IRG Documents Database and Compendium


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EPR observation of Mn(II) in decayed wood
2002 - IRG/WP 02-10443
Preliminary Electron Paramagnetic Resonance (EPR) studies of copper/chromium (CC) treated spruce wood, subjected to laboratory soil decay test showed increased intensities of Mn(II) EPR signal in partially decayed specimens. Several reasons might be responsible for this phenomenon: Mn(II) could originate from the wood after being released from the typical ligand binding, from reduction of other Mn...
M Petric, M Humar, P Kalan, F Pohleven, U Schoknecht, M Šentjurc


Detection of increased metal cations after wood decay using Chromeazurol-S
1997 - IRG/WP 97-20112
Chromeazurol-S (CAS) is a dye used for the spectrophotometric determination of metals, mainly aluminum (Al), berillium (Be) and copper (Cu). CAS has been widely used for determining the penetration of copper containing preservatives like CCA (AWPA A3-96). Additional uses include: i) detection of utility pole decay (Esyln, 1979), ii) a chemical spot test for aluminum in wood (Kukachka and Miller, 1...
F Green III, U Srinivasan, R B Miller


Field study: Wood degradation pattern in buildings and utility poles in tropical climates of Nigeria
1992 - IRG/WP 92-1521
The paper is the result of five years field study of wood degradation patterns in three ecological forest zones (Guinea Savannah, Tropical rain forest and mangrove forest zones, respectively). It involved 800 residential buildings and 700 electric overhead transmission poles. The methodology used was a modified Eslyn (6) test. A high rate of Ascomycetes and Basidiomycetes attack especially on the ...
E O Onuorah


Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt


Effects of the specimen position on fungal colonisation and wood decay by en 113 test fungi
1998 - IRG/WP 98-20136
For testing wood preservatives according to EN 113 it is common practice to plant the test blocks on neutral supports in order to prevent (1) a diffusion of chemicals into the agar medium and (2) an excessive moistening of the specimens. The procedure was employed in EN 350-1 for testing the natural durability of solid wood. It turned out to be of problematic nature because of the individual requ...
G Kleist, M-T Lenz, R-D Peek


Evaluation of white-rot fungal growth on Southern Yellow pine wood chips pretreated with blue-stain fungi
2000 - IRG/WP 00-10349
White-rotting basidiomycetes do not colonize on southern yellow pine. This study seeks to reduce the resinous extractive content of southern yellow pine by treating it with blue stain fungi. The mycelial growth of wood-inhabiting ligninolytic white-rot fungi can be achieved on pretreated southern yellow pine wood. Aureobasidium, Ceratocystis, and Ophiostoma spp. removed 70% to 100% of the extracti...
S C Croan


Influence of the decay of spruce chips by the selected fungi on their chemical structure and the pulp properties
2004 - IRG/WP 04-10510
The paper focuses on changes in the weight and in the molecular structure of spruce chips submitted to long-term storing as well as to medium- and long-term model rotting degradation caused by some chosen fungi identified at storing processes on the pile. For the model decay of chips under laboratory conditions during 3 or 6 weeks the white-rot fungi: Phanerochaete chrysosporium, Heterobasidion an...
R Solár, L Reinprecht, A Geffert, F Kacík


Biological resistance of wood-based composites under protected, aboveground conditions
2005 - IRG/WP 05-20312
Five kinds of wood-based composites (density fiberboard=MDF, hardwood plywood=HP, softwood plywood =SP, particleboard=PB and aspen oriented strand board=OSB) were tested for their resistance against subterranean termites and decay under protected aboveground conditions. The exposure method was previously developed for evaluating performance of sill plates (dodai) in Japanese homes. Ten replicates ...
K Tsunoda


Uptake of copper by mycelium of wood decay fungi growing on copper S-substituted thioglycolate containing nutrient media
1998 - IRG/WP 98-10291
Mycelia of Trametes versicolor, Coniophora puteana and Poria monticola were grown on potato dextrose agar (PDA) media, containing various concentrations of copper N,N-dimethyldithiocarbamoylacetate (which may be regarded as a copper S-substituted thioglycolate). The tested copper compound revealed relatively low fungicidal activity. After 13-18 day growing period, we determined concentrations of a...
M Humar, M Petric, F Pohleven, P Kalan


Use of immunoblotting for the analysis of wood decay basidiomycetes
1988 - IRG/WP 2307
Immunoblotting methods, in particular dot and Western blotting have been used to investigate features of a variety of wood decay organisms, in particular Coriolus versicolor, Lentinus lepideus and Serpula lacrymans. Antisera to each of these decay organisms has been produced by immunisation of rabbits with liquid culture grown hyphae. These antisera, after appropriate preabsorption with sawdust ha...
J W Palfreyman, H Glancy, D Button, A Vigrow, A J Score, B King


A comparison of fungal strains used in the bioassay of wood preservatives
1984 - IRG/WP 2220
Previously published data are presented relating to a number of strains of wood-destroying basidiomycetes (Coniophora puteana, Coriolus versicolor, Gloeophyllum abietinum, Gloeophyllum sepiarium, Gloeophyllum trabeum, Lentinus lepideus, Poria placenta, Fibroporia vaillantii and Serpula lacrymans) commonly used as test fungi in the bioassay of wood preservatives. The data, which has not been statis...
J Wazny, H Greaves


Exposure of preservative treated wood to terrestrial microcosms, pure cultures of fungi and in the field
1997 - IRG/WP 97-20114
Small stakes (5x10x100 mm3) of treated and untreated Pinus sylvestris sapwood were exposed in terrestrial microcosms with different soils, two taken from test fields in Sweden, one soil from a conifer forest and one garden compost soil. Stakes of the same size were also exposed to pure cultures of the brown and white rot fungi, Postia placenta and Phanerochaete chrysosporium. After exposure, weigh...
M-L Edlund


Screening of fungal strains for wood extractive degradation
1998 - IRG/WP 98-10254
Fungal strains were screened for their ability to degrade apolar extractives in wood from scots pine. The degradation of total wood extractives by 91 different strains was monitored in stationary batch assays incubated for 6 weeks. The results obtained show that the ability of wood-inhabiting fungi to utilize wood extractives varied greatly, even for different isolates of the same species. Fungal ...
J Dorado, M J Martinez-Inigo, T A van Beek, F W Claassen, J B P A Wijnberg, R Sierra-Alvarez


The influence of timber species and preservative treatment on spore germination of some wood-destroying Basidiomycetes
1988 - IRG/WP 2300
Basidiospores from six wood decay fungi exhibited varying germination rates on untreated softwood and hardwood blocks. Germination inhibition of all test fungi was recorded on pine sapwood. No preference for a certain timber species by a particular fungus was evident. Whereas almost complete inhibition of germination occurred on wood treated with a quarternary-ammonium based wood preservative, mos...
B M Hegarty, G Buchwald


Influence of the content of organic matter of soil on the degradation of wood by soft rot fungi
1970 - IRG/WP 27
The degradation of wood specimen burried in the soil by soft rot fungi is influenced by a number of factors. These are in particular the water holding capacity, water content, content of organic matter, pH, content of mineral nutritive substances etc. The results of the interlaboratory tests, carried out within the IRG/WP let presume that the intensity of wood degradation occurs proportionally inv...
O Wälchli


The use of fumigants for controlling decay of wood: a review of their efficacy and safety
1989 - IRG/WP 3525
Volatile agricultural chemicals (fumigants) such as chloropicrin (trichloronitromethane) and sodium n-methyldithiocarbamate have been used in the United States for controlling internal decay of large dimension wood products for over 20 years. This usage has been concentrated in the electric utility industry, but fumigants are increasingly applied to protect bridge timbers, marine piling, and even ...
J J Morrell


A Novel Type of Multiple Cavity Attack in Wood Cell Walls of Heat-treated Timber Exposed in Seawater – Preliminary Observations
2004 - IRG/WP 04-10523
Samples of untreated and heat-treated Norway spruce and Douglas fir were submerged in Langstone Harbour, Portsmouth for 4 years at a depth of ca. 0.3 metres. The heat-treated samples had been prepared using the Plato process. Samples were initially assessed for the severity of marine borer damage and were then examined microscopically for evidence of microbial decay. Longitudinal and transverse wo...
R A Eaton, C Björdal, T Nilsson


Antagonism of Gliocladium virens against wood decay fungi
1995 - IRG/WP 95-10102
Antagonistic abilities of a commercial biofungicide, Gliocladium virens (GL-21, Grace) were evaluated against three white-rot fungi, Trametes versicolor, Phlebia brevispora, Irpex lacteus, and three brown-rot fungi, Postia placenta, Neolentinus lepideus, Gloeophyllum trabeum. In dual culture of Gliocladium virens and wood decay fungi, Gliocladium virens rapidly overgrow the decay fungi and killed ...
T L Highley, L Ferge


A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl


Chitin synthetase activity in cellular fractions of wood-decay fungi
1992 - IRG/WP 92-1524
This study was an attempt to explain the variability of in vivo sensitivity of wood decay fungi to the chitin synthetase (CS) inhibitor, polyoxin, by comparing CS distribution in cellular fractions. The five species studied were the brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, Fibuloporia vaillantii, and the white-rot fungi Schizophyllum commune and Ganoderma applanatum. The CS activi...
B R Johnson, S C Croan, B Illman


Antagonism of Scytalidium lignicola against wood decay fungi
1989 - IRG/WP 1392
Antagonistic abilities of Scytalidium lignicola against white- and brown-rot wood decay fungi were evaluated. Scytalidium lignicola did not produce inhibition zones but overgrew the decay fungi on a malt-agar medium and in most cases killed them. Pretreatment of Douglas-fir and Southern pine blocks with Scytalidium lignicola prevented decay. Blocks that were heated or treated with propylene oxide ...
T L Highley


Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium
1988 - IRG/WP 1364
The degradation of lignin in native lignocellulosic substrates by white rot fungi is poorly understood. Biochemical studies have shown the involvement of a number extracellular ligninolytic enzymes released by white rot fungi which are capable of the oxidative conversion of DHP's (lignin model compounds) in vitro, but to date conclusive evidence for occurrence of these enzymes in wood und...
G F Daniel, T Nilsson, B Pettersson


Evaluation of decay fungi in Norwegian buildings
2005 - IRG/WP 05-10562
During a period of 2 years and 3 months (1 January 2001 - 20 March 2003) Mycoteam had 3161 consultations in buildings in southern Norway, 1428 revealing damage from decay fungi. One consultation often revealed several occurrences of fungi, and the total number of occurrences of decay fungi was 3434. Thirty-five different species/genera/groups of decay fungi were recorded. During this period brown ...
G Alfredsen, H Solheim, K Mohn Jenssen


Effects of heart wood extractives in Dahoma (Piptadeniastrum africanum) on decay resistance to white- and brown-rot fungi
2004 - IRG/WP 04-10536
Dahoma (Piptadeniastrum africanum) is a durable but a lesser utilised tropical hardwood species from Ghana that has the potential of being used as an alternative to Iroko (Milicia excelsa) for many end uses. However, the timber gives off a strong odour especially when wet and during processing. The high durability and the strong odour of the timber could be due to the presence of extractives in th...
Zeen Huang, K Maher, S A Amartey


Identification and inhibitory effect of volatiles from different ages of a Trichoderma aureoviride culture on selected wood decay fungi.
1995 - IRG/WP 95-10110
The ability of a Trichoderma sp. to produce volatile organic compounds (VOCs) over a four week period of growth was examined and the inhibitory effect of these volatiles against four selected basidiomycetes over the same period was assessed. After trapping, on tubes filled with chromatography packing material, VOCs were analysed on an integrated automated thermal desorbtion mass spectrometer syste...
A Bruce, A Kundzewicz, R E Wheatley


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