposed on 1 April 1983, should proceed using the same methodology, on 1 October 1984.
J K Carey, A F Bravery


The high decay resistance in the sapwood of the naturally durable Malaysian hardwood Belian (Eusideroxylon zwageri)
2001 - IRG/WP 01-10410
It has long been assumed that the observed natural durability of the heartwood in certain timbers is perhaps associated with a relatively lower decay susceptibility also of the sapwood of these species. While the heartwood of Belian is reputedly highly decay resistant among the tropical hardwoods of Southeast Asia, laboratory decay tests reported in this paper have also confirmed the high decay resistance of the sapwood of this timber species. The sapwood of Belian is found to be resistant to decay by soft rot (Chaetomium globosum), white rot (Pycnoporus sanguineus & Coriolus versicolor) and brown rot (Poria sp. & Gloeophyllum trabeum) fungi after 12 weeks of decay testing, sustaining <2% mass loss, equivalent to that in its heartwood. The reference sapwood species Rubberwood (Hevea brasiliensis) and the heartwood of Kempas (Koompassia malaccensis) sustained significant mass losses from decay of up to 59% and 17%, respectively. Microscopic observations of Belian sapwood revealed extensive proliferation of extractives in the lumina of various cell types showing a pattern of extractive distribution similar extent to that in the heartwood tissues, although the extent of cell filling by extractives was not quite as high as for the heartwood. It is plausible that the inherent wood extractives deposited in the cells confer the same degree of anti-fungal properties to both the heartwood and sapwood of Belian.
A A H Wong, A P Singh


Co-operative research project on L-joint testing. Progress report to March 1986
1986 - IRG/WP 2272
Further sets of data received from CTFT (France) after 18 months exposure, BAM (Germany) after 18 months exposure, STU (Sweden) after 9.5 months exposure and TI (Denmark) after 12 and 17.5 months exposure are presented and discussed in conjunction with data previously reported. The new data are generally in agreement with those presented previously and indicate the major difference between the co-operating Institutes is one of the rate of colonisation rather than any relative difference in performance of the treatments. Overall 0.5% TnBTO 1 min dip treatment is providing least protection followed by 1.0% TnBTO 1 min dip. Both double vacuum treatments are providing better protection with little difference between 0.5% and 1.0% TnBTO.
J K Carey, A F Bravery


Co-operative research project on L-joint testing. Progress report to March 1985
1985 - IRG/WP 2236
It was originally intended that each participant would expose L-joints in the main trial on 1 April 1983 with the first sampling at 8 months (1 December 1983) and the second sampling at 18 months (1 October 1984). In the event, some participants have had to vary this schedule. Results after the first sampling have been received from CTFT (8 months), BAM (12 months) and Sipad-IRC (8 months) and after the second sampling from PRL (18 months) and Sipad-IRC (17 months). These, together with data presented previously, show a good correlation between gradual increases in permeability and the increasing incidence of Basidiomycetes. Both double vacuum treatments are proving more effective than the dip treatments; at the present stage 1.0% TnBTO appears to be no more effective than 0.5% TnBTO. The major difference between Institutes appears to be the rate at which colonisation and attack occurs rather than in the patterns of colonisation.
J K Carey, A F Bravery


The effect of soil pre-exposure on the results of laboratory Basidiomycete testing
1991 - IRG/WP 2385
Scots pine sapwood blocks were treated with several concentrations of copper chrome arsenic (CCA), copper chrome boron (CCB) and a copper modified quaternary ammonium compound (CMAAC). Leached and unleached samples were exposed in a basidiomycete monoculture test using Coniophora puteana, a copper tolerant brown rot. Prior to testing half of the blocks were buried in unsterile soil for 4 weeks. The soil pre-exposure had little effect on the performance of the CCA and CCB treated samples against the brown rot but the performance of the CMAAC treated samples improved greatly
S M Gray


Effects of the specimen position on fungal colonisation and wood decay by en 113 test fungi
1998 - IRG/WP 98-20136
For testing wood preservatives according to EN 113 it is common practice to plant the test blocks on neutral supports in order to prevent (1) a diffusion of chemicals into the agar medium and (2) an excessive moistening of the specimens. The procedure was employed in EN 350-1 for testing the natural durability of solid wood. It turned out to be of problematic nature because of the individual requirements of the test fungi. A certain degree of moisture can stop the virulence of one species, but - on the other hand - favour the virulence of another, so that different ratings of durability can be expected. In order to clarify this relation, blocks of durable and non-durable wood species were planted on different support materials and directly on the mycelial mat. Coniophora puteana, Coriolus versicolor, Gloeophyllum trabeum, and Poria placenta were used as test fungi. The results reveal a wide range with respect to the rate of colonisation and decay of the respective wood species if supports or no supports are used.
G Kleist, M-T Lenz, R-D Peek


Co-operative research project on L-joint testing. Sampling after 4 years exposure
1987 - IRG/WP 2274
In September 1983, Document No: IRG/WP/2208 was distributed giving guidance on sampling after 8 months exposure and including tables on which to record the test results. Similarly in September 1984, Document No: IRG/WP/2233 was distributed concerning sampling after 18 months exposure. No major problems have been notified to Princes Risborough Laboratory concerning the sampling method. It is therefore proposed that the last sampling, after 4 years exposure for all those L-joints exposed in 1983, should proceed using the same methodology, during April or May 1987 as agreed at IRG-17 in Avignon.
J K Carey, A F Bravery


Sub-group on Basidiomycete tests: Experimental programme and schedule of participants
1980 - IRG/WP 2145
The objectives of this IRG COLLABORATIVE BASIDIOMYCETE TEST are to compare results and assess reproducibility of a miniaturised wood block toxicity test when undertaken by different workers in different laboratories. The co-operative scientists and institutes are: Dr K Messner from Vienna (Austria); Dr R S Smith from Forintek (Canada); Mr J Hansen from Sadolins (Denmark); Mr B Jensen from Gori (Denmark); Professor M Fougerousse from CTFT (France); Professor M Gersonde from BAM (Germany); Professor O Wälchli from EMPA (Switzerland); Dr A F Bravery from PRL (UK); Dr A Lewis from Hicksons (UK); Dr C Coggins from Rentokil (UK) Miss J M Taylor from Protim (UK); Dr E L Schmidt from Mississippi (USA). Scheme: Each laboratory to receive samples of PCP and CCA (as Tanalith CT 106) from PRL for comparable tests using mini-block system and own preferred method if desired. Wood blocks: Scots pine sapwood (Pinus sylvestris) 30x10x5 mm³ annual rings (2-6 per cm) parallel with 5 mm face (see Appendix A for suggested cutting scheme). Six replicates per concentration and additional three controls. Two treated and one untreated per culture vessel recommended. Preservative: PCP (in analar toluene) - PRL to despatch; CCA - (sample of Tanalith CT 106) - PRL to despatch. Concentration: PCP: 0, 0.03, 0.06, 0.125, 0.25, 0.5 and 1.0% W/W; CCA: 0, 0.01, 0.04, 0.1, 0.25, 0.6 and 1.5% W/W. Test fungi: European standard strain Coniophora puteana (BAM 15) for PCP and CCA (PRL to despatch). Additional strains as preferred by participants. Incubation: 6 weeks at 22°C ± 1°C and 65 rh ± 5%. Care must be taken to ensure that the humidity in closed incubation cabinets does not exceed the figure given. Medium: 5% malt extract agar (2% agar). Optionally participants may wish to use in addition a sterilised soil/wood feeder block system for comparison. Sterilised filter papers have been used successfully as feeder strips in the petri-dish system. Culture vessels: Petri dishes (90x16 mm approximately) each containing 20 ml agar medium or sterilised soil if preferred. If alternative culture vessels are employed in comparative tests, they should provide a broadly similar surface area and ratio of amount of wood substance to growth medium. Supports: PVC or nylon mesh recommended (approximately 5-7 mm² openings and 2 mm thick). Sterilisation: PCP treated blocks must not be sterilised using propylene or ethylene oxide - radiation is preferred (see Appendix B). Scale: 7 concentrations: 6 replicants per concentration + 3 controls; 7x9 = 63 blocks per preservative / per fungus; 3 culture vessels per concentration = 21 culture vessels per preservative / per fungus. Results: A suggested format for summarising results is attached. Completed results sheets should be returned to Dr A F Bravery as soon as possible in order that a collation can be prepared for the next meeting of IRG in Yugoslavia (May 1981).
A F Bravery


Preservation of basidiomycete hyphae in ancient waterlogged wood materials
1992 - IRG/WP 92-1536
Studies on waterlogged archaeological wood show that basidiomycete hyphae may persist as long as 800 years. In two pine wood samples with Phellinus pini heartrot, one from the foremast of the ship Vasa and the other from a bulwark constructed in the first part of the 12th century, numerous resin covered hyphae were observed. Hyphae with clamp connections that were associated with brown and white rot decay were observed in other samples from the bulwark.
T Nilsson, G F Daniel


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